{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:27:37Z","timestamp":1791217657876,"version":"4.1.0"},"reference-count":66,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) are ligand-dependent transcription factors. Although it is generally accepted that GR is translocated into the nucleus from the cytoplasm only after ligand binding, the subcellular localization of MR is still quite controversial. We examined the intracellular trafficking of MR in living neurons and nonneural cells using a fusion protein of green fluorescent protein (GFP) and rat MR (GFP-MR). Corticosterone (CORT) induced a rapid nuclear accumulation of GFP-MR, whereas in the absence of ligand, GFP-MR was distributed in both cytoplasm and nucleus in the majority of transfected cells. Given the differential action of MR and GR in the central nervous system, it is important to elucidate how the trafficking of these receptors between cytoplasm and nucleus is regulated by ligand. To examine the simultaneous trafficking of MR and GR within single living cells, we use different spectral variants of GFP, yellow fluorescent protein (YFP) and cyan fluorescent protein (CFP), linked to MR and GR, respectively. In COS-1 cells, expressing no endogenous corticosteroid receptors, the YFP-MR chimera was accumulated in the nucleus faster than the CFP-GR chimera in the presence of 10\u22129m CORT, while there was no significant difference in the nuclear accumulation rates in the presence of 10\u22126m CORT. On the other hand, in primary cultured hippocampal neurons expressing endogenous receptors, the nuclear accumulation rates of the YFP-MR chimera and CFP-GR chimera were nearly the same in the presence of both concentrations of CORT. These results suggest that CORT-induced nuclear translocation of MR and GR exhibits differential patterns depending on ligand concentrations or cell types.<\/jats:p>","DOI":"10.1210\/mend.15.7.0659","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:30:54Z","timestamp":1389202254000},"page":"1077-1092","source":"Crossref","is-referenced-by-count":92,"title":["Dynamic Changes in Subcellular Localization of Mineralocorticoid Receptor in Living Cells: In Comparison with Glucocorticoid Receptor using Dual-Color Labeling with Green Fluorescent Protein Spectral Variants"],"prefix":"10.1210","volume":"15","author":[{"given":"Mayumi","family":"Nishi","sequence":"first","affiliation":[{"name":"Department of Anatomy and Neurobiology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji, Kamigyo-ku Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ogawa","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji, Kamigyo-ku Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takao","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji, Kamigyo-ku Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-Ichi","family":"Matsuda","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji, Kamigyo-ku Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuhiro","family":"Kawata","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology Kyoto Prefectural University of Medicine Kawaramachi Hirokoji, Kamigyo-ku Kyoto 602-8566, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071617470669700_R1","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1210\/endo-117-6-2505","article-title":"Two receptor systems for corticosterone in rat brain: microdistribution and differential occupation.","volume":"117","author":"Reul","year":"1985","journal-title":"Endocrinology"},{"key":"2020071617470669700_R2","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/0896-6273(89)90177-3","article-title":"A molecular frame work for the action of glucocorticoid hormones in the nervous system.","volume":"2","author":"Evans","year":"1989","journal-title":"Neuron"},{"key":"2020071617470669700_R3","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.ge.19.120185.001233","article-title":"Steroid receptor regulated transcription of genes and gene network.","volume":"19","author":"Yamamoto","year":"1985","journal-title":"Annu Rev Genet"},{"key":"2020071617470669700_R4","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1146\/annurev.bi.63.070194.002315","article-title":"Molecular mechanism of action of steroid\/thyroid receptor superfamily members.","volume":"63","author":"Tsai","year":"1994","journal-title":"Annu Rev Biochem"},{"key":"2020071617470669700_R5","first-page":"233","author":"Gas","year":"1987","journal-title":"Steroid Hormone Receptors: Their Intracellular Localization."