{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T15:17:28Z","timestamp":1776525448361,"version":"3.51.2"},"reference-count":45,"publisher":"The Endocrine Society","issue":"9","license":[{"start":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T00:00:00Z","timestamp":1594857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Grant-in-Aid for Scientific Research","award":["26660252"],"award-info":[{"award-number":["26660252"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>GnRH enhances the expression of annexin A5 (ANXA5) in pituitary gonadotropes, and ANXA5 enhances gonadotropin secretion. However, the impact of ANXA5 regulation on the expression of pituitary hormone genes remains unclear. Here, using quantitative PCR, we demonstrated that ANXA5 deficiency in female mice reduced the expression of Fshb and Gh in their pituitary glands. Transcriptome analysis confirmed a specific increase in Nr4a3 mRNA expression in addition to lower levels of Fshb expression in ANXA5-deficient female pituitary glands. This gene was then found to be a GnRH-inducible immediate early gene, and its increased expression caused protein to accumulate in the nucleus after administration of a GnRH agonist in L\u03b2T2 cells, which are an in vitro pituitary gonadotrope model. The increase in ANXA5 protein levels in L\u03b2T2 cells clearly suppressed Nr4a3 expression. siRNA-mediated inhibition of Nr4a3 expression increased Fshb expression. The results revealed that GnRH stimulates Nr4a3 and Anxa5 sequentially. NR4A3 suppression of Fshb may be necessary for later massive secretion of FSH by GnRH in gonadotropes, and Nr4a3 would be negatively regulated by ANXA5 to increase FSH secretion.<\/jats:p>","DOI":"10.1210\/jendso\/bvaa096","type":"journal-article","created":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T22:22:38Z","timestamp":1594851758000},"source":"Crossref","is-referenced-by-count":10,"title":["Augmentation of Nr4a3 and Suppression of Fshb Expression in the Pituitary Gland of Female Annexin A5 Null Mouse"],"prefix":"10.1210","volume":"4","author":[{"given":"Ryota","family":"Terashima","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomotaka","family":"Saigo","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Titaree","family":"Laoharatchatathanin","sequence":"additional","affiliation":[{"name":"Clinic for Small Domestic Animals and Radiology, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiro","family":"Kurusu","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bent","family":"Brachvogel","sequence":"additional","affiliation":[{"name":"Experimental Neonatology, Department of Pediatrics and Adolescent Medicine, Center for Biochemistry, Medical Faculty, University of Cologne, Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernst","family":"P\u00f6schl","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of East Anglia, Norwich, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-4413","authenticated-orcid":false,"given":"Mitsumori","family":"Kawaminami","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Physiology, School of Veterinary Medicine, Kitasato University, Aomori, Japan"},{"name":"Laboratory of Veterinary Physiology, School of Veterinary Medicine, Okayama University of Science, Imabari, Ehime, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","published-online":{"date-parts":[[2020,7,16]]},"reference":[{"issue":"6","key":"2020091203240585100_CIT0001","doi-asserted-by":"crossref","first-page":"1334","DOI":"10.1016\/S0006-291X(71)80019-0","article-title":"Structure of the porcine LH- and FSH-releasing hormone. 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