{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:15:02Z","timestamp":1792512902750,"version":"4.5.0"},"reference-count":33,"publisher":"The Endocrine Society","issue":"7","license":[{"start":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T00:00:00Z","timestamp":1646784000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"Children\u2019s Hospital Colorado Research Institute Research Scholar Award","award":["K12 HD057022-06)"],"award-info":[{"award-number":["K12 HD057022-06)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Polycystic ovary syndrome (PCOS) is common and diagnosis requires an elevated testosterone. The clinical importance of adrenal 11-oxyandrogens in PCOS is unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>We sought to determine if 11-oxyandrogens 1) better identify PCOS diagnosis compared to testosterone, 2) predict clinical comorbidities of PCOS, and 3) are altered with an combined oral contraceptive pill (COCP) or metformin therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Data from 200 adolescent female participants aged 12 to 21 years, most with obesity, enrolled across 6 studies in pediatric endocrinology were included: 70 non-PCOS controls, 115 untreated PCOS, 9 PCOS\u2005+\u2005obesity treated with COCP, and 6 PCOS\u2005+\u2005obesity treated with metformin. 11-Hydroxyandrostenedione (11-OHA4), 11-hydroxytestosterone (1-OHT), 11-ketotestosterone (11-KT), and testosterone were measured with liquid chromatography\u2013tandem mass spectrometry. Data between 1) untreated PCOS and controls and 2) untreated PCOS and the 2 treatment groups were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Untreated girls with PCOS had higher 11-OHA4 (P\u2005=\u2005.003) and 11-OHT (P\u2005=\u2005.005) compared to controls, but not 11-KT (P\u2005=\u2005.745). Elevated 11-OHA4 remained statistically significant after controlling for obesity. Testosterone better predicted PCOS status compared to 11-oxyandrogens (receiver operating characteristic curve analysis: 11-OHA4 area under the curve [AUC]\u2005=\u20050.620, 11-OHT AUC\u2005=\u20050.638; testosterone AUC\u2005=\u20050.840). Among untreated PCOS patients, all 3 11-oxyandrogens correlated with hirsutism severity. 11-KT (P\u2005=\u2005.039) and testosterone (P\u2005&amp;lt;\u2005.006) were lower in those on COCP treatment compared to untreated PCOS. Metformin treatment had no effect on 11-oxyandrogens, although testosterone was lower (P\u2005=\u2005.01).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Although 11-oxyandrogens do not aid in the diagnosis of PCOS, they relate to excess hair growth. COCP treatment may related to 11-KT; however, further work is needed to determine causality, relationship with metabolic outcomes, and the clinical utility of measuring these androgens in PCOS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/jendso\/bvac037","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T06:05:48Z","timestamp":1646805948000},"source":"Crossref","is-referenced-by-count":21,"title":["11-Oxyandrogens in Adolescents With Polycystic Ovary Syndrome"],"prefix":"10.1210","volume":"6","author":[{"given":"Anya E","family":"Taylor","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meredith A","family":"Ware","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily","family":"Breslow","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5577-8221","authenticated-orcid":false,"given":"Laura","family":"Pyle","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"},{"name":"Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9562-581X","authenticated-orcid":false,"given":"Cameron","family":"Severn","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"},{"name":"Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-3356","authenticated-orcid":false,"given":"Kristen J","family":"Nadeau","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"},{"name":"Center for Women\u2019s Health Research, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3067-7672","authenticated-orcid":false,"given":"Christine L","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0755-1951","authenticated-orcid":false,"given":"Megan M","family":"Kelsey","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"},{"name":"Center for Women\u2019s Health Research, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1593-1695","authenticated-orcid":false,"given":"Melanie","family":"Cree-Green","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Pediatric Endocrinology, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA"},{"name":"Center for Women\u2019s Health Research, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2022,3,9]]},"reference":[{"issue":"3","key":"2022052105443487700_CIT0001","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1210\/jc.2013-3399","article-title":"Hyperandrogenemia predicts metabolic phenotype in polycystic ovary syndrome: the utility of serum androstenedione","volume":"99","author":"O\u2019Reilly","year":"2014","journal-title":"J Clin Endocrinol Metab."},{"issue":"9","key":"2022052105443487700_CIT0002","doi-asserted-by":"crossref","first-page":"1602","DOI":"10.1093\/humrep\/dey256","article-title":"Recommendations from the international evidence-based guideline for the assessment and management of polycystic ovary syndrome","volume":"33","author":"Teede","year":"2018","journal-title":"Hum Reprod."