{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:40:10Z","timestamp":1771663210730,"version":"3.50.1"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In traditional oriental medicine, Yin-Chen-Hao decoction is used for the remedy of liver diseases such as hepatitis, fatty liver, hepatocirrhosis and jaundice. However, despite extensive pharmacological study, the molecular mechanism of the anti-inflammatory effect of Yin-Chen-Hao decoction is poorly understood. In this study, we have investigated the pharmacological action on the mechanism of concanavalin A-induced T cell-dependent hepatitis in mice. Concanavalin A administration resulted in a severe liver injury. This was shown through increased levels of serum transaminase and lactic dehydrogenase, and increased liver DNA fragmentation and caspase-3 activity. Pretreatment with the aqueous extract from Yin-Chen-Hao decoction dose-dependently inhibited the elevation in transaminase and lactic dehydrogenase activity, and reduced liver DNA fragmentation and caspase-3 levels. There was an improvement in histological changes including inflammatory infiltration, hepatocyte necrosis and degeneration, and Kupffer cell hyperplasia. In addition, Yin-Chen-Hao decoction significantly inhibited tumour necrosis factor-\u03b1 (TNF-\u03b1) production in-vitro and in-vivo. Moreover, the activation of nuclear factor kappa B (NF-kB), which regulates TNF-\u03b1 production, was blocked by Yin-Chen-Hao decoction in-vitro and in-vivo. In conclusion, Yin-Chen-Hao decoction was capable of regulating T-cell-mediated liver injury in-vivo. This event may have depended on the decrease of TNF-\u03b1 production through the inhibition of NF-kB activation.<\/jats:p>","DOI":"10.1211\/jpp.58.5.0013","type":"journal-article","created":{"date-parts":[[2006,4,23]],"date-time":"2006-04-23T23:14:28Z","timestamp":1145834068000},"page":"677-684","source":"Crossref","is-referenced-by-count":18,"title":["Aqueous extract of Yin-Chen-Hao decoction, a traditional Chinese prescription, exerts protective effects on concanavalin A-induced hepatitis in mice through inhibition of NF-<i>k<\/i>B"],"prefix":"10.1093","volume":"58","author":[{"given":"Hui","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing Military Command , 305 Zhongshandong Road, Nanjing 210002,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao-Hong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Integrated Traditional Chinese and Western Medicine, Nanjing General Hospital of Nanjing Military Command , 305 Zhongshandong Road, Nanjing 210002,","place":["China"]},{"name":"Nanjing Municipal Hospital of Traditional Chinese Medicine , 1 Jinling Road, Nanjing 210001,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Jun","family":"Xia","sequence":"additional","affiliation":[{"name":"First College of Medicine, Nanjing University of Traditional Chinese Medicine , 282 Hanzhong Road, Nanjing 210029,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao-Wen","family":"Jin","sequence":"additional","affiliation":[{"name":"First College of Medicine, Nanjing University of Traditional Chinese Medicine , 282 Hanzhong Road, Nanjing 210029,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"2024103109343725800_cit1","first-page":"41","article-title":"The protection of Yin-Chen-Hao decoction on the experimental liver injury","volume":"26","author":"Cao","year":"2002","journal-title":"J. Zhejiang Coll. Trad. Chinese Med."},{"key":"2024103109343725800_cit2","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1055\/s-2005-837790","article-title":"Chemical composition and antimicrobial activity of the essential oils of Artemisia scoparia and","volume":"71","author":"Cha","year":"2005","journal-title":"A. capillaries. Planta Med."},{"key":"2024103109343725800_cit3","first-page":"26","article-title":"Review of the study of Yin-Chen-Hao decoction in the past decade","volume":"5","author":"Chen","year":"1998","journal-title":"Infor. Trad. Chinese Med."},{"key":"2024103109343725800_cit4","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.jamcollsurg.2003.08.014","article-title":"Protection against concanavalin A-induced hepatocyte apoptosis by molsidomine is time-dependent","volume":"198","author":"Ding","year":"2004","journal-title":"J. Am. Coll. Surg."},{"key":"2024103109343725800_cit5","first-page":"190","article-title":"Concanavalin A-induced T-cell-mediated hepatic injury in mice: the role of tumor necrosis factor","volume":"21","author":"Gantner","year":"1995","journal-title":"Hepatology"},{"key":"2024103109343725800_cit6","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1053\/jhep.2002.30991","article-title":"Current and novel immunosuppressive therapy for autoimmune hepatitis","volume":"35","author":"Heneghan","year":"2002","journal-title":"Hepatology"},{"key":"2024103109343725800_cit7","first-page":"3","article-title":"Pharmacological study of Yin-Chen-Hao decoction on downregulation of blood lipids level","volume":"14","author":"Huang","year":"1992","journal-title":"Chinese Trad. Patent Med."},{"key":"2024103109343725800_cit8","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.autrev.2004.12.003","article-title":"T cell immunity in hepatitis B and hepatitis C virus infection: implications for auto-immunity","volume":"4","author":"Ichiki","year":"2005","journal-title":"Autoimmunity Rev"},{"key":"2024103109343725800_cit9","doi-asserted-by":"crossref","first-page":"1160","DOI":"10.1002\/hep.20438","article-title":"Leflunomide protects from T-cell-mediated liver injury in mice through inhibition of nuclear factor-kB","volume":"40","author":"Imose","year":"2004","journal-title":"Hepatology"},{"key":"2024103109343725800_cit10","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/BF02977716","article-title":"Scoparone from Artemisia capillaris inhibits the release of inflammatory mediators in RAW 264.7 cells upon stimulation of cells by interferon-gamma plus LPS","volume":"28","author":"Jang","year":"2005","journal-title":"Arch. Pharm. Res."},{"key":"2024103109343725800_cit11","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1053\/jhep.2002.36155","article-title":"In vivo regulation of inducible NO synthase in immunemediated liver injury in mice","volume":"36","author":"Koerber","year":"2002","journal-title":"Hepatology"},{"key":"2024103109343725800_cit12","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1084\/jem.179.5.1529","article-title":"T cell activation-associated hepatic injury: mediation by tumor necrosis and protection by interleukin 6","volume":"179","author":"Mizuhara","year":"1994","journal-title":"J. Exp. Med."},{"key":"2024103109343725800_cit13","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1084\/jem.191.8.1263","article-title":"Intrahepatic T cells in hepatitis B: viral control versus liver cell injury","volume":"191","author":"Rehermann","year":"2000","journal-title":"J. Exp. Med."},{"key":"2024103109343725800_cit14","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1006\/cyto.2002.1948","article-title":"Cytokine expression in three mouse models of experimental hepatitis","volume":"19","author":"Sass","year":"2002","journal-title":"Cytokine"},{"key":"2024103109343725800_cit15","doi-asserted-by":"crossref","first-page":"3690","DOI":"10.4049\/jimmunol.158.8.3690","article-title":"Calpain, an upstream regulator of thymocyte apoptosis","volume":"158","author":"Squier","year":"1997","journal-title":"J. Immunol"},{"key":"2024103109343725800_cit16","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1172\/JCI115836","article-title":"A T cell-dependent experimental liver injury in mice inducible by concanavalin A","volume":"90","author":"Tiegs","year":"1992","journal-title":"J. Clin. 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