{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:21:22Z","timestamp":1791328882892,"version":"4.3.1"},"reference-count":60,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Molecular mechanisms underlying mineralocorticoid receptor (MR)-mediated gene expression are not fully understood but seem to largely depend upon interactions with specific coregulators. To identify novel human MR (hMR) molecular partners, yeast two-hybrid screenings performed using the N-terminal domain as bait, allowed us to isolate protein inhibitor of activated signal transducer and activator of transcription (PIAS)1 and PIASx\u03b2, described as SUMO (small ubiquitin-related modifier) E3-ligases. Specific interaction between PIAS1 and hMR was confirmed by glutathione-S-transferase pull-down experiments and N-terminal subdomains responsible for physical contacts were delineated. Transient transfections demonstrated that PIAS1 is a corepressor of aldosterone-activated MR transactivation but has no significant effect on human glucocorticoid receptor transactivation. The agonist or antagonist nature of the bound ligand also determines PIAS1 corepressive action. We provided evidence that PIAS1 conjugated SUMO-1 to hMR both in vitro and in vivo. Deciphering the unique sumoylation pattern of hMR, which possesses five consensus SUMO-1 binding sites, by combinatorial lysine substitutions, revealed a major impact of sumoylation on hMR properties. Using a murine mammary tumor virus promoter, PIAS1 action was independent of sumoylation whereas with glucocorticoid response element promoter, PIAS1 corepressive action depended on hMR sumoylation status. Taken together, our results identify a novel function for PIAS1 which interacts with the N-terminal domain of hMR and represses its ligand-dependent transcriptional activity, at least in part, through SUMO modifications.<\/jats:p>","DOI":"10.1210\/me.2003-0299","type":"journal-article","created":{"date-parts":[[2003,9,22]],"date-time":"2003-09-22T21:33:15Z","timestamp":1064266395000},"page":"2529-2542","source":"Crossref","is-referenced-by-count":112,"title":["Protein Inhibitor of Activated Signal Transducer and Activator of Transcription 1 Interacts with the N-Terminal Domain of Mineralocorticoid Receptor and Represses Its Transcriptional Activity: Implication of Small Ubiquitin-Related Modifier 1 Modification"],"prefix":"10.1210","volume":"17","author":[{"given":"Laurent","family":"Pascual-Le Tallec","sequence":"first","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unite\u0301 (U) 478 (L.P.-L.T., S.V., M.-C.Z., M.L.), 75870 Paris cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Kirsh","sequence":"additional","affiliation":[{"name":"INSERM U579 (O.K., A.D.), Institut Pasteur, 75724 Paris cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Christine","family":"Lecomte","sequence":"additional","affiliation":[{"name":"INSERM U409 (M.-C.L.), Institut Fe\u0301de\u0301ratif de Recherche Claude Bernard, Faculte\u0301 de Me\u0301decine Xavier Bichat, 75870 Paris cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Say","family":"Viengchareun","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unite\u0301 (U) 478 (L.P.-L.T., S.V., M.-C.Z., M.L.), 75870 Paris cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Christina","family":"Zennaro","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unite\u0301 (U) 478 (L.P.-L.T., S.V., M.-C.Z., M.L.), 75870 Paris cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Dejean","sequence":"additional","affiliation":[{"name":"INSERM U579 (O.K., A.D.), Institut Pasteur, 75724 Paris cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Lombe\u0300s","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) Unite\u0301 (U) 478 (L.P.-L.T., S.V., M.-C.Z., M.L.), 75870 Paris cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071614262106000_R1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0092-8674(00)80726-6","article-title":"Nuclear Receptor Nomenclature Committee","volume":"97","year":"1999","journal-title":"Cell"},{"key":"2020071614262106000_R2","first-page":"S49","article-title":"Regulation of sodium transport by steroid hormones.","volume":"65","author":"Bonvalet","year":"1998","journal-title":"Kidney Int Suppl"},{"key":"2020071614262106000_R3","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0896-6273(88)90136-5","article-title":"The neuronal mineralocorticoid receptor as a mediator of glucocorticoid response.","volume":"1","author":"Arriza","year":"1988","journal-title":"Neuron"},{"key":"2020071614262106000_R4","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1161\/01.CIR.92.2.175","article-title":"Prerequisite