{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:18:09Z","timestamp":1740165489011,"version":"3.37.3"},"reference-count":34,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":1639,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["606788","APP1041929"],"award-info":[{"award-number":["606788","APP1041929"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003752","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP150100364","DE120102321"],"award-info":[{"award-number":["DP150100364","DE120102321"]}],"id":[{"id":"10.13039\/501100003752","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Chlamydia species are obligate intracellular pathogens that have a major impact on human health. The pathogen replicates within an intracellular niche called an inclusion and is thought to rely heavily on host-derived proteins and lipids, including ceramide. Sortilin is a transmembrane receptor implicated in the trafficking of acid sphingomyelinase, which is responsible for catalysing the breakdown of sphingomyelin to ceramide. In this study, we examined the role of sortilin in Chlamydia trachomatis L2 development. Western immunoblotting and immunocytochemistry analysis revealed that endogenous sortilin is not only associated with the inclusion, but that protein levels increase in infected cells. RNAi-mediated depletion of sortilin, however, had no detectable impact on ceramide delivery to the inclusion or the production of infectious progeny. This study demonstrates that whilst Chlamydia redirects sortilin trafficking to the chlamydial inclusion, RNAi knockdown of sortilin expression is insufficient to determine if this pathway is requisite for the development of the pathogen.<\/jats:p>","DOI":"10.1242\/bio.016485","type":"journal-article","created":{"date-parts":[[2016,3,10]],"date-time":"2016-03-10T03:59:30Z","timestamp":1457582370000},"page":"429-435","source":"Crossref","is-referenced-by-count":4,"title":["Sortilin is associated with the chlamydial inclusion and is modulated during infection"],"prefix":"10.1242","volume":"5","author":[{"given":"Wei Xuan","family":"Teo","sequence":"first","affiliation":[{"name":"Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus Charles","family":"Kerr","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilhelmina May","family":"Huston","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Technology Sydney, Broadway, New South Wales 2007, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohan David","family":"Teasdale","sequence":"additional","affiliation":[{"name":"Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2016,3,9]]},"reference":[{"key":"2021042423344464100_BIO016485C1","doi-asserted-by":"publisher","first-page":"e1004883","DOI":"10.1371\/journal.ppat.1004883","article-title":"The proteome of the isolated Chlamydia trachomatis containing vacuole reveals a complex trafficking platform enriched for retromer components","volume":"11","author":"Aeberhard","year":"2015","journal-title":"PLoS Pathog."},{"key":"2021042423344464100_BIO016485C2","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1242\/jcs.02733","article-title":"Trafficking from CD63-positive late endocytic multivesicular bodies is essential for intracellular development of Chlamydia trachomatis","volume":"119","author":"Beatty","year":"2006","journal-title":"J. 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