{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:28:45Z","timestamp":1774960125356,"version":"3.50.1"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T00:00:00Z","timestamp":1201824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The gastroprotective mechanism of the natural diterpene ferruginol was assessed in mice and rats. The involvement of gastric prostaglandins (PGE2), reduced glutathione, nitric oxide or capsaicin receptors was evaluated in mice either treated or untreated with indometacin, N-ethylmaleimide (NEM), N-nitro-L-arginine methyl ester (L-NAME) or ruthenium red, respectively, and then orally treated with ferruginol or vehicle. Gastric lesions were induced by oral administration of ethanol. The effects of ferruginol on the parameters of gastric secretion were assessed in pylorus-ligated rats. Gastric PGE2 content was determined in rats treated with ferruginol and\/or indometacin. The reduction of gastric glutathione (GSH) content was determined in rats treated with ethanol after oral administration of ferruginol, lansoprazole or vehicle. Finally, the acute oral toxicity was assessed in mice. Indometacin reversed the gastroprotective effect of ferruginol (25 mg kg\u22121) but not NEM, ruthenium red or L-NAME. The diterpene (25 mg kg\u22121) increased the gastric juice volume and its pH value, and reduced the titrable acidity but was devoid of effect on the gastric mucus content. Ferruginol (25, 50 mg kg\u22121) increased gastric PGE2 content in a dose-dependent manner and prevented the reduction in GSH observed due to ethanol-induced gastric lesions in rats. Single oral doses up to 3 g kg\u22121 ferruginol did not elicit mortality or acute toxic effects in mice. Our results showed that ferruginol acted as a gastroprotective agent stimulating the gastric PGE2 synthesis, reducing the gastric acid output and improving the antioxidant capacity of the gastric mucosa by maintaining the GSH levels.<\/jats:p>","DOI":"10.1211\/jpp.60.2.0014","type":"journal-article","created":{"date-parts":[[2008,1,22]],"date-time":"2008-01-22T00:48:07Z","timestamp":1200962887000},"page":"245-251","source":"Crossref","is-referenced-by-count":30,"title":["Gastroprotective activity of ferruginol in mice and rats: effects on gastric secretion, endogenous prostaglandins and non-protein sulfhydryls"],"prefix":"10.1093","volume":"60","author":[{"given":"Carlos","family":"Areche","sequence":"first","affiliation":[{"name":"Laboratorio de Productos Naturales, Instituto de Qu\u00edmica de Recursos Naturales, Universidad de Talca , Casilla 747, Talca,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Theoduloz","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias B\u00e1sicas Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad de Talca , Casilla 747, Talca,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tania","family":"Y\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias B\u00e1sicas Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad de Talca , Casilla 747, Talca,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alba R M","family":"Souza-Brito","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica, Instituto de Biologia, CP 6109, CEP 13083-970, UNICAMP , Campinas, SP,","place":["Brasil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor","family":"Barbastefano","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica, Instituto de Biologia, CP 6109, CEP 13083-970, UNICAMP , Campinas, SP,","place":["Brasil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora","family":"de Paula","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica, Instituto de Biologia, CP 6109, CEP 13083-970, UNICAMP , Campinas, SP,","place":["Brasil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson L","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Fisiologia e Biof\u00edsica, Instituto de Biologia, CP 6109, CEP 13083-970, UNICAMP , Campinas, SP,","place":["Brasil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo","family":"Schmeda-Hirschmann","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales, Instituto de Qu\u00edmica de Recursos Naturales, Universidad de Talca , Casilla 747, Talca,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime A","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Ciencias B\u00e1sicas Biom\u00e9dicas, Facultad de Ciencias de la Salud, Universidad de Talca , Casilla 747, Talca,","place":["Chile"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,2,1]]},"reference":[{"key":"2024103109355590900_cit1","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1046\/j.1365-2036.2001.01022.x","article-title":"Efficacy of ecabet sodium for Helicobacter pylori eradication triple therapy in comparison with a lansoprazole-based regimen","volume":"15","author":"Adachi","year":"2001","journal-title":"Aliment. Pharmacol. Ther."},{"key":"2024103109355590900_cit2","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/S1043-6618(03)00154-3","article-title":"Protective role of carnitine esters against alcohol-induced gastric lesions in rats","volume":"48","author":"Arafa","year":"2003","journal-title":"Pharmacol. Res."},{"key":"2024103109355590900_cit3","doi-asserted-by":"crossref","first-page":"4052","DOI":"10.3748\/wjg.v11.i26.4052","article-title":"Lansoprazole prevents experimental gastric injury induced by non-steroidal anti-inflammatory drugs through a reduction of mucosal oxidative damage","volume":"11","author":"Blandizzi","year":"2005","journal-title":"World J. Gastroenterol."},{"key":"2024103109355590900_cit4","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"7","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"2024103109355590900_cit5","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/S0031-9422(00)80328-3","article-title":"Phenolic diterpenoids of Podocarpus ferrugineus and other podocarps","volume":"23","author":"Cambie","year":"1984","journal-title":"Phytochemistry"},{"key":"2024103109355590900_cit6","first-page":"116P","article-title":"Proceedings: A method for the quantitative estimation of gastric barrier mucus","volume":"242","author":"Corne","year":"1974","journal-title":"J. Physiol."},{"key":"2024103109355590900_cit7","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1139\/y95-018","article-title":"Intraluminal pH modulates gastric prostaglandin synthesis","volume":"73","author":"Curtis","year":"1995","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"2024103109355590900_cit8","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1055\/s-1999-13995","article-title":"Antiulcerogenic mechanisms of dehydrocrotonin, a diterpene obtained from Croton cajucara","volume":"65","author":"Hiruma-Lima","year":"1999","journal-title":"Planta Med."},{"key":"2024103109355590900_cit9","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1248\/bpb.23.1465","article-title":"Evaluation of the gastroprotective activity of cordatin, a diterpene isolated from Aparisthmium cordatum (Euphorbiaceae)","volume":"23","author":"Hiruma-Lima","year":"2000","journal-title":"Biol. Pharm. 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Sci."},{"key":"2024103109355590900_cit16","first-page":"494","article-title":"Roles of prostaglandins, nitric oxide and the capsaicin-sensitive sensory nerves in gastro-protection produced by ecabet sodium","volume":"275","author":"Kinoshita","year":"1995","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"2024103109355590900_cit17","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0928-4257(01)00049-3","article-title":"Epidermal growth factor and prostaglandin E (2) accelerate mucosal recovery from stress-induced gastric lesions via inhibition of apoptosis","volume":"95","author":"Konturek","year":"2001","journal-title":"J. Physiol. 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