{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T11:29:28Z","timestamp":1676978968294},"reference-count":54,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,1,1]]},"abstract":"<jats:p>NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (PGDH) is the key catabolic enzyme controlling levels of biologically active PGs. PGDH is localized to syncytiotrophoblast in placenta, and to trophoblast cells in chorion. To examine the regulation of PGDH by steroids and to determine any changes with labor, we obtained placenta and chorion from term elective cesarean section or spontaneous delivery and isolated trophoblast cells using a Percoll density gradient. Cells were treated with varying concentrations of cortisol, progesterone, the synthetic progestins R5020, and medroxyprogesterone acetate with or without RU486 or the specific progesterone receptor antagonist, onapristone, and the 3\u03b2-hydroxysteroid dehydrogenase inhibitor, trilostane. The activity of PGDH was assessed by measurement of 13,14-dihydro-15-keto-PGF2\u03b1. PGDH messenger ribonucleic acid was quantified by in situ hybridization and computerized image analysis.<\/jats:p><jats:p>The basal output of 13,14-dihydro-15-keto-PGF2\u03b1 was lower in placenta or chorion collected at spontaneous labor than in that obtained at elective cesarean section. Cortisol had a significant dose-dependent inhibitory effect on PGDH activity in both placental and chorion trophoblast cells and significantly decreased levels of PGDH messenger ribonucleic acid. Responses were similar between tissues from laboring and nonlaboring women. PGDH activity was increased by R5020 and medroxyprogesterone acetate and was inhibited by RU486, onapristone, and trilostane. We conclude that cortisol inhibits PGDH activity and expression and that progestagens increase PGDH activity in human chorion and placenta.<\/jats:p>","DOI":"10.1210\/jcem.84.1.5399","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:03:29Z","timestamp":1389200609000},"page":"291-299","source":"Crossref","is-referenced-by-count":3,"title":["Steroid Regulation of Prostaglandin Dehydrogenase Activity and Expression in Human Term Placenta and Chorio-Decidua in Relation to Labor1"],"prefix":"10.1210","volume":"84","author":[{"given":"Falguni A.","family":"Patel","sequence":"first","affiliation":[{"name":"Medical Research Council Group in Fetal and Neonatal Health and Development, Departments of Physiology and Obstetrics and Gynecology (F.A.P, V.L.C., J.R.G.C.), University of Toronto, Toronto, Ontario, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicki L.","family":"Clifton","sequence":"additional","affiliation":[{"name":"Medical Research Council Group in Fetal and Neonatal Health and Development, Departments of Physiology and Obstetrics and Gynecology (F.A.P, V.L.C., J.R.G.C.), University of Toronto, Toronto, Ontario, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Chwalisz","sequence":"additional","affiliation":[{"name":"Research Laboratories of Schering AG (K.C.), Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R. G.","family":"Challis","sequence":"additional","affiliation":[{"name":"Medical Research Council Group in Fetal and Neonatal Health and Development, Departments of Physiology and Obstetrics and Gynecology (F.A.P, V.L.C., J.R.G.C.), University of Toronto, Toronto, Ontario, Canada;"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071620492088800_R1","first-page":"45","article-title":"Role of prostaglandins, prostacyclin, and thromboxanes in the physiologic control of the uterus and parturition.","volume":"4","author":"Novy","year":"1980","journal-title":"Semin Perinatol"},{"key":"2020071620492088800_R2","first-page":"107","article-title":"The mechanism(s) of human parturition.","volume":"6","year":"1984","journal-title":"J Dev Physiol"},{"key":"2020071620492088800_R3","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0090-6980(78)90177-6","article-title":"Specific production of prostaglandin E2 by human amnion in vitro.","volume":"15","author":"Mitchell","year":"1978","journal-title":"Prostaglandins"},{"key":"2020071620492088800_R4","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/0002-9378(81)90312-4","article-title":"Initiation of parturition. XII. Biosynthesis and metabolism of prostaglandins in human fetal membranes and uterine decidua.","volume":"139","author":"Okazaki","year":"1981","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R5","first-page":"E832","article-title":"Prostaglandin-endoperoxide H synthase-2 expression and activity increases with term labor in human chorion","volume":"272","author":"Mijovic","year":"1997","journal-title":"Am J Physiol"},{"key":"2020071620492088800_R6","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1210\/jcem-57-4-694","article-title":"Prostaglandin output in relation to parturition by cells dispersed from