{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:19:00Z","timestamp":1773299940109,"version":"3.50.1"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The influence of the plant product magnolol on neutrophil superoxide anion (O2-.) generation has been investigated in the rat.<\/jats:p>\n               <jats:p>Intraperitoneal injection of magnolol (30mgkg-1) significantly inhibited the formyl-methionyl-leucyl-phenylalanine (fMLP)-induced respiratory burst in rat whole blood ex-vivo. Magnolol also inhibited the O2-. generation with an IC50 (concentration resulting in 50% inhibition) of 15.4 \u00b1 1.6 \u03bcM and O2 consumption in rat neutrophils in-vitro. Magnolol weakly inhibited the O2-. generation in the xanthine-xanthine oxidase system, decreased cellular cyclic AMP level and had no effect on cyclic GMP levels. It weakly inhibited neutrophil cytosolic protein kinase C activity but did not alter porcine heart protein kinase A activity. Magnolol attenuated fMLP-induced protein tyrosine phosphorylation with an IC50 of 24.0 \u00b1 1.9 \u03bcM and the phosphorylation of mitogen-activated protein kinase p42\/44 with an IC50 of 28.5 \u00b1 4.5 \u03bcM. However, magnolol alone activated neutrophil phospholipase D activity as determined by the formation of phosphatidic acid and phosphatidylethanol in the presence of ethanol. In the presence of NADPH, the arachidonate-activated NADPH oxidase activity in a cell-free system was weakly suppressed by magnolol.<\/jats:p>\n               <jats:p>These results suggest that the inhibition of respiratory burst in fMLP-activated neutrophils by magnolol is probably attributable mainly to the attenuation of protein tyrosine phosphorylation and p42\/44 mitogen-activated protein kinase activation, and partly to the suppression of protein kinase C and NADPH oxidase activities.<\/jats:p>","DOI":"10.1211\/0022357991772466","type":"journal-article","created":{"date-parts":[[2003,7,18]],"date-time":"2003-07-18T23:43:52Z","timestamp":1058571832000},"page":"285-294","source":"Crossref","is-referenced-by-count":22,"title":["Inhibition by Magnolol of Formylmethionyl-leucyl-phenyl alanine-induced Respiratory Burst in Rat Neutrophils"],"prefix":"10.1093","volume":"51","author":[{"given":"Jih P","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, China Medical College , Taichung,","place":["Republic of China"]},{"name":"Graduate Institute of Pharmaceutical Chemistry, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ei F","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shue L","family":"Raung","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling C","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lo T","family":"Tsao","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei L","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Taichung Veterans General Hospital, China Medical College , Taichung,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien C","family":"Chen","sequence":"additional","affiliation":[{"name":"National Research Institute of Chinese Medicine , Taipei, Taiwan,","place":["Republic of China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1999,3,1]]},"reference":[{"key":"2024103110040727500_cit1","doi-asserted-by":"crossref","first-page":"5592","DOI":"10.1016\/S0021-9258(18)45614-1","article-title":"Genistein, a specific inhibitor of tyrosine-specific protein kinase","volume":"262","author":"Akiyama","year":"1987","journal-title":"J. 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