{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:20:27Z","timestamp":1790918427016,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[1999,10,1]],"date-time":"1999-10-01T00:00:00Z","timestamp":938736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Long-circulating submicron lipid emulsions, stabilized with poly(ethylene glycol)-modified phosphatidylethanolamine (PEG-PE), are promising drug carriers with substantial capacity for solubilization of lipophilic anticancer agents. This study describes the conjugation of the anti-B-cell lymphoma monoclonal antibody LL2 to the surface of lipid-emulsion globules by use of a novel poly(ethylene glycol)-based heterobifunctional coupling agent.<\/jats:p>\n               <jats:p>The efficiency of coupling of LL2 to the lipid emulsion was 85% (approx.) and essentially independent of the LL2\/emulsion particle ratio and amount of surface-bound PEG-PE. Results from sucrose-gradient centrifugation and Sepharose CL-4B gel filtration indicated stable binding of the antibody to the emulsion. The immunoreactivity of the emulsion-LL2 conjugates was tested with alkaline phosphatase-conjugated LL2 against a monoclonal anti-idiotype antibody, WN. The binding of the conjugates to WN increased with increasing surface density of LL2 up to 40 monoclonal antibodies\/emulsion particle, and exceeded that for the free monoclonal antibody (approx. 20 molecules\/particle). Results from competitive-binding ELISA were indicative of similar displacement curves for free LL2 and emulsion-LL2 conjugates. Direct cellular ELISA revealed similar binding of emulsion-LL2 complexes to three types of Burkitt's lymphoma cell lines, Raji, Ramos and Daudi.<\/jats:p>\n               <jats:p>The results from this study indicate that emulsion-LL2 complexes might be a useful drug-carrier system for more specific delivery of anticancer drugs to B-cell malignancy.<\/jats:p>","DOI":"10.1211\/0022357991776787","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T22:04:05Z","timestamp":1033509845000},"page":"1099-1105","source":"Crossref","is-referenced-by-count":31,"title":["Conjugation of an Anti-B-cell Lymphoma Monoclonal Antibody, LL2, to Long-circulating Drug-carrier Lipid Emulsions"],"prefix":"10.1093","volume":"51","author":[{"given":"Bo B","family":"Lundberg","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Pharmacy, Abo Akademi University , BioCity P. O. Box 66, FIN-20521 Abo,","place":["Finland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Griffiths","sequence":"additional","affiliation":[{"name":"Immunomedics Inc , Morris Plains, NJ 07950,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans J","family":"Hansen","sequence":"additional","affiliation":[{"name":"Immunomedics Inc , Morris Plains, NJ 07950,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1999,10,1]]},"reference":[{"key":"2024103110050264200_cit1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0005-2736(91)90246-5","article-title":"Liposomes containing synthetic lipid derivatives of poly(ethylene glycol) show prolonged circulation half-lives in vivo","volume":"1066","author":"Allen","year":"1991","journal-title":"Biochim. Biophys. 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