},{"key":"2020071617470669700_R6","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1210\/endo-120-4-1232","article-title":"Intracellular localization of the glucocorticoid receptor: evidence for cytoplasmic and nuclear localization.","volume":"120","author":"Wikstrom","year":"1987","journal-title":"Endocrinology"},{"key":"2020071617470669700_R7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0960-0760(94)00155-F","article-title":"Subcellular distribution of the glucocorticoid receptor and evidence for its association with microtubules.","volume":"52","author":"Akner","year":"1995","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071617470669700_R8","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1210\/endo-125-1-192","article-title":"Immunolocalization of renal mineralocorticoid receptors with an antiserum against a peptide deduced from the complementary deoxyribonucleic acid sequence.","volume":"125","author":"Krozowski","year":"1989","journal-title":"Endocrinology"},{"key":"2020071617470669700_R9","first-page":"1226","article-title":"Demonstration of nuclear translocation of the mineralocorticoid receptor (MR) using anti-MR antibody and confocal laser scanning microscopy.","volume":"7","author":"Robertson","year":"1993","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R10","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1042\/bj3020191","article-title":"Differential intracellular localization of human mineralocorticoid receptor on binding of agonists and antagonists.","volume":"302","author":"Lombes","year":"1994","journal-title":"Biochem J"},{"key":"2020071617470669700_R11","doi-asserted-by":"crossref","first-page":"11899","DOI":"10.1073\/pnas.92.25.11899","article-title":"Localization, trafficking, and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells.","volume":"92","author":"Ogawa","year":"1995","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071617470669700_R12","doi-asserted-by":"crossref","first-page":"4845","DOI":"10.1073\/pnas.93.10.4845","article-title":"Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera.","volume":"93","author":"Htun","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071617470669700_R13","doi-asserted-by":"crossref","first-page":"1927","DOI":"10.1046\/j.1460-9568.1999.00616.x","article-title":"Real-time imaging of glucocorticoid receptor dynamics in living neurons and glial cells in comparison with non-neural cells.","volume":"11","author":"Nishi","year":"1999","journal-title":"Eur J Neurosci"},{"key":"2020071617470669700_R14","doi-asserted-by":"crossref","first-page":"C39","DOI":"10.1152\/ajpcell.1998.274.1.C39","article-title":"Aldosterone responsiveness of A6 cells is restored by cloned rat mineralocorticoid receptor","volume":"274","author":"Chen","year":"1998","journal-title":"Am J Physiol"},{"key":"2020071617470669700_R15","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1073\/pnas.95.6.2973","article-title":"Subcellualr localization of mineralocorticoid receptors in living cells: effects of receptor agonists and antagonists.","volume":"95","author":"Fejes-Toth","year":"1998","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071617470669700_R16","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1210\/endo-96-3-598","article-title":"Differences in corticosterone and dexamethasone binding to rat brain and pituitary.","volume":"96","author":"de Kloet","year":"1975","journal-title":"Endocrinology"},{"key":"2020071617470669700_R17","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1152\/physrev.1986.66.4.1121","article-title":"Adrenal steroid receptors and actions in the nervous system.","volume":"66","author":"McEwen","year":"1986","journal-title":"Physiol Rev"},{"key":"2020071617470669700_R18","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1146\/annurev.ph.49.030187.002145","article-title":"Adrenocorticosteroids and the brain.","volume":"49","author":"Funder","year":"1987","journal-title":"Annu Rev Physiol"},{"key":"2020071617470669700_R19","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0168-0102(96)01105-4","article-title":"Distribution of glucocorticoid receptor immunoreactivity and mRNA in the rat brain: an immunocytochemical and in situ hybridization study.","volume":"26","author":"Morimoto","year":"1996","journal-title":"Neurosci Res"},{"key":"2020071617470669700_R20","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/0896-6273(88)90136-5","article-title":"The neuronal mineralocorticoid receptor as a mediator of glucocorticoid response.","volume":"1","author":"Arriza","year":"1988","journal-title":"Neuron"},{"key":"2020071617470669700_R21","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1002\/jnr.490210113","article-title":"Distribution of the mineralocorticoid and the glucocorticoid receptor mRNAs in the rat hippocampus","volume":"21","author":"van Eekelen","year":"1988","journal-title":"J Neurosci