},{"issue":"7","key":"2022052105443487700_CIT0003","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1056\/NEJM199402173300703","article-title":"Hyperandrogenism in polycystic ovary syndrome. Evidence of dysregulation of 11beta-hydroxysteroid dehydrogenase","volume":"330","author":"Rodin","year":"1994","journal-title":"N Engl J Med."},{"issue":"11","key":"2022052105443487700_CIT0004","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1002\/oby.21651","article-title":"Hepatic steatosis is common in adolescents with obesity and PCOS and relates to de novo lipogenesis but not insulin resistance","volume":"24","author":"Cree-Green","year":"2016","journal-title":"Obesity (Silver Spring)."},{"key":"2022052105443487700_CIT0005","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.mce.2016.08.014","article-title":"new dawn for androgens: novel lessons from 11-oxygenated C19 steroids","volume":"441","author":"Pretorius","year":"2017","journal-title":"Mol Cell Endocrinol"},{"issue":"5","key":"2022052105443487700_CIT0006","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1038\/s41574-020-0336-x","article-title":"11-Oxygenated androgens in health and disease","volume":"16","author":"Turcu","year":"2020","journal-title":"Nat Rev Endocrinol."},{"issue":"12","key":"2022052105443487700_CIT0007","first-page":"4589","article-title":"11-Ketotestosterone is the dominant circulating bioactive androgen during normal and premature adrenarche","volume":"103","author":"Rege","year":"2018","journal-title":"J Clin Endocrinol Metab."},{"issue":"3","key":"2022052105443487700_CIT0008","doi-asserted-by":"crossref","first-page":"840","DOI":"10.1210\/jc.2016-3285","article-title":"11-Oxygenated C19 steroids are the predominant androgens in polycystic ovary syndrome","volume":"102","author":"O\u2019Reilly","year":"2017","journal-title":"J Clin Endocrinol Metab."},{"issue":"11","key":"2022052105443487700_CIT0009","doi-asserted-by":"crossref","first-page":"e3903","DOI":"10.1210\/clinem\/dgaa532","article-title":"11-Oxygenated C19 steroids do not distinguish the hyperandrogenic phenotype of PCOS daughters from girls with obesity","volume":"105","author":"Torchen","year":"2020","journal-title":"J Clin Endocrinol Metab."},{"issue":"7","key":"2022052105443487700_CIT0010","first-page":"78","article-title":"Steroid mass spectrometry for the diagnosis of PCOS","volume":"7","author":"Keevil","year":"2019","journal-title":"Med Sci (Basel)."},{"issue":"10","key":"2022052105443487700_CIT0011","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1507\/endocrj.EJ18-0212","article-title":"11-Oxygenated C19 steroids as circulating androgens in women with polycystic ovary syndrome","volume":"65","author":"Yoshida","year":"2018","journal-title":"Endocr J."},{"issue":"4","key":"2022052105443487700_CIT0012","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S0015-0282(16)55317-5","article-title":"11 Beta-hydroxyandrostenedione in plasma, follicular fluid, and granulosa cells of women with normal and polycystic ovaries","volume":"58","author":"Owen","year":"1992","journal-title":"Fertil Steril."},{"issue":"7","key":"2022052105443487700_CIT0013","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1210\/js.2017-00192","article-title":"Insulin resistance, hyperinsulinemia, and mitochondria dysfunction in nonobese girls with polycystic ovarian syndrome","volume":"1","author":"Cree-Green","year":"2017","journal-title":"J Endocr Soc."},{"issue":"4","key":"2022052105443487700_CIT0014","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1111\/cen.14062","article-title":"Clinical prediction score of nonalcoholic fatty liver disease in adolescent girls with polycystic ovary syndrome (PCOS-HS index)","volume":"91","author":"Carreau","year":"2019","journal-title":"Clin Endocrinol (Oxf)."},{"issue":"1","key":"2022052105443487700_CIT0015","doi-asserted-by":"crossref","first-page":"R134","DOI":"10.1152\/ajpregu.00047.2019","article-title":"A simple method to monitor hepatic gluconeogenesis and triglyceride synthesis following oral sugar tolerance test in obese adolescents","volume":"317","author":"Carreau","year":"2019","journal-title":"Am J Physiol Regul Integr Comp Physiol."},{"issue":"8","key":"2022052105443487700_CIT0016","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1111\/pedi.12132","article-title":"Hemoglobin A1c assay variations and implications for diabetes screening in obese youth","volume":"15","author":"Chan","year":"2014","journal-title":"Pediatr Diabetes."},{"issue":"3","key":"2022052105443487700_CIT0017","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/pedi.12258","article-title":"Screening for type 2 diabetes and prediabetes in obese youth: evaluating alternate markers of glycemia\u20141,5-anhydroglucitol, fructosamine, and glycated albumin","volume":"17","author":"Chan","year":"2016","journal-title":"Pediatr Diabetes."},{"key":"2022052105443487700_CIT0018","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.jpeds.2020.10.044","article-title":"Puberty is associated with a rising hemoglobin A1c, even in youth with normal weight","volume":"230","author":"Kelsey","year":"2021","journal-title":"J Pediatr."},{"issue":"6","key":"2022052105443487700_CIT0019","doi-asserted-by":"crossref","first-page":"e2134","DOI":"10.1210\/clinem\/dgz263","article-title":"Obese adolescents with PCOS have altered biodiversity and relative abundance in gastrointestinal microbiota","volume":"105","author":"Jobira","year":"2020","journal-title":"J Clin Endocrinol Metab."