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We have investigated the effect of an aqueous extract of oregano (Origanum vulgare. L.) on lipid peroxidation and anti-oxidant status in 1,2-dimethylhydrazine (DMH)-induced rat colon carcinogenesis. We aimed to identify the important antioxidants present in Indian oregano using RP-HPLC. DMH (20 mgkg\u22121) was administered subcutaneously once a week for the first four weeks and then discontinued. Oregano was supplemented every day orally at a dose of 20, 40 or 60 mgkg\u22121 to different groups of rats for 15 weeks. After this time the rats were killed and the colons were examined visually and evaluated biochemically. The levels of lipid peroxidation products, such as thiobarbituric acid reactive substances and conjugated dienes were significantly higher in the liver whereas in caecum and colon the levels were lower in DMH-treated animals as compared with control rats. The levels of the anti-oxidants superoxide dismutase, catalase, reduced glutathione, glutathione reductase, glutathione peroxidase and glutathione-S-transferase were decreased in DMH-treated rats, but were significantly reversed on oregano supplementation. Oregano supplementation (40 mgkg\u22121) had a modulatory role on tissue lipid peroxidation and antioxidant profile in colon cancer-bearing rats, which suggested a possible anti-cancer property of oregano.<\/jats:p>","DOI":"10.1211\/jpp.60.6.0015","type":"journal-article","created":{"date-parts":[[2008,5,14]],"date-time":"2008-05-14T02:07:57Z","timestamp":1210730877000},"page":"787-794","source":"Crossref","is-referenced-by-count":27,"title":["Dose-dependent effect of oregano (<i>Origanum vulgare<\/i> L.) on lipid peroxidation and antioxidant status in 1,2-dimethylhydrazine-induced rat colon carcinogenesis"],"prefix":"10.1093","volume":"60","author":[{"given":"Thummala","family":"Srihari","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University , Annamalainagar-608002, Tamilnadu,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murugan","family":"Sengottuvelan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University , Annamalainagar-608002, Tamilnadu,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Namasivayam","family":"Nalini","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University , Annamalainagar-608002, Tamilnadu,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,6,1]]},"reference":[{"key":"2024103109360075300_cit1","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1046\/j.1472-765X.2003.01285.x","article-title":"Anti-bacterial activity of selected plant essential oils against Escherichia coli 0157:H7","volume":"36","author":"Burt","year":"2003","journal-title":"Lett. 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Ethnopharmacol."},{"key":"2024103109360075300_cit8","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1078\/094471103322331890","article-title":"Inhibition of endogenous glucose for hypoglycaemic effect of Spergularia purpurea in diabetic mice","volume":"10","author":"Eddouks","year":"2003","journal-title":"Phytomedicine"},{"key":"2024103109360075300_cit9","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/0003-9861(59)90090-6","article-title":"Tissue sulphydryl groups","volume":"82","author":"Ellman","year":"1959","journal-title":"Arch. Biochem. Biophys."},{"key":"2024103109360075300_cit10","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1016\/S0899-9007(02)00916-4","article-title":"Free radicals, antioxidants, and nutrition","volume":"18","author":"Fang","year":"2002","journal-title":"Nutrition"},{"key":"2024103109360075300_cit11","doi-asserted-by":"crossref","first-page":"2436","DOI":"10.1002\/1097-0142(197711)40:5+<2436::AID-CNCR2820400908>3.0.CO;2-U","article-title":"Investigations into the metabolism and mode of action of the colon carcinogens 1,2-dimethylhydrazine and azoxymethane","volume":"40","author":"Fiala","year":"1977","journal-title":"Cancer"},{"key":"2024103109360075300_cit12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/S0021-9673(01)00750-6","article-title":"Analysis of Origanum vulgare volatiles by direct thermal desorption coupled to gas chromatography-mass spectrometry","volume":"918","author":"Garcia","year":"2001","journal-title":"J. Chromatogr. A."},{"key":"2024103109360075300_cit13","doi-asserted-by":"crossref","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","article-title":"Glutathione S-transferases. The first enzymatic step in mercapturic acid formation","volume":"249","author":"Habig","year":"1974","journal-title":"J. Biol. Chem."