{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:48Z","timestamp":1791158448383,"version":"4.1.0"},"reference-count":28,"publisher":"The Endocrine Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Leptin is secreted by adipocytes and regulates appetite through interaction with hypothalamic leptin receptors (OB-R). Accumulated evidence shows that leptin is involved in the stimulation of reproductive functions and that local expression of leptin and OB-R in the ovary, oocyte, embryo, and placenta plays a role in early development. To investigate the role of leptin in implantation, we examined the expression of OB-R and leptin in the human endometrium. Northern and Western blot analyses and RT-PCR showed that the long form of OB-R (OB-RL) messenger ribonucleic acid (mRNA) and protein were expressed. In contrast, leptin mRNA or protein was not detected. All of the splice variants of OB-R (OB-RT) and OB-RL transcripts were expressed in 90% and 84% of the cases, respectively. OB-R mRNA expression peaked in the early secretory phase. Decidual tissue of early gestation also expressed OB-RT and OB-RL. Their incidence and abundance were comparable among endometria with benign uterine diseases and disease-free endometria and were not related to a body mass index within the normal range. The present results indicate that OB-R, but not leptin, is expressed in the human endometrium.<\/jats:p>","DOI":"10.1210\/jcem.85.5.6567","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:05:47Z","timestamp":1389200747000},"page":"1946-1950","source":"Crossref","is-referenced-by-count":108,"title":["Expression of Leptin Receptor in Human Endometrium and Fluctuation during the Menstrual Cycle"],"prefix":"10.1210","volume":"85","author":[{"given":"Jo","family":"Kitawaki","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisato","family":"Koshiba","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Ishihara","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izumi","family":"Kusuki","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Tsukamoto","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Honjo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071617471057300_R1","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1038\/372425a0","article-title":"Positional cloning of the mouse obese gene and its human homologue.","volume":"372","author":"Zhang","year":"1994","journal-title":"Nature"},{"key":"2020071617471057300_R2","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1038\/nm1195-1155","article-title":"Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects.","volume":"1","author":"Maffei","year":"1995","journal-title":"Nat Med"},{"key":"2020071617471057300_R3","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1056\/NEJM199602013340503","article-title":"Serum immunoreactive-leptin concentrations in normal-weight and obese humans.","volume":"334","author":"Considine","year":"1996","journal-title":"N Engl J Med"},{"key":"2020071617471057300_R4","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1016\/0092-8674(95)90151-5","article-title":"Identification and expression cloning of a leptin receptor, OB-R.","volume":"83","author":"Tartaglia","year":"1995","journal-title":"Cell"},{"key":"2020071617471057300_R5","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/ng0996-95","article-title":"Leptin activation of Stat3 in the hypothalamus of wild-type and ob\/ob mice but not db\/db mice.","volume":"14","author":"Vaisse","year":"1996","journal-title":"Nat Genet"},{"key":"2020071617471057300_R6","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1038\/nm0596-585","article-title":"Novel B219\/OB receptor isoforms; possible role of leptin in hematopoiesis and reproduction.","volume":"2","author":"Cioffi","year":"1996","journal-title":"Nat Med"},{"key":"2020071617471057300_R7","doi-asserted-by":"crossref","first-page":"3144","DOI":"10.1210\/endo.137.7.8770941","article-title":"Leptin is a metabolic signal to the reproductive system.","volume":"137","author":"Barash","year":"1996","journal-title":"Endocrinology"},{"key":"2020071617471057300_R8","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1038\/ng0396-318","article-title":"Correction of the sterility defect in homozygous obese female mice by treatment with the human recombinant leptin.","volume":"12","author":"Chehab","year":"1996","journal-title":"Nat Genet"},{"key":"2020071617471057300_R9","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1172\/JCI119172","article-title":"Leptin accelerates the onset of puberty in normal female mice.","volume":"99","author":"Ahima","year":"1997","journal-title":"J Clin Invest"},{"key":"2020071617471057300_R10","first-page":"3239","article-title":"Leptin is inversely related to age at menarche in human females.","volume":"82","author":"Matkovic","year":"1997","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071617471057300_R11","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1093\/molehr\/3.6.467","article-title":"The expression of leptin and its receptors in pre-ovulatory human follicles.","volume":"3","author":"Cioffi","year":"1997","journal-title":"Mol Hum Reprod"},{"key":"2020071617471057300_R12","doi-asserted-by":"crossref","first-page":"2226","DOI":"10.1093\/humrep\/12.10.2226","article-title":"A novel mechanism of vascular endothelial growth factor, leptin and transforming growth factor-\u03b22 sequestration in a subpopulation of human ovarian follicle