for cardiac aldosterone action. Mineralocorticoid receptor and 11\u03b2-hydroxysteroid dehydrogenase in the human heart.","volume":"92","author":"Lombes","year":"1995","journal-title":"Circulation"},{"key":"2020071614262106000_R5","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1126\/science.2845584","article-title":"Mineralocorticoid action: target tissue specificity is enzyme, not receptor, mediated.","volume":"242","author":"Funder","year":"1988","journal-title":"Science"},{"key":"2020071614262106000_R6","doi-asserted-by":"crossref","first-page":"F181","DOI":"10.1152\/ajprenal.2001.280.2.F181","article-title":"Multiple aspects of mineralocorticoid selectivity.","volume":"280","author":"Farman","year":"2001","journal-title":"Am J Physiol Renal Physiol"},{"key":"2020071614262106000_R7","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1210\/endo.135.3.8070376","article-title":"The mineralocorticoid receptor discriminates aldosterone from glucocorticoids independently of the 11\u03b2-hydroxysteroid dehydrogenase.","volume":"135","author":"Lombes","year":"1994","journal-title":"Endocrinology"},{"key":"2020071614262106000_R8","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1042\/bj3150421","article-title":"Ligand-induced conformational change in the human mineralocorticoid receptor occurs within its hetero-oligomeric structure.","volume":"315","author":"Couette","year":"1996","journal-title":"Biochem J"},{"key":"2020071614262106000_R9","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.bi.63.070194.002315","article-title":"Molecular mechanisms of action of steroid\/thyroid receptor superfamily members.","volume":"63","author":"Tsai","year":"1994","journal-title":"Annu Rev Biochem"},{"key":"2020071614262106000_R10","doi-asserted-by":"crossref","first-page":"21016","DOI":"10.1074\/jbc.270.36.21016","article-title":"Human mineralocorticoid receptor genomic structure and identification of expressed isoforms.","volume":"270","author":"Zennaro","year":"1995","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R11","first-page":"321","article-title":"Nuclear receptor coregulators: cellular and molecular biology.","volume":"20","author":"McKenna","year":"1999","journal-title":"Endocr Rev"},{"key":"2020071614262106000_R12","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0960-0760(98)00144-7","article-title":"Nuclear receptor coactivators: multiple enzymes, multiple complexes, multiple functions.","volume":"69","author":"McKenna","year":"1999","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071614262106000_R13","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1038\/42750","article-title":"A signature motif in transcriptional co-activators mediates binding to nuclear receptors.","volume":"387","author":"Heery","year":"1997","journal-title":"Nature"},{"key":"2020071614262106000_R14","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1002\/j.1460-2075.1996.tb00736.x","article-title":"TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors.","volume":"15","author":"Voegel","year":"1996","journal-title":"EMBO J"},{"key":"2020071614262106000_R15","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1128\/MCB.17.5.2735","article-title":"GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors.","volume":"17","author":"Hong","year":"1997","journal-title":"Mol Cell Biol"},{"key":"2020071614262106000_R16","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1210\/mend.12.2.0065","article-title":"Nuclear receptor-binding sites of coactivators glucocorticoid receptor interacting protein 1 (GRIP1) and steroid receptor coactivator 1 (SRC-1): multiple motifs with different binding specificities.","volume":"12","author":"Ding","year":"1998","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R17","doi-asserted-by":"crossref","first-page":"5380","DOI":"10.1093\/emboj\/18.19.5380","article-title":"Differential regulation of glucocorticoid receptor transcriptional activation via AF-1-associated proteins.","volume":"18","author":"Hittelman","year":"1999","journal-title":"EMBO J"},{"key":"2020071614262106000_R18","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0092-8674(00)80711-4","article-title":"A steroid receptor coactivator, SRA, functions as an RNA and is present in an SRC-1 complex.","volume":"97","author":"Lanz","year":"1999","journal-title":"Cell"},{"key":"2020071614262106000_R19","doi-asserted-by":"crossref","first-page":"2007","DOI":"10.1091\/mbc.11.6.2007","article-title":"Interaction of the \u03c42 transcriptional activation domain of