{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:35:39Z","timestamp":1761708939872,"version":"3.40.4"},"reference-count":20,"publisher":"The Endocrine Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:p>PGE2 is known to induce uterine contraction by increasing intracellular Ca2+. In the present study, to investigate other functions of PGE2 in human uterus, two EP3 isoforms were isolated by the RT-PCR method using human uterus polyadenylated ribonucleic acid (RNA). These EP3 isoforms, named EP3-V and EP3-VI, are composed of 402 and 393 amino acid residues, respectively, which are unique compared with EP3 isoforms of other species. Their N-terminal 359 amino acid residues are identical to those of previously reported human EP3 isoforms, whereas the two isoforms contained a novel amino acid sequence in their C-terminal tails. The dissociation constant values of EP3-V and EP3-VI for PGE2 were 3.9 and 1.4 nmol\/L, respectively, which were consistent with those of previously reported EP3 isoforms. Signaling experiments revealed that M&amp;B28767, an EP3 agonist, not only inhibited forskolin-induced cAMP concentrations, but also activated mitogen-activated protein kinase in Chinese hamster ovary cells stably expressing EP3-V and EP3-VI. These responses were abolished by treatment with pertussis toxin. In addition, M&amp;B28767 increased cAMP concentrations in EP3-VI-expressing cells, whereas it did not in EP3-V-expressing cells. M&amp;B28767 did not stimulate phosphoinositide turnover in EP3-V- or EP3-VI-expressing cells. EP3-V and EP3-VI messenger RNAs (mRNAs) were detected abundantly in human uterus, whereas weak, but substantial, bands were detected in the lung and kidney in RT-PCR specific for each mRNA. In situ hybridization revealed EP3-V and EP3-VI mRNAs in the human myometrium, but not in the endometrium. The present study suggests that EP3-V and EP3-VI are possibly involved in the proliferation of cells in human myometrium.<\/jats:p>","DOI":"10.1210\/jcem.85.11.6989","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:05:14Z","timestamp":1389200714000},"page":"4315-4322","source":"Crossref","is-referenced-by-count":23,"title":["Multiple Signal Transduction Pathways through Two Prostaglandin E Receptor EP3 Subtype Isoforms Expressed in Human Uterus"],"prefix":"10.1210","volume":"85","author":[{"given":"Masato","family":"Kotani","sequence":"first","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issei","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayoshi","family":"Suganami","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsunekazu","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Gynecology and Obstetrics (T.M., N.S.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Muro","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Sugawara","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunao","family":"Yoshimasa","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norimasa","family":"Sagawa","sequence":"additional","affiliation":[{"name":"Gynecology and Obstetrics (T.M., N.S.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuh","family":"Narumiya","sequence":"additional","affiliation":[{"name":"Pharmacology (S.N.), Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuwa","family":"Nakao","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Clinical Science (M.K., I.T., Y.O., T.S., S.M., Y.Ya., A.S., Y.Yo., K.N.), Kyoto 606-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"year":"1990","key":"2020071617561304100_R1","article-title":"Lipids-derived autacoids. 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