{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:46:55Z","timestamp":1787021215866,"version":"build-2736575974"},"reference-count":23,"publisher":"The Endocrine Society","issue":"6","funder":[{"DOI":"10.13039\/100030692","name":"Intramural Research Program","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100030692","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Context<\/jats:title>\n                    <jats:p>Spatial transcriptomic (ST) analysis of tumors provides a novel approach to studying gene expression along with the localization of tumor cells in their environment to uncover spatial interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>We present ST analysis of corticotroph pituitary neuroendocrine tumors (PitNETs) from formalin-fixed, paraffin-embedded tissues. ST data were compared to immunohistochemistry results. Gene expression profiles were reviewed for cluster annotations, and differentially expressed genes were used for pathway analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Seven tumors were used for ST analysis. In situ annotation of tumor tissue was inferred from the gene expression profiles and was in concordance with the annotation made by a pathologist. Furthermore, relative gene expression in the tumor corresponded to common protein staining used in the evaluation of PitNETs, such as reticulin and Ki-67 index. Finally, we identified intratumor heterogeneity; clusters within the same tumor may present with different transcriptomic profiles, unveiling potential intratumor cell variability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Together, our results provide the first attempt to clarify the spatial cell profile in PitNETs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1210\/jendso\/bvae064","type":"journal-article","created":{"date-parts":[[2024,4,3]],"date-time":"2024-04-03T06:27:37Z","timestamp":1712125657000},"source":"Crossref","is-referenced-by-count":4,"title":["Spatial Transcriptomic Analysis of Pituitary Corticotroph Tumors"],"prefix":"10.1210","volume":"8","author":[{"given":"Jeremie Oliver","family":"Pi\u00f1a","sequence":"first","affiliation":[{"name":"Section on Craniofacial Genetic Disorders , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"},{"name":"National Institute of Child Health, and Human Development, National Institutes of Health , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio R","family":"Faucz","sequence":"additional","affiliation":[{"name":"Molecular Genomics Core , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"},{"name":"National Institute of Child Health and Human Development, National Institutes of Health , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cameron","family":"Padilla","sequence":"additional","affiliation":[{"name":"Molecular Genomics Core , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"},{"name":"National Institute of Child Health and Human Development, National Institutes of Health , Eunice Kennedy Shriver , Bethesda, MD 20892 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0020-237X","authenticated-orcid":false,"given":"Charalampos S","family":"Floudas","sequence":"additional","affiliation":[{"name":"Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health , Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9349-4756","authenticated-orcid":false,"given":"Prashant","family":"Chittiboina","sequence":"additional","affiliation":[{"name":"Neurosurgery Unit for Pituitary and Inheritable Diseases, National Institute of Neurological Disorders and Stroke, National Institutes of Health , Bethesda, MD 20892 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Quezado","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology, Center for Cancer Research, National Institutes of Health , Bethesda, 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