{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:44:21Z","timestamp":1782409461248,"version":"3.54.5"},"reference-count":33,"publisher":"The Endocrine Society","issue":"6","license":[{"start":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T00:00:00Z","timestamp":1713571200000},"content-version":"vor","delay-in-days":14,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tohoku University Hospital"},{"name":"Fujirebio Inc"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,6]]},"abstract":"Abstract<\/jats:title>\n \n Context<\/jats:title>\n Detecting patients with surgically curable aldosterone-producing adenoma (APA) among hypertensive individuals is clinically pivotal. Liquid chromatography\u2013tandem mass spectrometry (LC-MS\/MS) is the ideal method of measuring plasma aldosterone concentration (PAC) because of the inaccuracy of conventional chemiluminescent enzyme immunoassay (CLEIA). However, LC-MS\/MS is expensive and requires expertise. We have developed a novel noncompetitive CLEIA (NC-CLEIA) for measuring PAC in 30 minutes.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n This work aimed to validate NC-CLEIA PAC measurements by comparing them with LC-MS\/MS measurements and determining screening cutoffs for both measurements detecting APA.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We retrospectively measured PAC using LC-MS\/MS and NC-CLEIA in 133 patients with APA, 100 with bilateral hyperaldosteronism, and 111 with essential hypertension to explore the accuracy of NC-CLEIA PAC measurements by comparing with LC-MS\/MS measurements and determined the cutoffs for detecting APA.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Passing-Bablok analysis revealed that the values by NC-CLEIA (the regression slope, intercept, and correlation coefficient were 0.962, \u22120.043, and 0.994, respectively) were significantly correlated and equivalent to those by LC-MS\/MS. Bland-Altman plot analysis of NC-CLEIA and LC-MS\/MS also demonstrated smaller systemic errors (a bias of \u22120.348 ng\/dL with limits of agreement of \u22124.390 and 3.694 within a 95% CI) in NC-CLEIA than LC-MS\/MS. The receiver operating characteristic analysis demonstrated that cutoff values for aldosterone\/renin activity ratio obtained by LC-MS\/MS and NC-CLEIA were 31.2 and 31.5 (ng\/dL per ng\/mL\/hour), with a sensitivity of 91.0% and 90.2% and specificity of 75.4% and 76.8%, respectively, to differentiate APA from non-APA.<\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n This newly developed NC-CLEIA for measuring PAC could serve as a clinically reliable alternative to LC-MS\/MS.<\/jats:p>\n <\/jats:sec>","DOI":"10.1210\/jendso\/bvae080","type":"journal-article","created":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T21:06:02Z","timestamp":1713647162000},"source":"Crossref","is-referenced-by-count":12,"title":["Screening Cutoff Values for the Detection of Aldosterone-Producing Adenoma by LC-MS\/MS and a Novel Noncompetitive CLEIA"],"prefix":"10.1210","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1873-7388","authenticated-orcid":false,"given":"Yoshikiyo","family":"Ono","sequence":"first","affiliation":[{"name":"Department of Diabetes, Metabolism and Endocrinology, Tohoku University Hospital , Sendai, Miyagi 980-8574 , Japan"},{"name":"Division of Nephrology, Rheumatology and Endocrinology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1546-6949","authenticated-orcid":false,"given":"Yuta","family":"Tezuka","sequence":"additional","affiliation":[{"name":"Department of Diabetes, Metabolism and Endocrinology, Tohoku University Hospital , Sendai, Miyagi 980-8574 , Japan"},{"name":"Division of Nephrology, Rheumatology and Endocrinology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7422-2659","authenticated-orcid":false,"given":"Kei","family":"Omata","sequence":"additional","affiliation":[{"name":"Department of Diabetes, Metabolism and Endocrinology, Tohoku University Hospital , Sendai, Miyagi 980-8574 , Japan"},{"name":"Division of Nephrology, Rheumatology and Endocrinology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Morimoto","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Rheumatology and Endocrinology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9976-2632","authenticated-orcid":false,"given":"Yuto","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7101-7382","authenticated-orcid":false,"given":"Sota","family":"Oguro","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0931-9942","authenticated-orcid":false,"given":"Kei","family":"Takase","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7224-1100","authenticated-orcid":false,"given":"Akihiro","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Urology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsunari","family":"Yoshimi","sequence":"additional","affiliation":[{"name":"Research and Development Division, Fujirebio Inc , Hachioji, Tokyo 192-0031 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Kojima","sequence":"additional","affiliation":[{"name":"Research and Development Division, Fujirebio Inc , Hachioji, Tokyo 192-0031 , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5092-3626","authenticated-orcid":false,"given":"Sadayoshi","family":"Ito","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Rheumatology and Endocrinology, Tohoku University Graduate School of Medicine , Sendai, Miyagi 980-8575 , 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