{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T17:40:44Z","timestamp":1781545244205,"version":"3.54.5"},"reference-count":35,"publisher":"The Endocrine Society","issue":"7","license":[{"start":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T00:00:00Z","timestamp":1590019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K12HD051959"],"award-info":[{"award-number":["K12HD051959"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32DK007529"],"award-info":[{"award-number":["T32DK007529"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32DK007699"],"award-info":[{"award-number":["T32DK007699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pediatric Endocrine Society Clinical Scholar Award"},{"name":"Boston Children\u2019s Hospital Faculty Development Award","award":["R01 HD082314"],"award-info":[{"award-number":["R01 HD082314"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A critical body weight is necessary for pubertal development, an effect mediated in part by leptin. The potential regulation by leptin of Makorin Ring Finger Protein 3 (MKRN3), in which loss-of-function mutations are the most common genetic cause of central precocious puberty, has not been previously explored. In mice, expression of Mkrn3 in the hypothalamic arcuate nucleus is high early in life and declines before the onset of puberty. Therefore, we aimed to explore if leptin contributes to the decrease in hypothalamic Mkrn3 mRNA levels observed in mice during pubertal development. We first used a leptin-deficient (ob\/ob) mouse model. Mkrn3 mRNA levels in the mediobasal hypothalamus (MBH), which includes the arcuate nucleus, and in the preoptic area (POA), both showed a significant decrease with age from postnatal day (PND) 12 to PND30 in ob\/ob mice in both males and females, similar to that observed in wild-type mice. To further explore the effects of leptin on Mkrn3 expression, we exposed prepubertal wild-type mice to high levels of leptin from age PND9-12, which did not result in any significant difference in Mkrn3 expression levels in either the MBH or POA. In summary, regulation of Mkrn3 expression by leptin was not observed in either the MBH or the POA, 2 hypothalamic sites important for pubertal maturation. These data suggest that the decline in Mkrn3 at the onset of puberty may occur independently of leptin and support our hypothesis that MKRN3 is a bona fide controller of puberty initiation.<\/jats:p>","DOI":"10.1210\/jendso\/bvaa059","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T12:38:33Z","timestamp":1590064713000},"source":"Crossref","is-referenced-by-count":13,"title":["The Peripubertal Decline in Makorin Ring Finger Protein 3 Expression is Independent of Leptin Action"],"prefix":"10.1210","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2811-1322","authenticated-orcid":false,"given":"Stephanie A","family":"Roberts","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Boston Children\u2019s Hospital, Boston, Massachusetts"},{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"},{"name":"Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Abreu","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"},{"name":"Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor M","family":"Navarro","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"},{"name":"Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy N","family":"Liang","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline A","family":"Maguire","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Kyeol","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rona S","family":"Carroll","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"},{"name":"Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ursula B","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes and Hypertension, Brigham and Women\u2019s Hospital, Boston, Massachusetts"},{"name":"Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2020,5,21]]},"reference":[{"issue":"8","key":"2020080409503190800_CIT0001","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1007\/s40618-017-0627-9","article-title":"The mystery of puberty initiation: genetics and epigenetics of idiopathic central precocious puberty (ICPP)","volume":"40","author":"Leka-Emiri","year":"2017","journal-title":"J Endocrinol Invest."},{"issue":"4","key":"2020080409503190800_CIT0002","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1097\/MED.0b013e32836318ce","article-title":"Metabolic influences on neuroendocrine regulation of reproduction","volume":"20","author":"Navarro","year":"2013","journal-title":"Curr Opin Endocrinol Diabetes Obes."},{"issue":"1","key":"2020080409503190800_CIT0003","doi-asserted-by":"crossref","first-page":"T1","DOI":"10.1530\/JOE-14-0405","article-title":"20 years of leptin: leptin at 20: an overview","volume":"223","author":"Friedman","year":"2014","journal-title":"J Endocrinol."},{"issue":"9","key":"2020080409503190800_CIT0004","first-page":"2849","article-title":"Serum leptin levels in normal children: relationship to age, gender, body mass index, pituitary-gonadal hormones, and pubertal stage","volume":"82","author":"Garcia-Mayor","year":"1997","journal-title":"J Clin Endocrinol Metab."},{"issue":"5","key":"2020080409503190800_CIT0005","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.1172\/JCI1176","article-title":"Postnatal leptin surge and regulation of circadian rhythm of leptin by feeding. 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