{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T12:41:36Z","timestamp":1720874496708},"reference-count":31,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,12,1]]},"abstract":"<jats:p>A human anaplastic thyroid cancer cell line FB-1, derived from a 68-yr-old woman who underwent surgery for anaplastic thyroid cancer, has been established. The spindlelike cells have been proliferating stably for more than 2 yr. Karyotype analysis shows many abnormalities and many marker chromosomes have been observed. Heterotransplant of FB-1 cells into severe combined immunodeficient mice has resulted in rapidly growing tumors classified as anaplastic carcinomas, although 50% have shown areas with a trabecular pattern. FB-1 cells failed to express messenger RNA for thyroglobulin; TSH-receptor; thyroperoxidase, and placental angiogenic growth factor. Conversely, PAX8 and thyroid transcription factor 1, whose expression is thyroid specific, was kept in an FB-1 cell line at a level comparable with that observed in normal thyroid tissue. In addition, the present cell line expressed high levels of messenger RNA for high-mobility group proteins (Y) and -C. The in vitro study revealed that FB-1 cells are able to produce high levels of interleukin (IL)-8 and medium amount of IL-6, whereas no release of IL-1-\u03b1, IL-1-\u03b2, and IL-4 was observed. No modulation of cell proliferation and DNA synthesis in FB-1 cells has been observed after the addition of exogenous IL-6.<\/jats:p>","DOI":"10.1210\/jcem.82.12.4444","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:01:01Z","timestamp":1389200461000},"page":"4094-4100","source":"Crossref","is-referenced-by-count":3,"title":["Cytokine Production by a New Undifferentiated Human Thyroid Carcinoma Cell Line, FB-11"],"prefix":"10.1210","volume":"82","author":[{"given":"Lisa","family":"Fiore","sequence":"first","affiliation":[{"name":"Dipartimento di Oncologia (L.F., L.E.P., G.F., R.G., F.B.), Pisa, 56100, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luca E.","family":"Pollina","sequence":"additional","affiliation":[{"name":"Dipartimento di Oncologia (L.F., L.E.P., G.F., R.G., F.B.), Pisa, 56100, 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