},{"issue":"9","key":"2022052105443487700_CIT0020","doi-asserted-by":"crossref","first-page":"1462","DOI":"10.2337\/diacare.22.9.1462","article-title":"Insulin sensitivity indices obtained from oral glucose tolerance testing: comparison with the euglycemic insulin clamp","volume":"22","author":"Matsuda","year":"1999","journal-title":"Diabetes Care."},{"key":"2022052105443487700_CIT0021","doi-asserted-by":"crossref","first-page":"100078","DOI":"10.1016\/j.metop.2021.100078","article-title":"Oral minimal model-based estimates of insulin sensitivity in obese youth depend on oral glucose tolerance test protocol duration","volume":"9","author":"Bartlette","year":"2021","journal-title":"Metabol Open."},{"issue":"6","key":"2022052105443487700_CIT0022","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1016\/S0015-0282(16)54006-0","article-title":"11 Beta-hydroxyandrostenedione: a marker of adrenal function in hirsutism","volume":"54","author":"Hudson","year":"1990","journal-title":"Fertil Steril."},{"issue":"3-8","key":"2022052105443487700_CIT0023","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/0960-0760(92)90441-K","article-title":"Studies to confirm the source of 11 beta-hydroxyandrostenedione","volume":"41","author":"Holownia","year":"1992","journal-title":"J Steroid Biochem Mol Biol."},{"issue":"8","key":"2022052105443487700_CIT0024","doi-asserted-by":"crossref","first-page":"e2921","DOI":"10.1210\/clinem\/dgaa343","article-title":"Sex differences in 11-oxygenated androgen patterns across adulthood","volume":"105","author":"Davio","year":"2020","journal-title":"J Clin Endocrinol Metab."},{"issue":"5","key":"2022052105443487700_CIT0025","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1210\/js.2017-00013","article-title":"Increased adrenal androgens in overweight peripubertal girls","volume":"1","author":"Burt Solorzano","year":"2017","journal-title":"J Endocr Soc."},{"issue":"4","key":"2022052105443487700_CIT0026","doi-asserted-by":"crossref","first-page":"e93805","DOI":"10.1371\/journal.pone.0093805","article-title":"Simultaneous measurement of thirteen steroid hormones in women with polycystic ovary syndrome and control women using liquid chromatography-tandem mass spectrometry","volume":"9","author":"Keefe","year":"2014","journal-title":"PLoS One."},{"key":"2022052105443487700_CIT0027","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.cca.2017.06.009","article-title":"New biomarkers for diagnosis and management of polycystic ovary syndrome","volume":"471","author":"Karakas","year":"2017","journal-title":"Clin Chim Acta."},{"issue":"2","key":"2022052105443487700_CIT0028","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1016\/j.fertnstert.2007.11.069","article-title":"Possible metformin effect on adrenal androgens during pretreatment and IVF cycle in women with polycystic ovary syndrome","volume":"91","author":"Kj\u00f8tr\u00f8d","year":"2009","journal-title":"Fertil Steril."},{"key":"2022052105443487700_CIT0029","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.jsbmb.2014.06.003","article-title":"DHEA, DHEAS and PCOS","volume":"145","author":"Goodarzi","year":"2015","journal-title":"J Steroid Biochem Mol Biol."},{"issue":"36","key":"2022052105443487700_CIT0030","doi-asserted-by":"crossref","first-page":"5588","DOI":"10.2174\/1381612822666160720150625","article-title":"Adrenal hyperandrogenism and polycystic ovary syndrome","volume":"22","author":"Luque-Ram\u00edrez","year":"2016","journal-title":"Curr Pharm Des."},{"issue":"3","key":"2022052105443487700_CIT0031","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1210\/jc.2014-2569","article-title":"Adrenal androgen excess and body mass index in polycystic ovary syndrome","volume":"100","author":"Moran","year":"2015","journal-title":"J Clin Endocrinol Metab."},{"issue":"4","key":"2022052105443487700_CIT0032","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.steroids.2011.12.007","article-title":"Neuroendocrine dysfunction in polycystic ovary syndrome","volume":"77","author":"Burt Solorzano","year":"2012","journal-title":"Steroids."},{"issue":"3","key":"2022052105443487700_CIT0033","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/j.fertnstert.2014.12.105","article-title":"Influence of adrenal hyperandrogenism on the clinical and metabolic phenotype of women with polycystic ovary syndrome","volume":"103","author":"Alpa\u00f1\u00e9s","year":"2015","journal-title":"Fertil Steril."}],"container-title":["Journal of the Endocrine Society"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jes\/advance-article-pdf\/doi\/10.1210\/jendso\/bvac037\/42778798\/bvac037.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jes\/advance-article-pdf\/doi\/10.1210\/jendso\/bvac037\/43779195\/bvac037.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jes\/advance-article-pdf\/doi\/10.1210\/jendso\/bvac037\/43779195\/bvac037.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T01:45:22Z","timestamp":1653097522000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jes\/article\/doi\/10.1210\/jendso\/bvac037\/6545258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,9]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7,1]]}},"URL":"https:\/\/doi.org\/10.1210\/jendso\/bvac037","relation":{},"ISSN":["2472-1972"],"issn-type":[{"value":"2472-1972","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,7,1]]},"published":{"date-parts":[[2022,3,9]]},"article-number":"bvac037"}}