glucocorticoid receptor with a novel steroid receptor coactivator, Hic-5, which localizes to both focal adhesions and the nuclear matrix.","volume":"11","author":"Yang","year":"2000","journal-title":"Mol Biol Cell"},{"key":"2020071614262106000_R20","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1210\/mend.14.8.0502","article-title":"Crucial role of the H11\u2013H12 loop in stabilizing the active conformation of the human mineralocorticoid receptor.","volume":"14","author":"Hellal-Levy","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R21","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1210\/mend.15.9.0689","article-title":"A new human MR splice variant is a ligand-independent transactivator modulating corticosteroid action.","volume":"15","author":"Zennaro","year":"2001","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R22","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1210\/mend.14.6.0467","article-title":"Characterization of transactivational property and coactivator mediation of rat mineralocorticoid receptor activation function-1 (AF-1).","volume":"14","author":"Fuse","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R23","doi-asserted-by":"crossref","first-page":"3698","DOI":"10.1128\/MCB.22.11.3698-3706.2002","article-title":"Ligand-selective potentiation of rat mineralocorticoid receptor activation function 1 by a CBP-containing histone acetyltransferase complex.","volume":"22","author":"Kitagawa","year":"2002","journal-title":"Mol Cell Biol"},{"key":"2020071614262106000_R24","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.1101\/gad.955501","article-title":"A new RING for SUMO: wrestling transcriptional responses into nuclear bodies with PIAS family E3 SUMO ligases.","volume":"15","author":"Jackson","year":"2001","journal-title":"Genes Dev"},{"key":"2020071614262106000_R25","doi-asserted-by":"crossref","first-page":"24439","DOI":"10.1074\/jbc.273.38.24439","article-title":"Reconstitution of the N-terminal transcription activation function of human mineralocorticoid receptor in a defective human glucocorticoid receptor.","volume":"273","author":"Govindan","year":"1998","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R26","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1038\/47069","article-title":"The CoRNR motif controls the recruitment of corepressors by nuclear hormone receptors.","volume":"402","author":"Hu","year":"1999","journal-title":"Nature"},{"key":"2020071614262106000_R27","doi-asserted-by":"crossref","first-page":"6040","DOI":"10.1128\/MCB.20.16.6040-6050.2000","article-title":"A common motif within the negative regulatory regions of multiple factors inhibits their transcriptional synergy.","volume":"20","author":"Iniguez-Lluhi","year":"2000","journal-title":"Mol Cell Biol"},{"key":"2020071614262106000_R28","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0014-5793(97)01305-7","article-title":"Ubch9 conjugates SUMO but not ubiquitin.","volume":"417","author":"Desterro","year":"1997","journal-title":"FEBS Lett"},{"key":"2020071614262106000_R29","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/0092-8674(88)90145-6","article-title":"Multiple and cooperative trans-activation domains of the human glucocorticoid receptor.","volume":"55","author":"Hollenberg","year":"1988","journal-title":"Cell"},{"key":"2020071614262106000_R30","doi-asserted-by":"crossref","first-page":"5455","DOI":"10.1074\/jbc.273.10.5455","article-title":"An N-terminal inhibitory function, IF, suppresses transcription by the A-isoform but not the B-isoform of human progesterone receptors.","volume":"273","author":"Hovland","year":"1998","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R31","doi-asserted-by":"crossref","first-page":"7341","DOI":"10.1074\/jbc.270.13.7341","article-title":"Identification of two transcription activation units in the N-terminal domain of the human androgen receptor.","volume":"270","author":"Jenster","year":"1995","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R32","doi-asserted-by":"crossref","first-page":"26772","DOI":"10.1074\/jbc.271.43.26772","article-title":"Delineation of two distinct type 1 activation functions in the androgen receptor amino-terminal domain.","volume":"271","author":"Chamberlain","year":"1996","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R33","doi-asserted-by":"crossref","first-page":"10626","DOI":"10.1073\/pnas.95.18.10626","article-title":"Inhibition of Stat1-mediated gene activation by PIAS1.","volume":"95","author":"Liu","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071614262106000_R34","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1210\/mend.14.1.0408","article-title":"Protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1) is