human intrauterine tissues.","volume":"57","author":"Olson","year":"1983","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R7","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/0002-9378(85)90281-9","article-title":"Changes in the synthesis and metabolism of prostaglandins by human fetal membranes and decidua at labor.","volume":"151","author":"Skinner","year":"1985","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R8","first-page":"1396","article-title":"Prostaglandin endoperoxide-H synthase (PGHS) activity and immunoreactive PGHS-1 and PGHS-2 levels in human amnion throughout gestation, at term and during labor.","volume":"78","author":"Teixeira","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R9","doi-asserted-by":"crossref","first-page":"520","DOI":"10.3109\/00016349309058155","article-title":"The amnion produces little of the prostaglandin E2 detected on the decidual side of human fetal membranes.","volume":"72","author":"Sullivan","year":"1993","journal-title":"Acta Obstet Gynecol Scand"},{"key":"2020071620492088800_R10","first-page":"759","article-title":"The dynamics of prostaglandin metabolism in human fetal membranes and decidua around the time of parturition.","volume":"77","author":"Mitchell","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R11","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1016\/0002-9378(90)90642-K","article-title":"The role of fetal membranes in regulating production, transport, and metabolism of prostaglandin E2 during labor.","volume":"163","author":"McCoshen","year":"1990","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R12","first-page":"101","volume":"15","year":"1979"},{"key":"2020071620492088800_R13","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1016\/0002-9378(90)90603-5","article-title":"Immunocytochemical distribution and localization of 15-hydroxyprostaglandin dehydrogenase in human fetal membranes, decidua, and placenta.","volume":"163","author":"Cheung","year":"1990","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R14","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1159\/000294868","article-title":"Localization of 15-hydroxyprostaglandin dehydrogenase in human fetal membranes, decidua, and placenta during pregnancy.","volume":"33","author":"Cheung","year":"1992","journal-title":"Gynecol Obstet Invest"},{"key":"2020071620492088800_R15","doi-asserted-by":"crossref","first-page":"1337","DOI":"10.1016\/0002-9378(86)90170-5","article-title":"Changes in prostaglandin transfer across human fetal membranes obtained after spontaneous labor.","volume":"155","author":"Nakla","year":"1986","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R16","doi-asserted-by":"crossref","first-page":"425","DOI":"10.3109\/00016349109007154","article-title":"Metabolism of prostaglandin E2 on the fetal and maternal sides of intact fetal membranes.","volume":"70","author":"Sullivan","year":"1991","journal-title":"Acta Obstet Gynecol Scand"},{"key":"2020071620492088800_R17","first-page":"969","article-title":"15-Hydroxyprostaglandin dehydrogenase (PGDH): implications in preterm labor with and without ascending infection.","volume":"82","author":"Meir","year":"1997","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R18","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0143-4004(97)90081-3","article-title":"Chorionic prostaglandin catabolism is decreased in the lower uterine segment with term labor.","volume":"18","author":"Meir","year":"1997","journal-title":"Placenta"},{"key":"2020071620492088800_R19","first-page":"982","article-title":"Immunohistochemical localization, mRNA abundance and activity of 15-hydroxyprostaglandin dehydrogenase in placenta and fetal membranes during term and pre-term labor.","volume":"78","author":"Sangha","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R20","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0262-1746(87)90074-6","article-title":"Effect of dexamethasone on fetal lung 15-hydroxyprostaglandin dehydrogenase: possible mechanism for the prevention of patent ductus arteriosus by maternal dexamethasone therapy.","volume":"27","author":"Tsai","year":"1987","journal-title":"Prostaglandins Leukotriene Med"},{"key":"2020071620492088800_R21","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1210\/endo-114-3-1033","article-title":"Plasma concentrations, renal excretion, and tissue release of prostaglandins in the rat with dexamethasone-induced hypertension.","volume":"114","author":"Nasjletti","year":"1984","journal-title":"Endocrinology"},{"key":"2020071620492088800_R22","doi-asserted-by":"crossref","first-page":"1853","DOI":"10.1210\/endo-121-5-1853","article-title":"Biphasic effect of dexamethasone on urinary prostaglandins in rats: relation to alterations in renal medulla triglycerides and prostaglandin