{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:48:36Z","timestamp":1787021316274,"version":"build-2736575974"},"reference-count":31,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":1084,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31260512"],"award-info":[{"award-number":["31260512"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30960054"],"award-info":[{"award-number":["30960054"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31040011"],"award-info":[{"award-number":["31040011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012579","name":"Natural Science Foundation of Qinghai Province","doi-asserted-by":"crossref","award":["2016-ZJ-901"],"award-info":[{"award-number":["2016-ZJ-901"]}],"id":[{"id":"10.13039\/501100012579","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012579","name":"Natural Science Foundation of Qinghai Province","doi-asserted-by":"crossref","award":["2014-ZJ-714"],"award-info":[{"award-number":["2014-ZJ-714"]}],"id":[{"id":"10.13039\/501100012579","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012579","name":"Natural Science Foundation of Qinghai Province","doi-asserted-by":"crossref","award":["2012-Z-905"],"award-info":[{"award-number":["2012-Z-905"]}],"id":[{"id":"10.13039\/501100012579","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The plateau pika (Ochotona curzoniae) is a species of sprint-running alpine animals in the Qinghai-Tibet Plateau, which is a harsh highland hypoxic environment. Ldh-c is expressed in the testis, sperm and somatic tissues of plateau pika. To reveal the role and physiological mechanisms of sperm-specific lactate dehydrogenase (LDH-C4), in plateau pika to adapt to hypoxic environment, an adenoviral line of pMultiRNAi-Ldhc was constructed and injected into the bilateral biceps femoris of the hind legs. The swimming times of the pikas, and the Ldh-c expression levels, total LDH activities and ATP levels in skeletal muscle, were measured after the pikas were raised in the trapped site for 5\u2005days. Our results showed that after Ldh-c was silenced, the sprint-running ability (swimming time) of the plateau pikas was significant decreased, and the total LDH activities and ATP levels were reduced by 28.21% and 27.88%, respectively. Our results indicated that expression of Ldh-c in the skeletal muscle of plateau pika increased anaerobic glycolysis and enhanced adaptation to highland hypoxic environments.<\/jats:p>","DOI":"10.1242\/bio.024943","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T04:30:12Z","timestamp":1505449812000},"page":"1336-1341","source":"Crossref","is-referenced-by-count":6,"title":["The expression of\n                    <i>Ldh-c<\/i>\n                    in the skeletal muscle of plateau pika (\n                    <i>Ochotona curzoniae<\/i>\n                    ) enhances adaptation to a hypoxic environment"],"prefix":"10.1242","volume":"6","author":[{"given":"Zhi F.","family":"An","sequence":"first","affiliation":[{"name":"State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University 10743, Xining, Qinghai 810016, China"},{"name":"Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0026-6362","authenticated-orcid":false,"given":"Deng B.","family":"Wei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University 10743, Xining, Qinghai 810016, China"},{"name":"Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai 810016, China"},{"name":"College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lian","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Research Center for High Altitude Medicine, Qinghai University, Xining, Qinghai 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin N.","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2017,9,15]]},"reference":[{"key":"2021042423511144900_BIO024943C1","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1126\/science.139.3555.601","article-title":"Lactate dehydrogenases in human testes","volume":"139","author":"Blanco","year":"1963","journal-title":"Science"},{"key":"2021042423511144900_BIO024943C2","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1042\/bj0970513","article-title":"Lactate dehydrogenase isoenzymes of human semen","volume":"97","author":"Clausen","year":"1965","journal-title":"Biochem. J."},{"key":"2021042423511144900_BIO024943C3","doi-asserted-by":"publisher","first-page":"502","DOI":"10.2164\/jandrol.05185","article-title":"Testis-Specific Lactate Dehydrogenase (LDH-C4; Ldh3) in murine oocytes and preimplantation embryos","volume":"27","author":"Coonrod","year":"2006","journal-title":"J. Androl."