a nuclear receptor coregulator expressed in human testis.","volume":"14","author":"Tan","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R35","doi-asserted-by":"crossref","first-page":"3700","DOI":"10.1074\/jbc.274.6.3700","article-title":"A testis-specific androgen receptor coregulator that belongs to a novel family of nuclear proteins.","volume":"274","author":"Moilanen","year":"1999","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R36","doi-asserted-by":"crossref","first-page":"1986","DOI":"10.1210\/mend.14.12.0569","article-title":"ARIP3 (androgen receptor-interacting protein 3) and other PIAS (protein inhibitor of activated STAT) proteins differ in their ability to modulate steroid receptor-dependent transcriptional activation.","volume":"14","author":"Kotaja","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R37","doi-asserted-by":"crossref","first-page":"16993","DOI":"10.1074\/jbc.M109217200","article-title":"Protein inhibitors of activated STAT resemble scaffold attachment factors and function as interacting nuclear receptor coregulators.","volume":"277","author":"Tan","year":"2002","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R38","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1093\/emboj\/17.12.3317","article-title":"Antagonism in the human mineralocorticoid receptor.","volume":"17","author":"Fagart","year":"1998","journal-title":"EMBO J"},{"key":"2020071614262106000_R39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1210\/me.2002-0258","article-title":"Transactivation functions of the N-terminal domains of nuclear hormone receptors: protein folding and coactivator interactions.","volume":"17","author":"Kumar","year":"2003","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R40","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0303-7207(02)00413-6","article-title":"Interdomain interactions in the mineralocorticoid receptor.","volume":"200","author":"Rogerson","year":"2003","journal-title":"Mol Cell Endocrinol"},{"key":"2020071614262106000_R41","doi-asserted-by":"crossref","first-page":"22986","DOI":"10.1074\/jbc.M002807200","article-title":"FXXLF and WXXLF sequences mediate the NH2-terminal interaction with the ligand binding domain of the androgen receptor.","volume":"275","author":"He","year":"2000","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R42","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1210\/mend.15.6.0647","article-title":"Disrupted amino- and carboxyl-terminal interactions of the androgen receptor are linked to androgen insensitivity.","volume":"15","author":"Thompson","year":"2001","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R43","doi-asserted-by":"crossref","first-page":"1976","DOI":"10.1210\/mend.14.12.0566","article-title":"The nuclear receptor corepressor (N-CoR) contains three isoleucine motifs (I\/LXXII) that serve as receptor interaction domains (IDs).","volume":"14","author":"Webb","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R44","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1210\/mend.15.7.0669","article-title":"The specificity of interactions between nuclear hormone receptors and corepressors is mediated by distinct amino acid sequences within the interacting domains.","volume":"15","author":"Cohen","year":"2001","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R45","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1210\/me.2002-0310","article-title":"Determination of nuclear receptor corepressor interactions with the thyroid hormone receptor.","volume":"17","author":"Makowski","year":"2003","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R46","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/S1097-2765(01)00349-5","article-title":"Involvement of PIAS1 in the sumoylation of tumor suppressor p53.","volume":"8","author":"Kahyo","year":"2001","journal-title":"Mol Cell"},{"key":"2020071614262106000_R47","doi-asserted-by":"crossref","first-page":"2872","DOI":"10.1073\/pnas.052559499","article-title":"Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity.","volume":"99","author":"Schmidt","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071614262106000_R48","doi-asserted-by":"crossref","first-page":"3088","DOI":"10.1101\/gad.944801","article-title":"PIASy, a nuclear matrix-associated SUMO E3 ligase, represses LEF1 activity by sequestration into nuclear bodies.","volume":"15","author":"Sachdev","year":"2001","journal-title":"Genes