metabolism.","volume":"121","author":"Erman","year":"1987","journal-title":"Endocrinology"},{"key":"2020071620492088800_R23","first-page":"269","article-title":"Dexamethasone stimulates arachidonic acid conversion to prostaglandin E2 in human amnion cells.","volume":"12","author":"Zakar","year":"1989","journal-title":"J Dev Physiol"},{"key":"2020071620492088800_R24","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/0303-7207(95)03739-X","article-title":"Glucocorticoids stimulate prostaglandin H synthase type-2 (PGHS-2) in the fibroblast cells in human amnion cultures.","volume":"117","author":"Economopoulos","year":"1996","journal-title":"Mol Cell Endocrinol"},{"key":"2020071620492088800_R25","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1210\/endo-98-4-859","article-title":"Progesterone and estrogen control of uterine prostaglandin dehydrogenase activity during deciduomal growth.","volume":"98","author":"Alam","year":"1976","journal-title":"Endocrinology"},{"key":"2020071620492088800_R26","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1111\/j.1476-5381.1975.tb07393.x","article-title":"Enhanced inactivation of prostaglandin E2 by the rabbit lung during pregnancy or progesterone treatment.","volume":"53","author":"Bedwani","year":"1975","journal-title":"Br J Pharmacol"},{"key":"2020071620492088800_R27","author":"Myatt","year":"1983"},{"key":"2020071620492088800_R28","first-page":"407","article-title":"Differentiation of human cytotrophoblast into syncytiotrophoblast in culture.","volume":"2","author":"Kliman","year":"1987","journal-title":"Trophoblast Res"},{"key":"2020071620492088800_R29","doi-asserted-by":"crossref","first-page":"1567","DOI":"10.1210\/endo-118-4-1567","article-title":"Purification, characterization and in vitro differentiation of cytotrophoblasts from human term placentae.","volume":"118","author":"Kliman","year":"1986","journal-title":"Endocrinology"},{"key":"2020071620492088800_R30","doi-asserted-by":"crossref","first-page":"4912","DOI":"10.1210\/endo.138.11.5544","article-title":"Differential regulation of 11\u03b2-hydroxysteroid dehydrogenase type 1 and 2 by nitric oxide in cultured human placental trophoblast and chorionic cell preparation.","volume":"138","author":"Sun","year":"1997","journal-title":"Endocrinology"},{"key":"2020071620492088800_R31","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1016\/0002-9378(89)90930-7","article-title":"Prostaglandin E2 metabolism in the human fetal membranes.","volume":"161","author":"Cheung","year":"1989","journal-title":"Am J Obstet Gynecol"},{"key":"2020071620492088800_R32","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/S0090-6980(74)80107-3","article-title":"Measurement of prostaglandin F2\u03b1 metabolites by radioimmunoassay.","volume":"25","author":"Cornette","year":"1974","journal-title":"Prostaglandins"},{"key":"2020071620492088800_R33","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1530\/jrf.0.0710045","article-title":"Early changes in prostaglandin concentrations in ovine maternal and fetal plasma amniotic fluid and from dispersed cells of intrauterine tissues before the onset of ACTH-induced pre-term labour.","volume":"71","author":"Olson","year":"1984","journal-title":"J Reprod Fertil"},{"key":"2020071620492088800_R34"},{"key":"2020071620492088800_R35","doi-asserted-by":"crossref","first-page":"14888","DOI":"10.1016\/S0021-9258(18)77199-8","article-title":"Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase.","volume":"265","author":"Ensor","year":"1990","journal-title":"J Biol Chem"},{"key":"2020071620492088800_R36","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1016\/0006-291X(91)90677-Y","article-title":"Regulation of synthesis and activity of NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) by dexamethasone and phorbol ester in human erythroleukemia (HEL) cells.","volume":"177","author":"Xun","year":"1991","journal-title":"Biochem Biophy Res Commun"},{"key":"2020071620492088800_R37","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/288269a0","article-title":"Anti-inflammatory steroids reduce PG synthetase activity and enhance PG breakdown.","volume":"288","author":"Moore","year":"1980","journal-title":"Nature"},{"key":"2020071620492088800_R38","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1530\/eje.0.1330527","article-title":"Changes in prostaglandin synthesis and metabolism associated with labor, and the influence of dexamethasone, RU486 and progesterone.","volume":"133","author":"Brennand","year":"1995","journal-title":"Eur J Endocrinol"},{"key":"2020071620492088800_R39","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1139\/y88-125","article-title":"Cyclooxygenase products formed by primary cultures of cells from human chorion laeve: influence of steroids.","volume":"66","author":"Gibb","year":"1988","journal-title":"Can J Physiol