Res"},{"key":"2020071617470669700_R22","first-page":"95","article-title":"Brain corticosteroid receptor balance and homeostatic control.","volume":"12","author":"de Kloet","year":"1991","journal-title":"Front Neuroendocrinol"},{"key":"2020071617470669700_R23","doi-asserted-by":"crossref","first-page":"6056","DOI":"10.1073\/pnas.80.19.6056","article-title":"Renal mineralocorticoid receptors and hippocampal corticosterone binding species have identical intrinsic steroid specificity.","volume":"80","author":"Krozowski","year":"1983","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071617470669700_R24","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1210\/endo-113-6-2043","article-title":"Characterization of rat brain aldosterone receptors reveals high affinity for corticosterone.","volume":"113","author":"Beaumont","year":"1988","journal-title":"Endocrinology"},{"key":"2020071617470669700_R25","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/0922-4106(93)90072-H","article-title":"Pharmacological and functional characterization of human mineralocorticoid and glucocorticoid receptor ligands.","volume":"247","author":"Rupprecht","year":"1993","journal-title":"Eur J Pharmacol"},{"key":"2020071617470669700_R26","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0168-0102(96)81278-8","article-title":"Roles of steroid hormones and their receptors in structural organization in the nervous system.","volume":"24","author":"Kawata","year":"1995","journal-title":"Neurosci Res"},{"key":"2020071617470669700_R27","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1210\/mend.12.6.0119","article-title":"Folding requirements of the ligand-binding domain of the human mineralocorticoid receptor.","volume":"12","author":"Couette","year":"1998","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R28","first-page":"3","article-title":"Assessment of glucocorticoid receptor-heat shock protein 90 interactions in vivo during nucleocytoplasmic trafficking.","volume":"10","author":"Yang","year":"1996","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R29","first-page":"705","article-title":"Stable and specific binding of heat shock protein 90 by geldanamycin disrupts glucocorticoid receptor function in intact cells.","volume":"10","author":"Whitesell","year":"1996","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R30","doi-asserted-by":"crossref","first-page":"4689","DOI":"10.1074\/jbc.273.8.4689","article-title":"Growth-related changes in phosphorylation of yeast RNA polymerase II.","volume":"273","author":"Patturajan","year":"1998","journal-title":"J Biol Chem"},{"key":"2020071617470669700_R31","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1242\/jcs.110.15.1781","article-title":"Nuclear distribution of transcription factors in relation to sites of transcription and RNA polymerase II.","volume":"110","author":"Grande","year":"1997","journal-title":"J Cell Sci"},{"key":"2020071617470669700_R32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0301-0082(94)90014-0","article-title":"Mineralocorticoid and glucocorticoid receptors in the brain. Implication for ion permeability and transmitter systems.","volume":"43","author":"Joels","year":"1994","journal-title":"Prog Neurobiol"},{"key":"2020071617470669700_R33","doi-asserted-by":"crossref","first-page":"S47","DOI":"10.1111\/j.1440-1681.1998.tb02300.x","article-title":"Aldosterone: fact, failure and the future","volume":"25","author":"Funder","year":"1998","journal-title":"Clin Exp Pharmacol Physiol Suppl"},{"key":"2020071617470669700_R34","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1023\/A:1022023207326","article-title":"Influence of gonadal steroids on brain corticosteroid receptors: a minireview.","volume":"22","author":"Turner","year":"1997","journal-title":"Neurochem Res"},{"key":"2020071617470669700_R35","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1210\/mend.13.1.0226","article-title":"Modulation of human mineralocorticoid receptor function by protein kinase A.","volume":"13","author":"Massaad","year":"1999","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R36","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/0960-0760(94)90087-6","article-title":"Microtubules are not required for glucocorticoid receptor mediated gene induction.","volume":"51","author":"Sazapary","year":"1994","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071617470669700_R37","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1002\/j.1460-2075.1987.tb02550.x","article-title":"Microtubules containing detyrosinated tubulin are less dynamic.","volume":"6","author":"Kreis","year":"1987","journal-title":"EMBO