{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T03:09:16Z","timestamp":1648782556630},"reference-count":49,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:p>Ovarian cancer originates mainly from surface epithelial cells, which are potential targets of estrogen action. Using immunohistochemistry and RT-PCR analysis, aromatase (estrogen synthetase) can be detected in human ovarian surface epithelial tumors. In this study, we functionally characterized the aromatase expressed in a primary cell culture, normal human ovarian surface epithelial (HOSE) 17. The apparent Km and Vmax values were determined to be 5.8 \u00b1 0.5 nm, and 0.3 \u00b1 0.0 pmol\/mg\u00b7h, respectively. The aromatase activity in HOSE 17 cells can be induced effectively by phorbol esters and forskolin, suggesting that estrogen biosynthesis in HOSE 17 cells is mainly regulated through protein kinase C- and protein kinase A-mediated mechanisms. Exon I-specific RT-PCR revealed that phorbol esters predominantly up-regulated promoter II. Whereas forskolin treatment increased exon I.3A-containing messenger RNA, the aromatase activity remained low in the cells treated with this agent. In vitro transcription\/translation analysis using plasmids containing T7 promoter and the human snail gene (SnaH) as a reporter capped with different untranslated exon Is revealed that exon PII-containing transcripts were translated more effectively than exon I.3-containing transcripts. These findings explain why aromatase activity is higher in cells with the PII-containing transcripts than is cells with the I.3-containing transcripts. Our results indicate that aromatase is functionally expressed in human ovarian surface epithelial cells and its expression is regulated at both the transcriptional and translational levels.<\/jats:p>","DOI":"10.1210\/jcem.85.12.7067","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:05:05Z","timestamp":1389200705000},"page":"4889-4899","source":"Crossref","is-referenced-by-count":0,"title":["Regulation of Aromatase Expression in Human Ovarian Surface Epithelial Cells1"],"prefix":"10.1210","volume":"85","author":[{"given":"Tomoharu","family":"Okubo","sequence":"first","affiliation":[{"name":"Division of Immunology (T.O., S.C.), Beckman Research Institute of the City of Hope, Duarte, California 91010"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel C.","family":"Mok","sequence":"additional","affiliation":[{"name":"Laboratory of Gynecology Oncology, Department of Obstetrics, Gynecology and Reproductive Biology (S.C.M.), Brigham and Women\u2019s Hospital, Harvard Medical School. 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