Pharmacol"},{"key":"2020071620492088800_R40","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1073\/pnas.69.3.533","article-title":"Human placental 15-hydroxyprostaglandin dehydrogenase.","volume":"69","year":"1972","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071620492088800_R41","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S0090-6980(74)80011-0","article-title":"Partial purification of human placental 15-hydroxy-prostaglandin dehydrogenase: kinetic properties.","volume":"5","author":"Schlegel","year":"1974","journal-title":"Prostaglandins"},{"key":"2020071620492088800_R42","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0960-0760(90)90377-W","article-title":"Antiprogestagenic inhibition of uterine prostaglandin inactivation: a permissive mechanism for uterine stimulation.","volume":"37","author":"Kelly","year":"1990","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071620492088800_R43","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1093\/oxfordjournals.humrep.a138124","article-title":"The effects of mifepristone (RU486) on prostaglandin dehydrogenase in decidual and chorionic tissue in early pregnancy.","volume":"8","author":"Cheng","year":"1993","journal-title":"Hum Reprod"},{"key":"2020071620492088800_R44","author":"Mitchell","year":"1988"},{"key":"2020071620492088800_R45","first-page":"375","article-title":"Administration of RU486 for 8 days in normal volunteers: antiglucocorticoid effect with no evidence of peripheral cortisol deprivation.","volume":"78","author":"Bertagna","year":"1994","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R46","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0960-0760(91)90176-6","article-title":"Regulation of basal ACTH secretion by corticosterone is mediated by both type I (MR) and type II (GR) receptors in rat brain.","volume":"40","author":"Bradbury","year":"1991","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071620492088800_R47","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/0022-4731(85)90401-7","article-title":"RU 38386: a potent antiglucocorticoid in vitro and in vivo.","volume":"23","author":"Gagne","year":"1985","journal-title":"J Steroid Biochem"},{"key":"2020071620492088800_R48","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1210\/jcem-66-2-290","article-title":"The antiglucocorticoid and antiprogestin steroid RU486 suppresses the adrenocorticotropin response to ovine corticotrophin-releasing factor in man.","volume":"66","author":"Laue","year":"1988","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620492088800_R49","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0022-4731(84)90216-4","article-title":"RU 38486: potent antiglucocorticoid activity correlated with strong binding to the cytosolic glucocorticoid receptor followed by an impaired activation.","volume":"20","author":"Moguilewsky","year":"1984","journal-title":"J Steroid Biochem"},{"key":"2020071620492088800_R50","first-page":"200","article-title":"Antagonist and agonist effects of the antiprogesterone RU486.","volume":"50","year":"1989","journal-title":"Ann Endocrinol (Paris)"},{"key":"2020071620492088800_R51","first-page":"R710","article-title":"Predominately glucocorticoid agonist actions of RU486 in young specific-pathogen-free Zucker rats","volume":"271","author":"Havel","year":"1996","journal-title":"Am J Physiol"},{"key":"2020071620492088800_R52","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0262-1746(87)90094-1","article-title":"Effects of steroids on renal NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity in ovariectomized rats.","volume":"29","year":"1987","journal-title":"Prostaglandins Leukotriene Med"},{"key":"2020071620492088800_R53","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1016\/0006-2952(85)90397-1","article-title":"Induction of a decrease in renal NAD+-dependent 15-hydroxyprostaglandin dehydrogenase activity by estradiol in rats.","volume":"34","author":"Chang","year":"1985","journal-title":"Biochem Pharmacol"},{"key":"2020071620492088800_R54","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0952-3278(94)90159-7","article-title":"Prostaglandin inactivation is increased in endometrium after exposure to clomiphene.","volume":"50","author":"Kelly","year":"1994","journal-title":"Prostaglandins Leukotriene Essent Fatty Acids"}],"container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/84\/1\/291\/10402237\/jcem0291.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/84\/1\/291\/10402237\/jcem0291.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T23:53:12Z","timestamp":1647906792000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/84\/1\/291\/2866242"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,1,1]]},"references-count":54,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1999,1,1]]}},"URL":"https:\/\/doi.org\/10.1210\/jcem.84.1.5399","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,1,1]]}}}