{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:55:07Z","timestamp":1790844907825,"version":"4.1.0"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study was conducted to investigate the protective effect of Asteracantha longifolia Linn (Acanthaceae) plant extract on carbon tetrachloride (CCl4)- and paracetamol-induced acute hepatotoxicity in mice. Hepatotoxicity was induced by the administration of a single intraperitoneal dose of CCl4 (0.5 mL kg\u22121 CCl4 in olive oil) in one model and in the other by administration of paracetamol1 (300 mg kg\u22121 in saline) orally, after a 16-h fast. An aqueous extract of the whole plant (0.9 g kg\u22121) was used on a pre- and post-treatment basis. Asteracantha reduced the alanine aminotransferase (ALT) level by 69.32% (P &amp;lt; 0.001) and increased the liver reduced glutathione level by 64.65% (P &amp;lt; 0.001) in the pre-treated group, 4 days after the administration of CCl4. A similar pattern was observed in the pre-treated group 4 h after the administration of paracetamol with a reduction in serum levels of ALT, aspartate aminotransferase and alkaline phosphatase enzymes by 65.04, 55.79 and 45.75% respectively (P &amp;lt; 0.001). Plant extract also increased the glutathione concentration of the liver significantly (P &amp;lt; 0.001). Histopathological studies also provided supportive evidence for results from the biochemical analysis with marked improvement in liver architecture being observed in the Asteracantha-treated groups. Pre-treatment showed better results than post-treatment in both hepatotoxic models. Overall results indicate that the aqueous extract of Asteracantha longifolia possesses hepatoprotective effects on CCl4- and paracetamol-induced hepatotoxicity in mice.<\/jats:p>","DOI":"10.1211\/0022357021792","type":"journal-article","created":{"date-parts":[[2003,10,14]],"date-time":"2003-10-14T20:27:53Z","timestamp":1066163273000},"page":"1413-1418","source":"Crossref","is-referenced-by-count":47,"title":["Protective effect of <i>Asteracantha longifolia<\/i> extract in mouse liver injury induced by carbon tetrachloride and paracetamol"],"prefix":"10.1093","volume":"55","author":[{"given":"R P","family":"Hewawasam","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, University of Ruhuna ,","place":["Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K A P W","family":"Jayatilaka","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, University of Ruhuna ,","place":["Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Pathirana","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, University of Ruhuna ,","place":["Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L K B","family":"Mudduwa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine, University of Ruhuna ,","place":["Sri Lanka"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2003,10,1]]},"reference":[{"key":"2024103109392929900_cit1","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1002\/ptr.818","article-title":"Effect of the traditional medicinal plants Rhazya stricta, Balanitis aegyptiaca and Haplophylum tuberculatum on paracetamol induced hepatotoxicity in mice","volume":"15","author":"Ali","year":"2001","journal-title":"Phytotherapy Res."},{"key":"2024103109392929900_cit2","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1146\/annurev.me.35.020184.003045","article-title":"Acetaminophen toxicity","volume":"35","author":"Black","year":"1984","journal-title":"Annu. Rev. Med."},{"key":"2024103109392929900_cit3","first-page":"125","article-title":"Lipid peroxidation and its role in toxicology","volume":"1","author":"Bus","year":"1979","journal-title":"Rev. Biochem. Toxicol."},{"key":"2024103109392929900_cit4","first-page":"17","article-title":"Acute toxicity and gross effects","volume-title":"Laboratory manual for pharmacological evaluation of natural products.","author":"Dhawan","year":"1998"},{"key":"2024103109392929900_cit5","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0009-2797(88)90075-0","article-title":"Inhibition of lipid peroxidation by disulfiram and diethyl dithio carbamate doesn't prevent hepatotoxin induced cell death in isolated rat hepatocytes: A study with allyl alcohol tertiary-butyl hydroperoxide, diethyl maleate, bromoisovalerylurea and carbon tetrachloride","volume":"66","author":"Dogterom","year":"1988","journal-title":"Chem. Biol. Interact."},{"key":"2024103109392929900_cit6","first-page":"215","article-title":"The effectiveness of antioxidants in reducing paracetamol induced damage subsequent to paracetamol activation","volume":"49","author":"Harman","year":"1985","journal-title":"Res. Commun. Chem. Pathol. Pharmacol."},{"key":"2024103109392929900_cit7","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1055\/s-2006-961906","article-title":"An evaluation of the potency of Osbeckia octandra and Melothria maderaspatana as antihepatotoxic agents","volume":"55","author":"Jayatilaka","year":"1989","journal-title":"Planta Med."