J"},{"key":"2020071617470669700_R38","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/S0962-8924(98)01420-2","article-title":"Dual-colour imaging with GFP variants.","volume":"9","author":"Ellenberg","year":"1999","journal-title":"Trends Cell Biol"},{"key":"2020071617470669700_R39","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1440-1681.1986.tb02393.x","article-title":"Nuclear localization of type I aldosterone binding sites in steroid-unexposed GH3 cell.","volume":"13","author":"Pearce","year":"1986","journal-title":"Clin Exp Pharmacol Physiol"},{"key":"2020071617470669700_R40","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0303-7207(97)04034-3","article-title":"Trafficking of the androgen receptor in living cells with fused green fluorescent protein-androgen receptor.","volume":"129","author":"Georget","year":"1997","journal-title":"Mol Cell Endocrinol"},{"key":"2020071617470669700_R41","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1210\/mend.14.8.0497","article-title":"Dynamics of intracellular movement and nucleocytoplasmic recycling of the ligand-activated androgen receptor in living cells.","volume":"14","author":"Tyagi","year":"2000","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R42","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0960-0760(95)00268-5","article-title":"Intracellular traffic of steroid hormone receptors.","volume":"56","author":"Guiochon-Mantel","year":"1996","journal-title":"J Steroid Biochem Mol Biol"},{"key":"2020071617470669700_R43","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0006-2952(94)90433-2","article-title":"Cytoplasmic-nuclear trafficking of progesterone receptor. In vivo study of the mechanism of action of antiprogestins.","volume":"47","author":"Guiochon-Mantel","year":"1994","journal-title":"Biochem Pharmacol"},{"key":"2020071617470669700_R44","doi-asserted-by":"crossref","first-page":"3003","DOI":"10.1242\/jcs.108.9.3003","article-title":"Localization of the glucocorticoid receptor in discrete clusters in the cell nucleus.","volume":"108","author":"van Steensel","year":"1995","journal-title":"J Cell Sci"},{"key":"2020071617470669700_R45","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1242\/jcs.109.4.787","article-title":"Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampal neurons.","volume":"109","author":"van Steensel","year":"1996","journal-title":"J Cell Sci"},{"key":"2020071617470669700_R46","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1038\/35007077","article-title":"High mobility of profiles in the mammalian cell nucleus.","volume":"404","author":"Phair","year":"2000","journal-title":"Nature"},{"key":"2020071617470669700_R47","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1126\/science.287.5456.1262","article-title":"The glucocorticoid receptor: rapid exchange with regulatory sites in the living cells.","volume":"287","author":"McNally","year":"2000","journal-title":"Science"},{"key":"2020071617470669700_R48","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1083\/jcb.150.1.41","article-title":"Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.","volume":"150","author":"Kruhlak","year":"2000","journal-title":"J Cell Biol"},{"key":"2020071617470669700_R49","doi-asserted-by":"crossref","first-page":"F1","DOI":"10.1083\/jcb.150.1.F1","article-title":"Seeking common ground in nuclear complexity","volume":"150","author":"Shopland","year":"2000","journal-title":"J Cell Biol"},{"key":"2020071617470669700_R50","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0960-9822(98)70039-9","article-title":"Import and export of the nuclear protein import receptor transportin by a mechanism independent of GTP hydrolysis.","volume":"8","author":"Nakielny","year":"1997","journal-title":"Curr Biol"},{"key":"2020071617470669700_R51","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1038\/386779a0","article-title":"Nucleocytoplasmic transport: signals, mechanisms and regulation.","volume":"386","author":"Nigg","year":"1997","journal-title":"Nature"},{"key":"2020071617470669700_R52","first-page":"390","article-title":"Immunocytochemical localization of glucocorticoid receptor in human gingival fibroblasts and evidence for a colocalization of glucocorticoid receptor with cytoplasmic microtubules.","volume":"53","author":"Akner","year":"1992","journal-title":"Eur J Cell Biol"},{"key":"2020071617470669700_R53","first-page":"119","article-title":"Glucocorticoid receptor structure and the initial events in signal transduction.","volume":"322","author":"Pratt","year":"1990","journal-title":"Prog Clin Biol Res"},{"key":"2020071617470669700_R54","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1083\/jcb.119.2.337","article-title":"The cytoskeleton and the cellular traffick of the progesterone