{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T11:03:55Z","timestamp":1779275035964,"version":"3.51.4"},"reference-count":77,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bone morphogenetic proteins induce chondrogenesis and osteogenesis in vivo. To investigate molecular mechanisms involved in chondrocyte induction, we examined the effect of osteogenic protein (OP)-1\/bone morphogenetic protein-7 on the collagen X promoter. In rat calvaria-derived chondrogenic C5.18 cells, OP-1 up-regulates collagen X mRNA levels and its promoter activity in a cell type- specific manner. Deletion analysis localizes the OP-1 response region to 33 bp (\u2212310\/\u2212278), which confers OP-1 responsiveness to both the minimal homologous and heterologous Rous sarcoma virus promoter. Transforming growth factor-\u03b22 or activin, which up-regulates the expression of a transforming growth factor-\u03b2-inducible p3TP-Lux construct, has little effect on collagen X mRNA and on this 33-bp region. Mutational analysis shows that both an AP-1 like sequence (\u2212294\/\u2212285, TGAATCATCA) and an A\/T-rich myocyte enhancer factor (MEF)-2 like sequence (\u2212310\/\u2212298, TTAAAAATAAAAA) in the 33-bp region are necessary for the OP-1 effect. Gel shift assays show interaction of distinct nuclear proteins from C5.18 cells with the AP-1-like and the MEF-2- like sequences. OP-1 rapidly induces nuclear protein interaction with the MEF-2-like sequence but not with the AP-1 like sequence. MEF-2-like binding activity induced by OP-1 is distinct from the MEF-2 family proteins present in C2C12 myoblasts, in which OP-1 does not induce collagen X mRNA or up-regulate its promoter activity. In conclusion, we identified a specific response region for OP-1 in the mouse collagen X promoter. Mutational and gel shift analyses suggest that OP-1 induces nuclear protein interaction with an A\/T-rich MEF-2 like sequence, distinct from the MEF-2 present in myoblasts, and up-regulates collagen X promoter activity, which also requires an AP-1 like sequence.<\/jats:p>","DOI":"10.1210\/mend.11.12.0022","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:29:34Z","timestamp":1389202174000},"page":"1832-1845","source":"Crossref","is-referenced-by-count":27,"title":["Osteogenic Protein-1 Up-Regulation of the Collagen X Promoter Activity Is Mediated by a MEF-2-Like Sequence and Requires an Adjacent AP-1 Sequence"],"prefix":"10.1210","volume":"11","author":[{"given":"Shun-ichi","family":"Harada","sequence":"first","affiliation":[{"name":"Department of Bone Biology and Osteoporosis Research (S.H., G.A.R.) Merck Research Laboratories, West Point, Pennsylvania 19486"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. Kuber","family":"Sampath","sequence":"additional","affiliation":[{"name":"Creative Biomolecules, Inc. (T.K.S.) Hopkinton, Massachusetts 01748"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane E.","family":"Aubin","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Cell Biology (J.E.A.) Faculty of Medicine University of Toronto Toronto, Ontario M5S 1A8, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gideon A.","family":"Rodan","sequence":"additional","affiliation":[{"name":"Department of Bone Biology and Osteoporosis Research (S.H., G.A.R.) 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