},{"key":"2021042423511144900_BIO024943C4","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1002\/jez.1402250305","article-title":"Catalytic properties of the sperm-specific lactate dehydrogenase (LDH X or C4) from different species","volume":"225","author":"Coronel","year":"1983","journal-title":"J. Exp. Zool."},{"key":"2021042423511144900_BIO024943C5","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1126\/science.139.3555.602","article-title":"Lactic and malic dehydrogenases in human spermatozoa","volume":"139","author":"Goldberg","year":"1963","journal-title":"Science"},{"key":"2021042423511144900_BIO024943C6","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1111\/j.1749-6632.1964.tb14226.x","article-title":"Lactate dehydrogenases and malate dehydrogenases in sperm: studied by polyacrylamide gel electrophoresis","volume":"121","author":"Goldberg","year":"1964","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2021042423511144900_BIO024943C7","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/S0076-6879(75)41072-2","article-title":"Lactate dehydrogenase-X from mouse testes and spermatozoa","volume":"41","author":"Goldberg","year":"1975","journal-title":"Methods Enzymol."},{"key":"2021042423511144900_BIO024943C8","first-page":"120","article-title":"Reproductive implications of LDH-C4 and other testis-specific isozymes","volume":"2","author":"Goldberg","year":"1984","journal-title":"Exp. Clin. Immunogenet."},{"key":"2021042423511144900_BIO024943C9","first-page":"431","article-title":"Comparative analysis in transport and intake of oxygen between pikas (Ochotona curzoniae) and rats","volume":"12","author":"He","year":"1994","journal-title":"J. Prevent Med. Chinese People's Liberation."},{"key":"2021042423511144900_BIO024943C10","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1093\/humrep\/der317","article-title":"Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa","volume":"26","author":"Hereng","year":"2011","journal-title":"Hum. Reprod."},{"key":"2021042423511144900_BIO024943C11","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/0003-2697(70)90066-7","article-title":"Determination of ATP in Chlorella with the luciferin-luciferase enzyme system","volume":"37","author":"John","year":"1970","journal-title":"Anal. Biochem."},{"key":"2021042423511144900_BIO024943C12","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1023\/A:1024161409110","article-title":"Keystone status of plateau pikas (Ochotona curzoniae): effect of control on biodiversity of native birds","volume":"12","author":"Lai","year":"2003","journal-title":"Biodivers. Conserv."},{"key":"2021042423511144900_BIO024943C13","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1042\/bj2730587","article-title":"Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli","volume":"273","author":"LeVan","year":"1991","journal-title":"Biochem. J."},{"key":"2021042423511144900_BIO024943C14","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1002\/jez.510","article-title":"The protein level of hypoxia-inducible factor-1\u03b1 is increased in the plateau pika (Ochotona curzoniae) inhabiting high altitudes","volume":"311","author":"Li","year":"2009","journal-title":"J. Exp. Zool A Ecol. Genet. Physiol."},{"key":"2021042423511144900_BIO024943C15","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1095\/biolreprod.103.026054","article-title":"Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement","volume":"71","author":"Mukai","year":"2004","journal-title":"Biol. Reprod."},{"key":"2021042423511144900_BIO024943C16","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1095\/biolreprod.108.068353","article-title":"Expression of the gene for mouse lactate dehydrogenase C (Ldhc) is required for male fertility","volume":"79","author":"Odet","year":"2008","journal-title":"Biol. Reprod."},{"key":"2021042423511144900_BIO024943C17","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1095\/biolreprod.111.091546","article-title":"Lactate dehydrogenase C and energy metabolism in mouse sperm","volume":"85","author":"Odet","year":"2011","journal-title":"Biol. Reprod."},{"key":"2021042423511144900_BIO024943C18","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1016\/j.bbrc.2003.11.177","article-title":"Guideline to reference gene selection for quantitative real-time PCR","volume":"313","author":"Radoni\u0107","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021042423511144900_BIO024943C19","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1111\/j.1469-1795.1999.tb00069.x","article-title":"The plateau pika (Ochotona curzoniae) is a keystone species for biodiversity on the Tibetan plateau","volume":"2","author":"Smith","year":"1999","journal-title":"Anim. Conserv."