{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:40:59Z","timestamp":1768095659115,"version":"3.49.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Stress-induced premature senescence (SIPS) has been proposed as an in-vitro model for testing the long-term effects of stressful events and to find molecules\/natural extracts that protect against such stress. Premature senescence of human skin diploid fibroblasts (HDFs) can be induced by repeated subcytotoxic exposure to UVB, with the appearance of so-called biomarkers of senescence such as growth arrest, senescence-associated \u03b2-galactosidase activity, senescence-associated gene over-expression and the common 4977-bp mitochondrial DNA deletion. This model of UVB-induced premature senescence has been acknowledged as a robust in-vitro model in photoageing research. In this study, the potential anti-photoageing effects of a series of algal extracts were tested. The appearance of the biomarkers of UVB-induced premature senescence of HDFs was studied with or without algal extracts. One algal extract was shown to be particularly protective against UVB-induced SIPS. The results obtained here reinforce the notion that UVB-induced premature senescence of HDFs can be used to screen potential anti-photoageing compounds.<\/jats:p>","DOI":"10.1211\/jpp.58.12.0003","type":"journal-article","created":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T23:24:27Z","timestamp":1166657067000},"page":"1577-1583","source":"Crossref","is-referenced-by-count":17,"title":["Identification of potential anti-photoageing algal compounds using an in-vitro model of photoageing"],"prefix":"10.1093","volume":"58","author":[{"given":"F","family":"Debacq-Chainiaux","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP) ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Borlon","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP) ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"De Hertogh","sequence":"additional","affiliation":[{"name":"Research Unit in Molecular Biology (URBM), Department of Biology, University of Namur (FUNDP) ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Remacle","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP) ,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P-Y","family":"Morvan","sequence":"additional","affiliation":[{"name":"Codif International , St-Malo,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Vall\u00e9e","sequence":"additional","affiliation":[{"name":"Codif International , St-Malo,","place":["France"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Toussaint","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry and Cellular Biology, Department of Biology, University of Namur (FUNDP) ,","place":["Belgium"]},{"name":"StratiCELL Screening Technologies S.A. , Namur,","place":["Belgium"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,12,1]]},"reference":[{"key":"2024103109345395200_cit1","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1073\/pnas.85.14.5112","article-title":"Human skin fibroblasts in vitro differentiate along a terminal cell lineage","volume":"85","author":"Bayreuther","year":"1988","journal-title":"Proc. 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Med."},{"key":"2024103109345395200_cit10","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1379\/1466-1268(2002)007<0023:OOAJIH>2.0.CO;2","article-title":"Overexpression of apolipoprotein J in human fibroblasts protects against cytotoxicity and premature senescence induced by ethanol and tert-butylhydroperoxide","volume":"7","author":"Dumont","year":"2002","journal-title":"Cell Stress Chaperones"},{"key":"2024103109345395200_cit11","volume-title":"Heat stress response of Spirulina platensis as a function of salinity; effect on growth and photosynthetic parameters","author":"Dupr\u00e9","year":"2004"},{"key":"2024103109345395200_cit12","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1006\/bbrc.1997.6409","article-title":"Quantification of wild-type mitochondrial DNA and its 4,8-kb deletion in rat organs","volume":"233","author":"Filser","year":"1997","journal-title":"Biochem. Biophys. Res. 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