receptor.","volume":"119","author":"Perrot-Applanat","year":"1992","journal-title":"J Cell Biol"},{"key":"2020071617470669700_R55","first-page":"1549","article-title":"Evidence that the FK506-binding immunophilin heat shock protein 56 is required for trafficking of the glucocorticoid receptor from the cytoplasm to the nucleus.","volume":"9","author":"Czar","year":"1995","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R56","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1210\/mend.12.12.0204","article-title":"Heat shock protein 90-dependent (geldanamycin-inhibited) movement of the glucocorticoid receptor through the cytoplasm to the nucleus requires intact cytoskeleton.","volume":"12","author":"Galigniana","year":"1998","journal-title":"Mol Endocrinol"},{"key":"2020071617470669700_R57","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/0006-8993(93)90524-Q","article-title":"Adrenal steroid receptor immunoreactivity in cells born in the adult rat dentate gyrus.","volume":"611","author":"Cameron","year":"1993","journal-title":"Brain Res"},{"key":"2020071617470669700_R58","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.1749-6632.1994.tb39204.x","article-title":"Brain mineralocorticoid receptor function.","volume":"746","author":"de Kloet","year":"1994","journal-title":"Ann NY Acad Sci"},{"key":"2020071617470669700_R59","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.1126\/science.288.5472.1796","article-title":"Kinesin superfamily motor protein KIF 17 and mLin-10 in NMDA receptor-containing vesicle transport.","volume":"288","author":"Setou","year":"2000","journal-title":"Science"},{"key":"2020071617470669700_R60","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/S0303-7207(97)00115-9","article-title":"Inhibition of mineralocorticoid and glucocorticoid receptor function by the heat shock protein 90-binding agent geldanamycin.","volume":"131","author":"Bamberger","year":"1997","journal-title":"Mol Cell Endocrinol"},{"key":"2020071617470669700_R61","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1046\/j.1471-4159.1999.0720095.x","article-title":"Geldanamycin provides posttreatment protection against glutamate-induced oxidative toxicity in a mouse hippocampal cell line.","volume":"72","author":"Xiao","year":"1999","journal-title":"J Neurochem"},{"key":"2020071617470669700_R62","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1080\/713803536","article-title":"Geldanamycin induces heat shock protein expression through activation of HSF1 in K562 erythroleukemic cells.","volume":"48","author":"Kim","year":"1999","journal-title":"IUBMB Life"},{"key":"2020071617470669700_R63","first-page":"359","article-title":"Steroid regulation of neurotrophic activity: primary microcultures of midbrain raphe and hippocampus. In: deKloet ER, Sutanto W (eds) Methods in Neuroscience: Neurobiology of Steroids.","volume":"22","author":"Azmitia","year":"1994","journal-title":"Academic Press, San Diego, CA, vol"},{"key":"2020071617470669700_R64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/(SICI)1098-2396(199605)23:1<1::AID-SYN1>3.0.CO;2-E","article-title":"Enhanced synaptophysin immunoreactivity in rat hippocampal culture by 5-HT1A agonist, S100b, and corticosteroid receptor agonists.","volume":"23","author":"Nishi","year":"1996","journal-title":"Synapse"},{"key":"2020071617470669700_R65","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0168-0102(00)00112-7","article-title":"In vitro and in vivo immunohistochemistry for the distribution of mineralocorticoid receptor with specific antibody.","volume":"37","author":"Ito","year":"2000","journal-title":"Neurosci Res"},{"key":"2020071617470669700_R66","first-page":"101","article-title":"Expression and regulation of Escherichia coli lacZ gene fusion in mammalian cells.","volume":"2","author":"Hall","year":"1983","journal-title":"J Mol Appl Genet"}],"container-title":["Molecular Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/mend\/article-pdf\/15\/7\/1077\/8949616\/mend1077.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/mend\/article-pdf\/15\/7\/1077\/8949616\/mend1077.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:28:39Z","timestamp":1647908919000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/mend\/article\/15\/7\/1077\/2747995"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7,1]]},"references-count":66,"aliases":["10.1210\/me.15.7.1077"],"journal-issue":{"issue":"7","published-print":{"date-parts":[[2001,7,1]]}},"URL":"https:\/\/doi.org\/10.1210\/mend.15.7.0659","relation":{},"ISSN":["0888-8809","1944-9917"],"issn-type":[{"value":"0888-8809","type":"print"},{"value":"1944-9917","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7,1]]}}}