{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:20:50Z","timestamp":1790871650194,"version":"4.1.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[1999,12,1]],"date-time":"1999-12-01T00:00:00Z","timestamp":944006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>For future investigation of possible perturbation of circadian rhythm in animal models of Parkinson's disease we needed an animal model providing lasting 80\u2013100% striatal dopaminergic depletion in rats, but without induced mortality. We have thus compared the effects of a single hydroxydopamine bilateral striatal lesion (SB-hydroxydopamine) with those of a double hydroxydopamine bilateral lesion (DB-hydroxydopamine) at the same dose (16 \u03bcg\/striatum) by HPLC determination of dopamine and 3,4-dihydrophenylacetic acid (dopac) levels in the striatum.<\/jats:p>\n               <jats:p>Two weeks after neurosurgery, SB-hydroxydopamine and DB-hydroxydopamine induced dopaminergic depletion of at least 81% compared with control groups. After eight weeks striatal dopaminergic depletion was only 60.97% in SB-hydroxydopamine rats, suggesting a compensatory phenomenon, whereas in DB-hydroxydopamine rats dopaminergic loss was stable at 88%. For the DB-hydroxydopamine group the dopac\/dopamine ratio was significantly increased at week 2 only, whereas no significant change was observed for other groups. This increase might be explained by increased dopamine turnover.<\/jats:p>\n               <jats:p>We have demonstrated that striatal DB-hydroxydopamine injection induces lasting 80\u2013100% neuronal loss, close to that observed in the disease in man, without induced mortality, and provides a tool which meets our experimental requirements.<\/jats:p>","DOI":"10.1211\/0022357991777038","type":"journal-article","created":{"date-parts":[[2003,7,18]],"date-time":"2003-07-18T23:43:52Z","timestamp":1058571832000},"page":"1405-1408","source":"Crossref","is-referenced-by-count":19,"title":["Time-dependent Striatal Dopamine Depletion after Injection of 6-Hydroxydopamine in the Rat. Comparison of Single Bilateral and Double Bilateral Lesions"],"prefix":"10.1093","volume":"51","author":[{"given":"Val\u00e9rie","family":"Ben","sequence":"first","affiliation":[{"name":"Department of Medical and Clinical Pharmacology (EA 2199), Faculty of Medicine , 27 Bd J. Moulin, F 13385 Marseille, Cedex 5,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Blin","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Pharmacology (EA 2199), Faculty of Medicine , 27 Bd J. Moulin, F 13385 Marseille, Cedex 5,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Bruguerolle","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Pharmacology (EA 2199), Faculty of Medicine , 27 Bd J. Moulin, F 13385 Marseille, Cedex 5,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[1999,12,1]]},"reference":[{"key":"2024103110045408100_cit1","first-page":"191","article-title":"Progressive supranuclear palsy: anatomoclinical and biochemical considerations","volume":"45","author":"Agid","year":"1987","journal-title":"Adv. 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