{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T20:00:08Z","timestamp":1722110408355},"reference-count":66,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,4,1]]},"abstract":"<jats:p>Suppression of endometrial matrix metalloproteinases (MMPs) is necessary to maintain tissue stability during the invasive events of implantation and placental development. Several laboratories have shown that inflammatory cytokines, including interleukin-1\u03b1 (IL-1\u03b1), can oppose progesterone suppression of MMPs in the human endometrium. Furthermore, we have recently demonstrated colocalization of epithelial cell IL-1\u03b1 and MMP-7 expression at sites of ectopic pregnancy. The current study extends these findings, revealing a previously unrecognized interrelationship between progesterone and IL-1\u03b1 in regulation of MMP-3. Although IL-1\u03b1 is a potent stimulator of MMP-3 in proliferative phase endometrium in organ culture, we demonstrate that progesterone exposure in vivo reduces IL-1\u03b1 stimulation of MMP-3 in secretory phase tissue. This loss of sensitivity to IL-1\u03b1 was duplicated in isolated stromal cells treated with progesterone in vitro, and IL-1\u03b1 stimulation of MMP-3 returned in a dose-dependent manner with progesterone withdrawal. The antiprogestin, onapristone, partially blocked the ability of progesterone to prevent stimulation of MMP-3 by IL-1\u03b1. These data suggest a novel mechanism by which progesterone may preserve tissue integrity during the establishment and maintenance of pregnancy by limiting stimulation of MMPs by inflammatory cytokines such as IL-1\u03b1.<\/jats:p>","DOI":"10.1210\/jcem.85.4.6502","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:05:32Z","timestamp":1389200732000},"page":"1611-1619","source":"Crossref","is-referenced-by-count":0,"title":["Progesterone Exposure Prevents Matrix Metalloproteinase-3 (MMP-3) Stimulation by Interleukin-1\u03b1 in Human Endometrial Stromal Cells1"],"prefix":"10.1210","volume":"85","author":[{"given":"Nancy R.","family":"Keller","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Pathology and the Women\u2019s Reproductive Health Research Center, Department of Obstetrics and Gynecology, Vanderbilt University School of Medicine, Nashville, Tennessee 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