{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,2]],"date-time":"2022-04-02T01:25:15Z","timestamp":1648862715999},"reference-count":39,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>Human term myometrium is poorly characterized as a source of proinflammatory mediators involved in parturition. We have investigated the basal expression of cytokines in myometrium, as well as the effects of CRH and lipopolysaccharide (LPS) on cytokine release.<\/jats:p><jats:p>Explants from term myometrium were challenged with CRH or LPS (1\u03bc g\/mL each) in short-term tissue culture. Interleukin (IL)-1\u03b2, IL-6, IL-8, and tumor necrosis factor (TNF)\u03b1 concentrations in the medium were quantified by enzyme immunoassay. The major cytokines released after 24 h were IL-6 and IL-8. All cytokines investigated were stimulated significantly by LPS (P &amp;lt; 0.05) but not by CRH. Messenger RNA levels of these cytokines were investigated by RT-PCR. IL-1\u03b2 and IL-6 messenger RNA were present in preterm and term myometrium before and during labor, whereas IL-8 and TNF\u03b1 were expressed only by myometrium in active labor. Furthermore, myometrial CRH receptors and macrophages were characterized immunohistochemically.<\/jats:p><jats:p>We conclude that human term myometrium is a site of production of proinflammatory cytokines and is involved in the inflammation-like reactions mediating the birth process. Cytokine release in term myometrium seems not to be under control of CRH.<\/jats:p>","DOI":"10.1210\/jcem.85.12.7006","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:05:05Z","timestamp":1389200705000},"page":"4859-4865","source":"Crossref","is-referenced-by-count":3,"title":["Formation of Proinflammatory Cytokines in Human Term Myometrium Is Stimulated by Lipopolysaccharide But Not by Corticotropin-Releasing Hormone1"],"prefix":"10.1210","volume":"85","author":[{"given":"Bernd","family":"Sehringer","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology II, University of Freiburg, Germany D-79106"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang R.","family":"Scha\u0308fer","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology II, University of Freiburg, Germany 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