Dev"},{"key":"2020071614262106000_R49","doi-asserted-by":"crossref","first-page":"3314","DOI":"10.1128\/MCB.21.10.3314-3324.2001","article-title":"Common properties of nuclear body protein SP100 and TIF1\u03b1 chromatin factor: role of SUMO modification.","volume":"21","author":"Seeler","year":"2001","journal-title":"Mol Cell Biol"},{"key":"2020071614262106000_R50","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S0014-5793(02)03154-X","article-title":"PIAS3 (protein inhibitor of activated STAT-3) modulates the transcriptional activation mediated by the nuclear receptor coactivator TIF2.","volume":"526","author":"Jimenez-Lara","year":"2002","journal-title":"FEBS Lett"},{"key":"2020071614262106000_R51","doi-asserted-by":"crossref","first-page":"30283","DOI":"10.1074\/jbc.M204768200","article-title":"The nuclear receptor interaction domain of GRIP1 is modulated by covalent attachment of SUMO-1.","volume":"277","author":"Kotaja","year":"2002","journal-title":"J Biol Chem"},{"key":"2020071614262106000_R52","doi-asserted-by":"crossref","first-page":"14145","DOI":"10.1073\/pnas.97.26.14145","article-title":"Covalent modification of the androgen receptor by small ubiquitin-like modifier 1 (SUMO-1).","volume":"97","author":"Poukka","year":"2000","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071614262106000_R53","first-page":"1399","article-title":"Steroid receptor transcriptional synergy is potentiated by disruption of the DNA-binding domain dimer interface.","volume":"10","author":"Liu","year":"1996","journal-title":"Mol Endocrinol"},{"key":"2020071614262106000_R54","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1042\/bj2920577","article-title":"Characterization of the interaction of the human mineralocorticosteroid receptor with hormone response elements.","volume":"292","author":"Lombes","year":"1993","journal-title":"Biochem J"},{"key":"2020071614262106000_R55","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1126\/science.3037703","article-title":"Cloning of human mineralocorticoid receptor complementary DNA: structural and functional kinship with the glucocorticoid receptor.","volume":"237","author":"Arriza","year":"1987","journal-title":"Science"},{"key":"2020071614262106000_R56","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1210\/endo.143.5.8821","article-title":"Cyclosporine a and FK506 inhibit transcriptional activity of the human mineralocorticoid receptor: a cell-based model to investigate partial aldosterone resistance in kidney transplantation.","volume":"143","author":"Deppe","year":"2002","journal-title":"Endocrinology"},{"key":"2020071614262106000_R57","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1128\/MCB.22.10.3527-3536.2002","article-title":"Tyrosine phosphorylation regulates \u03b1 II spectrin cleavage by calpain.","volume":"22","author":"Nicolas","year":"2002","journal-title":"Mol Cell Biol"},{"key":"2020071614262106000_R58","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1002\/jcp.1041410128","article-title":"Maintenance of proximal and distal cell functions in SV40-transformed tubular cell lines derived from rabbit kidney cortex.","volume":"141","author":"Vandewalle","year":"1989","journal-title":"J Cell Physiol"},{"key":"2020071614262106000_R59","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.1038\/ki.1995.186","article-title":"Role of adenosine on glucagon-induced cAMP in a human cortical collecting duct cell line.","volume":"47","author":"Prie","year":"1995","journal-title":"Kidney Int"},{"key":"2020071614262106000_R60","first-page":"1549","article-title":"Characterization of the human mineralocorticoid receptor gene 5\u2032-regulatory region: evidence for differential hormonal regulation of two alternative promoters via nonclassical mechanisms.","volume":"10","author":"Zennaro","year":"1996","journal-title":"Mol Endocrinol"}],"container-title":["Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/mend\/article-pdf\/17\/12\/2529\/10716605\/mend2529.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/mend\/article-pdf\/17\/12\/2529\/10716605\/mend2529.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T22:18:23Z","timestamp":1594937903000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mend\/article\/17\/12\/2529\/2747414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12,1]]},"references-count":60,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2003,12,1]]}},"URL":"https:\/\/doi.org\/10.1210\/me.2003-0299","relation":{},"ISSN":["0888-8809","1944-9917"],"issn-type":[{"value":"0888-8809","type":"print"},{"value":"1944-9917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12,1]]}}}