{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T05:22:54Z","timestamp":1740547374764,"version":"3.38.0"},"reference-count":10,"publisher":"IOP Publishing","issue":"T118","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/page\/copyright"},{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/iopscience.iop.org\/info\/page\/text-and-data-mining"}],"content-domain":{"domain":["iopscience.iop.org"],"crossmark-restriction":false},"short-container-title":["Phys. Scr."],"published-print":{"date-parts":[[2005,1,1]]},"abstract":"<jats:p>Cytochromes c<jats:sub>3<\/jats:sub> are soluble electron transfer proteins in the periplasm of sulphate-reducing bacteria. They act as electron-proton couplers between hydrogenase and the electron transfer chain of sulphate respiration. In this work, cytochrome c<jats:sub>3<\/jats:sub> (Cyt-c<jats:sub>3<\/jats:sub>) obtained from both <jats:italic>Desulfovibrio vulgaris<\/jats:italic> and <jats:italic>Desulfovibrio gigas<\/jats:italic>, is electrostatically adsorbed on Ag electrodes coated with self-assembled monolayers of 11-mercaptoundecanoic acid. The redox equilibria of the adsorbed tetraheme protein are studied by surface enhanced resonance Raman spectroscopy (SERRS).<\/jats:p>\n               <jats:p>The quantitative analysis of the SERR spectra, which were measured as a function of the electrode potential, allows determining the redox potentials for the individual hemes of Cyt-c<jats:sub>3<\/jats:sub>. The values obtained of the redox potentials are compared with the data provided by NMR experiments and by molecular dynamics simulation studies of the electrostatically bound protein on a coated electrode. It is found that immobilisation causes substantial shifts of the redox potential, which would have an impact on the intramolecular electron flow.<\/jats:p>","DOI":"10.1238\/physica.topical.118a00225","type":"journal-article","created":{"date-parts":[[2005,9,20]],"date-time":"2005-09-20T14:06:21Z","timestamp":1127225181000},"page":"225-227","update-policy":"https:\/\/doi.org\/10.1088\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Redox Equilibria of Cytochrome C<sub>3<\/sub> Immobilised on Self-Assembled Monolayers Coated Silver Electrodes"],"prefix":"10.1088","volume":"2005","author":[{"family":"R E Di Paolo","sequence":"first","affiliation":[]},{"family":"L Rivas","sequence":"additional","affiliation":[]},{"family":"D Murgida","sequence":"additional","affiliation":[]},{"family":"P Hildebrandt","sequence":"additional","affiliation":[]}],"member":"266","reference":[{"key":"1-1","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1002\/bip.10101","volume":"67","year":"2002","journal-title":"Biopolymers","ISSN":"https:\/\/id.crossref.org\/issn\/0006-3525","issn-type":"print"},{"key":"2-1","doi-asserted-by":"publisher","first-page":"15808","DOI":"10.1021\/bi981505t","volume":"37","year":"1998","journal-title":"Biochemistry","ISSN":"https:\/\/id.crossref.org\/issn\/0006-2960","issn-type":"print"},{"key":"3-1","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1111\/j.1432-1033.1996.00723.x","volume":"241","year":"1996","journal-title":"Eur. J. Biochem.","ISSN":"https:\/\/id.crossref.org\/issn\/0014-2956","issn-type":"print"},{"key":"4-1","doi-asserted-by":"publisher","first-page":"1578","DOI":"10.1021\/jp003742n","volume":"105","year":"2001","journal-title":"J. Phys. Chem.","ISSN":"https:\/\/id.crossref.org\/issn\/1089-5647","issn-type":"print"},{"key":"5-1","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1002\/(SICI)1520-6343(1998)4:3<161::AID-BSPY2>3.0.CO;2-7","volume":"4","year":"1998","journal-title":"Biospectrosc.","ISSN":"https:\/\/id.crossref.org\/issn\/1075-4261","issn-type":"print"},{"key":"6-1"},{"key":"7-1","doi-asserted-by":"publisher","first-page":"4823","DOI":"10.1021\/jp014175m","volume":"106","year":"2002","journal-title":"J. Phys. Chem.","ISSN":"https:\/\/id.crossref.org\/issn\/1089-5647","issn-type":"print"},{"key":"8-1","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1366\/0003702991946668","volume":"53","year":"1999","journal-title":"Appl. Spectrosc.","ISSN":"https:\/\/id.crossref.org\/issn\/0003-7028","issn-type":"print"},{"key":"9-1","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1002\/cphc.200200618","volume":"4","year":"2003","journal-title":"Chem. Phys. Chem.","ISSN":"https:\/\/id.crossref.org\/issn\/1439-4235","issn-type":"print"},{"key":"10-1"}],"container-title":["Physica Scripta"],"original-title":[],"link":[{"URL":"https:\/\/iopscience.iop.org\/article\/10.1238\/Physica.Topical.118a00225","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1238\/Physica.Topical.118a00225\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1238\/Physica.Topical.118a00225\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/stacks.iop.org\/1402-4896\/2005\/i=T118\/a=059\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/iopscience.iop.org\/article\/10.1238\/Physica.Topical.118a00225\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T11:22:39Z","timestamp":1740482559000},"score":1,"resource":{"primary":{"URL":"https:\/\/iopscience.iop.org\/article\/10.1238\/Physica.Topical.118a00225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,1]]},"references-count":10,"journal-issue":{"issue":"T118","published-print":{"date-parts":[[2005,1,1]]}},"URL":"https:\/\/doi.org\/10.1238\/physica.topical.118a00225","relation":{},"ISSN":["0031-8949","1402-4896"],"issn-type":[{"type":"print","value":"0031-8949"},{"type":"electronic","value":"1402-4896"}],"subject":[],"published":{"date-parts":[[2005,1,1]]},"assertion":[{"value":"Redox Equilibria of Cytochrome C3 Immobilised on Self-Assembled Monolayers Coated Silver Electrodes","name":"article_title","label":"Article Title"},{"value":"Physica Scripta","name":"journal_title","label":"Journal Title"},{"value":"paper","name":"article_type","label":"Article Type"},{"value":"Published under licence by IOP Publishing Ltd","name":"copyright_information","label":"Copyright Information"},{"value":"2003-10-18","name":"date_received","label":"Date Received","group":{"name":"publication_dates","label":"Publication dates"}},{"value":"2004-08-13","name":"date_accepted","label":"Date Accepted","group":{"name":"publication_dates","label":"Publication dates"}},{"name":"date_epub","label":"Online publication date","group":{"name":"publication_dates","label":"Publication dates"}}]}}