{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T08:23:29Z","timestamp":1721636609243},"reference-count":59,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The expression of caveolins is down-regulated in tissue samples of human thyroid autonomous adenomas and in the animal model of this disease. Because several cell types present in thyroid express caveolins, it remained unclear if this down-regulation occurs in thyrocytes and which are the mechanism and role of this down-regulation in the tumor context. Here we show that prolonged stimulation of isolated human thyrocytes by TSH\/cAMP\/cAMP-dependent protein kinase inhibits caveolins\u2019 expression. The expression of caveolins is not down-regulated by activators of other signaling pathways relevant to thyroid growth\/function. Therefore, the down-regulation of caveolins\u2019 expression in autonomous adenomas is a direct consequence of the chronic activation of the TSH\/cAMP pathway in thyrocytes. The down-regulation of caveolin-1 occurs at the mRNA level, with a consequent protein decrease. TSH\/cAMP induces a transcription-dependent, translation-independent destabilization of the caveolin-1 mRNA. This effect is correlated to the known proliferative role of that cascade in thyrocytes. In vivo, thyrocytes of caveolin-1 knockout mice display enhanced proliferation. This demonstrates, for the first time, the in vivo significance of the specific caveolin-1 down-regulation by one mitogenic cascade and its relation to a human disease.<\/jats:p>","DOI":"10.1210\/me.2006-0328","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T06:31:05Z","timestamp":1167892265000},"page":"921-932","source":"Crossref","is-referenced-by-count":10,"title":["Reciprocal Negative Regulation between Thyrotropin\/3\u2032,5\u2032-Cyclic Adenosine Monophosphate-Mediated Proliferation and Caveolin-1 Expression in Human and Murine Thyrocytes"],"prefix":"10.1210","volume":"21","author":[{"given":"Maria Jose\u0301","family":"Costa","sequence":"first","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (M.J.C., F.V.R., J.E.D., J.V.S.), Faculte\u0301 de Me\u0301decine, Universite\u0301 Libre de Bruxelles, 1070 Brussels, Belgium;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maximin","family":"Senou","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Me\u0301decine (M.S., C.D., M.-C.M.), Universite\u0301 Catholique de Louvain, 1200 Brussels, Belgium;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabienne","family":"Van Rode","sequence":"additional","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (M.J.C., F.V.R., J.E.D., J.V.S.), Faculte\u0301 de Me\u0301decine, Universite\u0301 Libre de Bruxelles, 1070 Brussels, Belgium;"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Ruf","sequence":"additional","affiliation":[{"name":"Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale Unite\u0301 555 (J.R.), Faculte\u0301 de Me\u0301decine Timone, Universite\u0301 de la Me\u0301diterrane\u0301e, 13284 Marseille, 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