{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:43:03Z","timestamp":1790775783102,"version":"4.1.0"},"reference-count":85,"publisher":"The Endocrine Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The p21-activated serine-threonine kinase (PAK1) is activated by small GTPase-dependent and -independent mechanisms and regulates cell motility. Both PAK1 and the hormone prolactin (PRL) have been implicated in breast cancer by numerous studies. We have previously shown that the PRL-activated tyrosine kinase JAK2 (Janus tyrosine kinase 2) phosphorylates PAK1 in vivo and identified tyrosines (Tyr) 153, 201, and 285 in the PAK1 molecule as sites of JAK2 tyrosyl phosphorylation. Here, we have used human breast cancer T47D cells stably overexpressing PAK1 wild type or PAK1 Y3F mutant in which Tyr(s) 153, 201, and 285 were mutated to phenylalanines to demonstrate that phosphorylation of these three tyrosines are required for maximal PRL-dependent ruffling. In addition, phosphorylation of these three tyrosines is required for increased migration of T47D cells in response to PRL as assessed by two independent motility assays. Finally, we show that PAK1 phosphorylates serine (Ser) 2152 of the actin-binding protein filamin A to a greater extent when PAK1 is tyrosyl phosphorylated by JAK2. Down-regulation of PAK1 or filamin A abolishes the effect of PRL on cell migration. Thus, our data presented here bring some insight into the mechanism of PRL-stimulated motility of breast cancer cells.<\/jats:p>","DOI":"10.1210\/me.2012-1291","type":"journal-article","created":{"date-parts":[[2013,1,23]],"date-time":"2013-01-23T07:27:13Z","timestamp":1358926033000},"page":"455-465","source":"Crossref","is-referenced-by-count":34,"title":["Tyrosyl Phosphorylated PAK1 Regulates Breast Cancer Cell Motility in Response to Prolactin through Filamin A"],"prefix":"10.1210","volume":"27","author":[{"given":"Alan","family":"Hammer","sequence":"first","affiliation":[{"name":"Department of Biological Sciences (A.H., L.R., P.O., L.C., M.D.), University of Toledo, Toledo, Ohio 43606-3390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah","family":"Rider","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (A.H., L.R., P.O., L.C., M.D.), University of Toledo, Toledo, Ohio 43606-3390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Oladimeji","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (A.H., L.R., P.O., L.C., M.D.), University of Toledo, Toledo, Ohio 43606-3390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie","family":"Cook","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences (A.H., L.R., P.O., L.C., M.D.), University of Toledo, Toledo, Ohio 43606-3390;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanwen","family":"Li","sequence":"additional","affiliation":[{"name":"the Department of Pharmacology (Q.L., R.R.M.), Wayne State University, Detroit, Michigan 48201"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond R.","family":"Mattingly","sequence":"additional","affiliation":[{"name":"the Department of Pharmacology (Q.L., R.R.M.), Wayne State University, Detroit, Michigan 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Res"},{"key":"2020071613353341300_B6","first-page":"1139","article-title":"Involvement of SHC, GRB2, SOS and RAS in prolactin signal transduction in mammary epithelial cells","volume":"13","author":"Das","year":"1996","journal-title":"Oncogene"},{"key":"2020071613353341300_B7","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/BF01806209","article-title":"Activation of raf-1, MEK, and MAP kinase in prolactin responsive mammary cells","volume":"40","author":"Das","year":"1996","journal-title":"Breast Cancer Res Treat"},{"key":"2020071613353341300_B8","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0303-7207(92)90102-C","article-title":"Prolactin-induced protein kinase C activity in a mouse mammary cell line (NOG-8)","volume":"90","author":"Banerjee","year":"1992","journal-title":"Mol Cell Endocrinol"},{"key":"2020071613353341300_B9","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/0303-7207(89)90092-0","article-title":"Role of protein kinase C in the prolactin-induced responses in mouse mammary gland explants","volume":"63","author":"Waters","year":"1989","journal-title":"Mol Cell Endocrinol"},{"key":"2020071613353341300_B10","first-page":"1461","article-title":"Prolactin receptor is associated with c-src kinase in rat liver","volume":"9","author":"Berlanga","year":"1995","journal-title":"Mol Endocrinol"},{"key":"2020071613353341300_B11","first-page":"674","article-title":"The protein tyrosine kinase P59fyn is associated with prolactin (PRL) receptor and is activated by PRL stimulation of T-lymphocytes","volume":"8","author":"Clevenger","year":"1994","journal-title":"Mol Endocrinol"},{"key":"2020071613353341300_B12","doi-asserted-by":"crossref","first-page":"2050","DOI":"10.1074\/jbc.272.4.2050","article-title":"Prolactin activates tyrosyl phosphorylation of insulin receptor substrate 1 and phosphatidylinositol-3-OH kinase","volume":"272","author":"Berlanga","year":"1997","journal-title":"J Biol 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Chem"},{"key":"2020071613353341300_B51","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1210\/endo.135.4.7925093","article-title":"JAK2 activation and cell proliferation induced by antibody-mediated prolactin receptor dimerization","volume":"135","author":"Rui","year":"1994","journal-title":"Endocrinology"},{"key":"2020071613353341300_B52","doi-asserted-by":"crossref","first-page":"10590","DOI":"10.1074\/jbc.274.15.10590","article-title":"SH2-B is required for nerve growth factor-induced neuronal differentiation","volume":"274","author":"Rui","year":"1999","journal-title":"J Biol Chem"},{"key":"2020071613353341300_B53","doi-asserted-by":"crossref","first-page":"6985","DOI":"10.1128\/MCB.16.12.6985","article-title":"Direct association with and dephosphorylation of Jak2 kinase by the SH2-domain-containing protein tyrosine phosphatase SHP-1","volume":"16","author":"Jiao","year":"1996","journal-title":"Mol Cell 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