},{"key":"2021042423511144900_BIO024943C20","doi-asserted-by":"publisher","first-page":"358","DOI":"10.5483\/BMBRep.2008.41.5.358","article-title":"Multiple shrna expressing vector enhances efficiency of gene silencing","volume":"41","author":"Song","year":"2008","journal-title":"BMB Rep."},{"key":"2021042423511144900_BIO024943C21","first-page":"276","article-title":"Differences of glycolysis in skeletal muscle and lactate metabolism in liver between plateau zokor (Myospalax baileyi) and plateau pika (Ochotona curzoniae)","volume":"65","author":"Sun","year":"2013","journal-title":"Acta Physiol. Sinica."},{"key":"2021042423511144900_BIO024943C22","first-page":"531","article-title":"Characteristics of pulmonary acinus structure in the plateau zokor (Myospalax baileyi) and plateau pika (Ochotona curzoniae)","volume":"54","author":"Wang","year":"2008","journal-title":"J. Zool."},{"key":"2021042423511144900_BIO024943C23","first-page":"038","article-title":"Physiological character of erythrocyte adapting to hypoxia in plateau zokor and plateau pika","volume":"6","author":"Wang","year":"2008","journal-title":"Sichuan J. Zool."},{"key":"2021042423511144900_BIO024943C24","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.fob.2013.01.011","article-title":"Testis-specific lactate dehydrogenase is expressed in somatic tissues of plateau pikas","volume":"3","author":"Wang","year":"2013","journal-title":"FEBS Open Bio."},{"key":"2021042423511144900_BIO024943C25","doi-asserted-by":"publisher","first-page":"9218","DOI":"10.3390\/ijerph120809218","article-title":"Testis-specific lactate dehydrogenase (LDH-C4) in skeletal muscle enhances Apika's sprint-running capacity in hypoxic environment","volume":"12","author":"Wang","year":"2015","journal-title":"Int. J. Environ. Res. Public Health."},{"key":"2021042423511144900_BIO024943C26","doi-asserted-by":"publisher","first-page":"39","DOI":"10.3390\/ijms17010039","article-title":"Enzymatic kinetic properties of the lactate dehydrogenase isoenzyme C4 of the Plateau Pika (Ochotona curzoniae)","volume":"17","author":"Wang","year":"2016","journal-title":"Int. J. Mol. Sci."},{"key":"2021042423511144900_BIO024943C27","doi-asserted-by":"publisher","first-page":"372","DOI":"10.1016\/j.cbpa.2006.07.011","article-title":"Blood-gas properties of plateau zokor (Myospalax baileyi)","volume":"145","author":"Wei","year":"2006","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"2021042423511144900_BIO024943C28","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1042\/bj0970663","article-title":"Organ specificity and lactate-dehydrogenase activity. Some properties of human spermatozoal lactate dehydrogenase","volume":"97","author":"Wilkinson","year":"1965","journal-title":"Biochem. J."},{"key":"2021042423511144900_BIO024943C29","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1002\/j.1939-4640.2001.tb02229.x","article-title":"The role of glucose in supporting motility and capacitation in human spermatozoa","volume":"22","author":"Williams","year":"2001","journal-title":"J. Androl."},{"key":"2021042423511144900_BIO024943C30","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/S0167-4838(97)00090-3","article-title":"Selective inhibition of the sperm-specific lactate dehydrogenase isozyme-C4 by N-isopropyl oxamate","volume":"1343","author":"Wong","year":"1997","journal-title":"Biochim. Biophys. Acta"},{"key":"2021042423511144900_BIO024943C31","first-page":"373","article-title":"Difference in oxygen uptake in skeletal muscles between plateau zokor (Myospalax baileyi) and plateau pika (Ochotona curzoniac)","volume":"61","author":"Zhu","year":"2009","journal-title":"Acta Physiol. Sinica."}],"container-title":["Biology Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/bio.024943","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.logists.com\/bio\/bio\/article-pdf\/6\/9\/1336\/1832767\/bio024943.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.logists.com\/bio\/bio\/article-pdf\/6\/9\/1336\/1832767\/bio024943.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T19:49:25Z","timestamp":1719431365000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/bio\/article\/6\/9\/1336\/1876\/The-expression-of-Ldh-c-in-the-skeletal-muscle-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,15]]},"references-count":31,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9,15]]}},"URL":"https:\/\/doi.org\/10.1242\/bio.024943","relation":{},"ISSN":["2046-6390"],"issn-type":[{"value":"2046-6390","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,15]]}}}