{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T22:34:28Z","timestamp":1792276468181,"version":"4.4.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The efflux transport of oestrone-3-sulphate, a steroid hormone sulphate, across the blood-cerebrospinal fluid barrier has been examined following its intracerebroventricular administration. [3H]Oestrone-3-sulphate was eliminated from cerebrospinal fluid (CSF) with an apparent efflux clearance of 205 \u03bcL min\u22121 per rat. There was 25% of unmetabolized [3H]oestrone-3-sulphate in the plasma 5 min after intracerebroventricular administration, indicating that at least a part of [3H]oestrone-3-sulphate is transported from CSF to the circulating blood across the blood-CSF barrier. This efflux transport was inhibited by co-administration of excess oestrone-3-sulphate (25 mm 10 \u03bcL = 0.25 \u03bcmol) into rat cerebral ventricle.<\/jats:p>\n               <jats:p>To characterize the oestrone-3-sulphate transport process, an in-vitro uptake experiment was performed using isolated rat choroid plexus. Oestrone-3-sulphate uptake by isolated rat choroid plexus was found to be a saturable process with a Michaelis-Menten constant (Km) of 18.1 \u00b1 6.3 \u03bcm, and a maximum uptake rate (Vmax) of 48.0\u00b1 15.1 pmol min\u22121 \u03bcL\u22121 of tissue. The oestrone-3-sulphate transport process was temperature dependent and was inhibited by metabolic inhibitors such as 2,4-dinitrophenol and rotenone, suggesting an energy dependence. This uptake process was also inhibited by steroid hormone sulphates (1 mm dehydroepiandrosterone sulphate and 1 mm oestrone sulphate), bile acids (1 mm taurocholic acid and 1 mm cholic acid) and organic anions (1 mm sulphobromophthalein and 1 mm phenolsulphonphthalein), whereas 1 mm \u00a0p-aminohippuric acid, 1 mm \u00a0p-nitrophenol sulphate, 0.1 mm methotrexate and the cardiac glycoside, 2.5 \u03bcm digoxin, had little effect.<\/jats:p>\n               <jats:p>In conclusion, these results provide evidence that oestrone-3-sulphate is transported from CSF to the circulating blood across the blood-CSF barrier via a carrier-mediated efflux transport system.<\/jats:p>","DOI":"10.1211\/0022357001773968","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T22:04:05Z","timestamp":1033509845000},"page":"281-288","source":"Crossref","is-referenced-by-count":23,"title":["In-vivo and In-vitro Evidence of a Carrier-mediated Efflux Transport System for Oestrone-3-sulphate across the Blood-Cerebrospinal Fluid Barrier"],"prefix":"10.1093","volume":"52","author":[{"given":"Takeo","family":"Kitazawa","sequence":"first","affiliation":[{"name":"Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences ,","place":["Japan"]},{"name":"Graduate School of Pharmaceutical Sciences, The University of Tokyo , Hongo 7-3-1, Bunkyo-ku, Tokyo 113\u20130033,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-Ichi","family":"Hosoya","sequence":"additional","affiliation":[{"name":"Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences ,","place":["Japan"]},{"name":"CREST of Japan Science and Technology Corporation (JST) ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeo","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Graduate School of Pharmaceutical Sciences, The University of Tokyo , Hongo 7-3-1, Bunkyo-ku, Tokyo 113\u20130033,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Terasaki","sequence":"additional","affiliation":[{"name":"Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences ,","place":["Japan"]},{"name":"New Industry Creation Hatchery Center, Tohoku University , Aoba, Aramaki, Aoba-ku, Sendai 980\u20138578,","place":["Japan"]},{"name":"CREST of Japan Science and Technology Corporation (JST) ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2000,3,1]]},"reference":[{"key":"2024103110043449200_cit1","doi-asserted-by":"crossref","first-page":"22395","DOI":"10.1074\/jbc.273.35.22395","article-title":"Molecular characterization and tissue distribution of a new organic anion transporter subtype (oatp3) that transports thyroid hormones and taurocholate and comparison with oatp2","volume":"273","author":"Abe","year":"1998","journal-title":"J. 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