{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T02:07:06Z","timestamp":1784945226844,"version":"3.55.0"},"reference-count":36,"publisher":"The Endocrine Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,8,1]]},"abstract":"<jats:p>In the present study, we examined the embryonic regulation of\u03b2 3 integrin in human endometrial epithelial cells (EEC) at the protein level and analyzed putative embryonic factors responsible for this regulation. The model employed is based on a clinical in vitro fertilization program in which single human embryos were cocultured with EEC until blastocyst stage and then transferred back to the uterus. After embryo transfer, EEC wells were divided according to the embryonic status reached: EEC with embryos that achieved the blastocyst stage, EEC with arrested embryos, and EEC without embryos. Immunostaining for \u03b23 was positive in plasma membrane of EEC. Flow cytometry showed a mean percentage of\u03b2 3-stained cells of 24.1 \u00b1 5.7 in EEC cocultured with embryos that achieved the blastocyst stage (n = 13) vs. 9.5 \u00b1 1.6 (P &amp;lt; 0.05) in those EEC cultured with arrested embryos (n = 12). Immunostaining for \u03b11 and \u03b14 integrins was negative in EEC monolayers studied, regardless of the presence or absence of embryos, and these findings were confirmed by flow cytometry. The possibility that the embryonic IL-1 system and leukemia inhibitory factor were involved in the endometrial \u03b23 up-regulation was investigated by neutralizing experiments demonstrating a significant inhibition of \u03b23-stained cells when EEC monolayers were cultured in the presence of EEC\/blastocyst-conditioned media with (n = 4) vs. without (n = 8) antihuman interleukin (IL)-1\u03b1 + IL-1\u03b2 (1.65% vs. 14.6%; P &amp;lt; 0.05). Dose-response experiments further demonstrated an up-regulation of \u03b23 positive cells when IL-1\u03b1 + IL-1\u03b2 were added to the medium at a concentration of 10 pg\/mL compared with control medium without added cytokines (40% vs. 20%, n = 4). The functional relevance of the EEC \u03b23 up-regulation was tested using a mouse blastocyst adhesion assay. More mouse blastocysts attached to EEC previously in contact with human blastocyst (72.7%) compared with those EEC previously in contact with arrested embryos (40%). Our results demonstrate the selective effect of a developing human embryo on EEC expression of \u03b23, which is maximal when a human blastocyst instead of an arrested embryo is considered. Furthermore, the embryonic IL-1 system seems to be involved in the EEC\u03b2 3 up-regulation, reinforcing the concept of precise paracrine cross-talk between blastocyst and endometrial epithelium during embryonic implantation.<\/jats:p>","DOI":"10.1210\/jcem.82.8.4153","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:02:30Z","timestamp":1389200550000},"page":"2607-2616","source":"Crossref","is-referenced-by-count":11,"title":["Embryonic Regulation of Integrins \u03b23,\u03b1 4, and \u03b11 in Human Endometrial Epithelial Cells in Vitro1"],"prefix":"10.1210","volume":"82","author":[{"given":"Carlos","family":"Simo\u0301n","sequence":"first","affiliation":[{"name":"Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, (C.S., A.M., M.J.G., J.R., A.P.), Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MarI\u0301a Jose\u0301","family":"Gimeno","sequence":"additional","affiliation":[{"name":"Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, (C.S., A.M., M.J.G., J.R., A.P.), Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amparo","family":"Mercader","sequence":"additional","affiliation":[{"name":"Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, (C.S., A.M., M.J.G., J.R., A.P.), Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 Enrique","family":"O\u2019Connor","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology (J.E.O.), Valencia University, Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"RemohI\u0301","sequence":"additional","affiliation":[{"name":"Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, (C.S., A.M., M.J.G., J.R., A.P.), Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Lake","family":"Polan","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology (M.L.P.), Stanford University Medical Center, Stanford, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Pellicer","sequence":"additional","affiliation":[{"name":"Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, (C.S., A.M., M.J.G., J.R., A.P.), Valencia, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071620081030700_R1"},{"key":"2020071620081030700_R2","first-page":"405","article-title":"Cytokines-adhesion molecules-invasive proteinases. 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