{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T16:56:40Z","timestamp":1775235400755,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Glycyrrhizin, a biological active compound isolated from the liquorice root, has been used as a treatment for chronic hepatitis. We have examined the involvement of matrix metalloproteinase (MMP)-9 in the development of lipopolysaccharide (LPS) and D-galactosamine (GalN)-induced liver injury in mice. We also investigated the effect of glycyrrhizin on expression of MMP-9 in this model. Levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) increased after LPS\/GalN treatment. Expression of MMP-9 mRNA and protein was markedly up-regulated in liver tissues 6\u20138 h after LPS\/GalN treatment. Pretreatment with glycyrrhizin (50 mg kg\u22121) and the MMP inhibitor (5 mg kg\u22121) suppressed increases in serum levels of ALT and AST in mice treated with LPS\/GalN. Furthermore, glycyrrhizin inhibited levels of both mRNA and protein for MMP-9. Immunohistochemical reaction for MMP-9 was observed in macrophages\/monocytes infiltrated in the inflammatory area of liver injury. Glycyrrhizin reduced the infiltration of inflammatory cells and immunoreactive MMP-9 in liver injury. The results indicated that MMP-9 played a role in the development of LPS\/GalN-induced mouse liver injury, and suggested that an inhibition by glycyrrhizin of the acute liver injury may have been due to a down-regulation of MMP-9.<\/jats:p>","DOI":"10.1211\/jpp.60.1.0012","type":"journal-article","created":{"date-parts":[[2007,12,10]],"date-time":"2007-12-10T01:22:10Z","timestamp":1197249730000},"page":"91-97","source":"Crossref","is-referenced-by-count":41,"title":["Glycyrrhizin prevents of lipopolysaccharide\/D-galactosamine-induced liver injury through down-regulation of matrix metalloproteinase-9 in mice"],"prefix":"10.1093","volume":"60","author":[{"given":"Kazuki","family":"Abe","sequence":"first","affiliation":[{"name":"Pharmacological Research Department, Minophagen Pharmaceutical Co. , 2-2-3, Komatsubara, Zama-shi, Kanagawa 228-0002,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadayuki","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Department of Anatomy II, School of Dental Medicine, Tsurumi University , 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230\u20138501,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Wake","sequence":"additional","affiliation":[{"name":"Department of Anatomy II, School of Dental Medicine, Tsurumi University , 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230\u20138501,","place":["Japan"]},{"name":"Liver Research Unit, Minophagen pharmaceutical Co. , 8\u201310-22, Akasaka, Minato-ku, Tokyo 107-0052,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuji","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Anatomy II, School of Dental Medicine, Tsurumi University , 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230\u20138501,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshitsugu","family":"Sato","sequence":"additional","affiliation":[{"name":"Pharmacological Research Department, Minophagen Pharmaceutical Co. , 2-2-3, Komatsubara, Zama-shi, Kanagawa 228-0002,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideo","family":"Inoue","sequence":"additional","affiliation":[{"name":"Pharmacological Research Department, Minophagen Pharmaceutical Co. , 2-2-3, Komatsubara, Zama-shi, Kanagawa 228-0002,","place":["Japan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,1,1]]},"reference":[{"key":"2024103109361403600_cit1","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1002\/(SICI)1097-0142(19970415)79:8<1494::AID-CNCR8>3.0.CO;2-B","article-title":"The long term efficacy of glycyrrhizin in chronic hepatitis C patients","volume":"79","author":"Arase","year":"1997","journal-title":"Cancer"},{"key":"2024103109361403600_cit2","first-page":"G1195","article-title":"Neutrophil margination and extravasation in sinusoids and venules of liver during endotoxin-induced injury","volume":"272","author":"Chosay","year":"1997","journal-title":"Am. J. Physiol."},{"key":"2024103109361403600_cit3","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1016\/S0140-6736(03)13615-X","article-title":"Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus","volume":"361","author":"Cinatl","year":"2003","journal-title":"Lancet"},{"key":"2024103109361403600_cit4","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1038\/sj.bjp.0702747","article-title":"Enhancement by galactosamine of lipopolysaccharide (LPS)-induced tumour necrosis factor production and lethality: its suppression by LPS pretreatment","volume":"128","author":"Endo","year":"1999","journal-title":"Br. J. Pharmacol."},{"key":"2024103109361403600_cit5","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1007\/s10620-006-3177-0","article-title":"A long-term glycyrrhizin injection therapy reduces hepato-cellular carcinogenesis rate in patients with interferon-resistant active chronic hepatitis C: a cohort study of 1249 patients","volume":"51","author":"Ikeda","year":"2006","journal-title":"Dig. Dis. Sci."},{"key":"2024103109361403600_cit6","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1093\/toxsci\/kfh291","article-title":"Inhibition of matrix metalloproteinases minimizes hepatic microvascular injury in response to acetaminophen in mice","volume":"83","author":"Ito","year":"2005","journal-title":"Toxicol. 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