},{"key":"2024103109392929900_cit8","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0378-8741(90)90020-T","article-title":"Effect of Melothria maderaspatana on carbon tetrachloride induced changes in rat hepatic microsomal drug metabolizing enzyme activity","volume":"30","author":"Jayatilaka","year":"1990","journal-title":"J. Ethnopharmacol."},{"key":"2024103109392929900_cit9","first-page":"8","volume-title":"Medicinal plants used in ceylon.","author":"Jayaweera","year":"1981"},{"key":"2024103109392929900_cit10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1159\/000136485","article-title":"Bromobenzene induced liver necrosis: Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite","volume":"11","author":"Jollow","year":"1974","journal-title":"Pharmacology"},{"key":"2024103109392929900_cit11","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1055\/s-2006-958032","article-title":"Effect of lignan enriched Fructus schisandrae extract on hepatic glutathione status in rats: Protection against carbon tetrachloride toxicity","volume":"61","author":"Ko","year":"1995","journal-title":"Planta Med."},{"key":"2024103109392929900_cit12","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/S0367-326X(00)00269-0","article-title":"Constituents of Asteracantha longifolia","volume":"72","author":"Misra","year":"2001","journal-title":"Fitoterapia"},{"key":"2024103109392929900_cit13","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1055\/s-2008-1040482","article-title":"Molecular mechanisms of the hepatotoxicity caused by acetaminophen","volume":"10","author":"Nelson","year":"1990","journal-title":"Semin. Liver Dis."},{"key":"2024103109392929900_cit14","doi-asserted-by":"crossref","first-page":"2617","DOI":"10.1016\/0024-3205(78)90378-8","article-title":"Reaction of the CCl4 related peroxy free radical (CCl3\u2013O\u2013O') with amino acids. Pulse radiolysis evidence","volume":"23","author":"Packer","year":"1978","journal-title":"Life Sci."},{"key":"2024103109392929900_cit15","first-page":"356","article-title":"Hepatic pharmacology: Mechanisms of action and classification of antinarcotic hepatoprotective agents","volume":"2","author":"Perrissoud","year":"1982","journal-title":"Trends Pharmacol. Sci."},{"key":"2024103109392929900_cit16","first-page":"103","article-title":"Quantitative evaluation of indices of hepatotoxicity","volume-title":"Toxicology of the liver","author":"Plaa","year":"1982"},{"key":"2024103109392929900_cit17","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/0163-7258(89)90050-8","article-title":"Mechanisms of carbon tetrachloride toxicity","volume":"43","author":"Recknagel","year":"1989","journal-title":"Pharmacol. Ther."},{"key":"2024103109392929900_cit18","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1136\/jcp.10.4.394","article-title":"A colorimetric method for the determination of serum levels of glutamic oxaloacetic acid and pyruvic acid transaminases","volume":"10","author":"Reitman","year":"1957","journal-title":"Am. J. Clin. Pathol."},{"key":"2024103109392929900_cit19","first-page":"1","article-title":"Biochemical studies of the liver","volume-title":"Biochemical mechanisms of liver injury.","author":"Slater","year":"1965"},{"key":"2024103109392929900_cit20","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1016\/S0021-9258(19)39733-9","article-title":"Acetaminophen induced oxidation of protein thiols: Contribution of impaired thiol-metabolizing enzymes to the breakdown of adenine nucleotides","volume":"265","author":"Tirmenstein","year":"1990","journal-title":"J. Biol. Chem."},{"key":"2024103109392929900_cit21","doi-asserted-by":"crossref","first-page":"2065","DOI":"10.1016\/0006-2952(87)90132-8","article-title":"The mechanism of paracetamol induced hepatotoxicty by 3,5-dialkyl substitution. The role of glutathione depletion and oxidative stress","volume":"36","author":"van de Straat","year":"1987","journal-title":"Biochem. Pharmacol."},{"key":"2024103109392929900_cit22","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1055\/s-2000-11202","article-title":"Improvement of phase 1 drug metabolism with Schisandra chinensis against CCl4 hepatotoxicity in a rat model","volume":"66","author":"Zhu","year":"2000","journal-title":"Planta Med."}],"container-title":["Journal of Pharmacy and Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1211%2F0022357021792","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/55\/10\/1413\/60259643\/0022357021792.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jpp\/article-pdf\/55\/10\/1413\/60259643\/0022357021792.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,31]],"date-time":"2024-10-31T10:10:31Z","timestamp":1730369431000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jpp\/article\/55\/10\/1413\/6148122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10,1]]},"references-count":22,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2003,10,1]]},"published-print":{"date-parts":[[2003,10,1]]}},"URL":"https:\/\/doi.org\/10.1211\/0022357021792","relation":{},"ISSN":["0022-3573","2042-7158"],"issn-type":[{"value":"0022-3573","type":"print"},{"value":"2042-7158","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2003,10]]},"published":{"date-parts":[[2003,10,1]]}}}