cells.","volume":"12","author":"Antczak","year":"1997","journal-title":"Hum Reprod"},{"key":"2020071617471057300_R13","first-page":"4144","article-title":"Expression of functional leptin receptors in the human ovary.","volume":"82","author":"Karlsson","year":"1997","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071617471057300_R14","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1093\/molehr\/3.12.1067","article-title":"Oocyte influences on early development: the regulatory proteins leptin and STAT3 are polarized in mouse and human oocytes and differentially distributed within the cells of the preimplantation stage embryo.","volume":"3","author":"Antczak","year":"1997","journal-title":"Mol Hum Reprod"},{"key":"2020071617471057300_R15","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1093\/humrep\/14.2.429","article-title":"Temporal and spatial aspects of fragmentation in early human embryos: possible effects on developmental competence and association with the differential elimination of regulatory proteins from polarized domains.","volume":"14","author":"Antczak","year":"1999","journal-title":"Hum Reprod"},{"key":"2020071617471057300_R16","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1038\/nm0997-1029","article-title":"Nonadipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans.","volume":"3","author":"Masuzaki","year":"1997","journal-title":"Nat Med"},{"key":"2020071617471057300_R17","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1210\/endo.138.10.5573","article-title":"Synthesis of leptin in human placenta.","volume":"138","author":"Senaris","year":"1997","journal-title":"Endocrinology"},{"key":"2020071617471057300_R18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1677\/jme.0.0180077","article-title":"Cloning and characterization of a human leptin receptor using a biologically active leptin immunoadhesion.","volume":"18","author":"Luoh","year":"1997","journal-title":"J Mol Endocrinol"},{"key":"2020071617471057300_R19","first-page":"1020","article-title":"Expression of placental leptin and leptin receptor transcripts in early pregnancy and at term.","volume":"92","author":"Henson","year":"1998","journal-title":"Obstet Gynecol"},{"key":"2020071617471057300_R20","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1016\/S0015-0282(99)00424-0","article-title":"Detection of aromatase cytochrome P450 in endometrial biopsy specimens as a diagnostic test for endometriosis.","volume":"72","author":"Kitawaki","year":"1999","journal-title":"Fertil Steril"},{"key":"2020071617471057300_R21","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0015-0282(16)30062-0","article-title":"Dating the endometrial biopsy.","volume":"1","author":"Noyes","year":"1950","journal-title":"Fertil Steril"},{"key":"2020071617471057300_R22","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1095\/biolreprod57.3.514","article-title":"Expression of aromatase cytochrome P450 protein and messenger ribonucleic acid in human endometriotic and adenomyotic tissues but not in normal endometrium.","volume":"57","author":"Kitawaki","year":"1997","journal-title":"Biol Reprod"},{"key":"2020071617471057300_R23","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/S0026-0495(98)90124-X","article-title":"Leptin receptor isoforms expressed in human adipose tissue.","volume":"47","author":"Kielar","year":"1998","journal-title":"Metabolism"},{"key":"2020071617471057300_R24","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1093\/molehr\/5.8.708","article-title":"Leptin directly stimulates aromatase activity in human luteinized granulosa cells.","volume":"5","author":"Kitawaki","year":"1999","journal-title":"Mol Hum Reprod"},{"key":"2020071617471057300_R25","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1006\/bbrc.1999.0365","article-title":"Tyrosine phosphorylation of STAT3 by leptin through leptin receptor in mouse metaphase 2 stage oocyte.","volume":"256","author":"Matsuoka","year":"1999","journal-title":"Biochem Biophys Res Commun"},{"key":"2020071617471057300_R26","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/0002-9378(82)90217-4","article-title":"Sperm phagocytosis by human peritoneal macrophages: a possible cause of infertility in endometriosis.","volume":"144","author":"Muscato","year":"1982","journal-title":"Am J Obstet Gynecol"},{"key":"2020071617471057300_R27","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1016\/S0015-0282(16)60078-X","article-title":"Hormonal profiles and embryo quality in women with severe endometriosis treated by in vitro fertilization and embryo transfer.","volume":"50","author":"Yovich","year":"1988","journal-title":"Fertil Steril"},{"key":"2020071617471057300_R28","first-page":"643","article-title":"Aberrant integrin expression in the endometrium of women with endometriosis.","volume":"79","author":"Lessy","year":"1994","journal-title":"J Clin Endocrinol Metab"}],"container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/85\/5\/1946\/9060084\/jcem1946.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/85\/5\/1946\/9060084\/jcem1946.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T03:37:10Z","timestamp":1594957030000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/85\/5\/1946\/2660553"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5,1]]},"references-count":28,"aliases":["10.1210\/jc.85.5.1946"],"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5,1]]}},"URL":"https:\/\/doi.org\/10.1210\/jcem.85.5.6567","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5,1]]}}}