},{"key":"2024103109360075300_cit14","doi-asserted-by":"crossref","first-page":"410","DOI":"10.4315\/0362-028X-66.3.410","article-title":"Effects of herbal essential oils used to extend the shelf life of freshwater-related Asian sea fish (Lates calcarifer)","volume":"66","author":"Harpaz","year":"2003","journal-title":"J. Food Protec."},{"key":"2024103109360075300_cit15","doi-asserted-by":"crossref","first-page":"445","DOI":"10.3109\/10409239509083491","article-title":"The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemo protection and drug resistance","volume":"30","author":"Hayes","year":"1995","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"2024103109360075300_cit16","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/s004320050282","article-title":"Superoxide dismutases in the human colorectal cancer sequence","volume":"125","author":"Janssen","year":"1999","journal-title":"J. Cancer Res. Clin. Oncol."},{"key":"2024103109360075300_cit17","first-page":"130","article-title":"A modified spectro-photometric assay of superoxide dismutase","volume":"21","author":"Kakkar","year":"1984","journal-title":"Indian J. Biochem. Biophys."},{"key":"2024103109360075300_cit18","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1211\/jpp.58.8.0014","article-title":"Dose-response efficacy of caraway (Carum carvi L.) on tissue lipid peroxidation and antioxidant profile in rat colon carcinogenesis","volume":"58","author":"Kamaleewari","year":"2006","journal-title":"J. Pharm. Pharmacol."},{"key":"2024103109360075300_cit19","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1023\/B:CYTO.0000039917.60348.45","article-title":"Effects of carvacrol on a human non-small cell lung cancer (NSCLC) cell line, A549","volume":"43","author":"Koparal","year":"2003","journal-title":"Cytotechnology"},{"key":"2024103109360075300_cit20","first-page":"13","volume-title":"Metabolism of xenobiotics and chemical carcinogenesis","author":"Lang","year":"1999"},{"key":"2024103109360075300_cit21","first-page":"65","article-title":"Protein measurement with the Folin phenol reagent","volume":"3","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"2024103109360075300_cit22","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S0955-2863(99)00035-2","article-title":"Influence of dietary fibre from coconut kernel (Cocus nucifera) on the 1,2-dimethylhydrazine-induced lipid peroxidation in rats","volume":"10","author":"Manoj","year":"1999","journal-title":"J. Nutr. Biochem."},{"key":"2024103109360075300_cit23","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/0891-5849(88)90089-5","article-title":"Lipid peroxidation in tumor cells","volume":"4","author":"Masotti","year":"1998","journal-title":"Free Radic. Biol. Med."},{"key":"2024103109360075300_cit24","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/0024-3205(74)90206-9","article-title":"Glutathione, metabolism and function via the gamma-glutamyl cycle","volume":"15","author":"Meister","year":"1974","journal-title":"Life Sci."},{"key":"2024103109360075300_cit25","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0031-9422(00)00474-X","article-title":"The essential oil of Origanum vulgare L. ssp. vulgare growing wild in Vilnius district (Lithuania)","volume":"57","author":"Mockute","year":"2001","journal-title":"Phytochemistry"},{"key":"2024103109360075300_cit26","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1111\/j.1349-7006.1999.tb00698.x","article-title":"Increased choline kinase activity in 1,2-dimethylhydrazine-induced rat colon cancer","volume":"90","author":"Nakagami","year":"1990","journal-title":"Jpn. J. Cancer Res."},{"key":"2024103109360075300_cit27","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","article-title":"Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction","volume":"95","author":"Ohkawa","year":"1979","journal-title":"Anal. Biochem."},{"key":"2024103109360075300_cit28","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0304-3835(02)00203-3","article-title":"Modulation of carcinogenic response and antioxidant enzymes of rats administered with 1,2-dimethylhydrazine by Picroliv","volume":"191","author":"Kumar","year":"2003","journal-title":"Cancer Lett."},{"key":"2024103109360075300_cit29","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0014-4800(68)90041-5","article-title":"Early onset of lipoperoxidation in rat liver after carbon tetrachloride administration","volume":"9","author":"Rao","year":"1968","journal-title":"Exp. Mol. 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