{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:12:04Z","timestamp":1762099924747,"version":"3.28.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We examined the effects of ruscogenin glycoside (Lm-3), isolated from Liriope muscari, on lymphocyte adhesion to extracellular matrix. Adhesion of Jurkat cells activated by anti-CD3 to type I collagen was inhibited by Lm-3 in a concentration- and time-dependent manner. Lm-3 also inhibited the cell attachment to fibronectin and laminin. However, the saponin did not influence anti-CD3-induced cell proliferation and Mn2+-induced adhesion. Protein kinase C activator, phorbol 12, 13-dibutyrate, significantly enhanced, while its inhibitor, chlorpromazine, almost completely blocked, the adhesion of anti-CD3-activated Jurkat cells to collagen. Against phorbol 12,13-dibutyrate-activated Jurkat cells, Lm-3 treatment, either before or after activation, significantly inhibited the cell adhesion to collagen. Lm-3 also inhibited the adhesion activated by both anti-CD3 and phorbol 12,13-dibutyrate. Similar inhibition by Lm-3 of the phorbol 12,13-dibutyrate-induced adhesion to collagen was also observed in lymphocytes freshly isolated from mice with contact dermatitis. Furthermore, Lm-3 significantly decreased the leucocyte accumulation in an animal model of experimental pleurisy. These results suggest that the blockade of lymphocyte adhesion to extracellular matrix through interference with the protein kinase C pathway may be one of the mechanisms by which Lm-3 exerts anti-inflammatory activity.<\/jats:p>","DOI":"10.1211\/002235702760089081","type":"journal-article","created":{"date-parts":[[2002,10,11]],"date-time":"2002-10-11T20:14:04Z","timestamp":1034367244000},"page":"959-965","source":"Crossref","is-referenced-by-count":22,"title":["Ruscogenin glycoside (Lm-3) isolated from <i>Liriope muscari<\/i> inhibits lymphocyte adhesion to extracellular matrix"],"prefix":"10.1093","volume":"54","author":[{"given":"Jianli","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University , Nanjing 210038,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicinal Prescription, China Pharmaceutical University , Nanjing 210038,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boyang","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Chinese Medicinal Prescription, China Pharmaceutical University , Nanjing 210038,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology for Chinese Materia Medica, China Pharmaceutical University , Nanjing 210038,","place":["China"]},{"name":"State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University , Nanjing 210093,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,7,1]]},"reference":[{"key":"2024103109385040100_cit1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.3109\/08916939808993834","article-title":"T cell interactions with extracellular matrix proteins in patients with thyroid-associated ophthalmopathy","volume":"27","author":"Bednarczuk","year":"1998","journal-title":"Autoimmunity"},{"key":"2024103109385040100_cit2","first-page":"391","article-title":"The use of synthetic analogues of Arg-Gly-Asp (RGD) and soluble receptor of tumor necrosis factor to prevent acute and chronic experimental liver injury","volume":"70","author":"Bruck","year":"1997","journal-title":"Yale J. 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