{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T18:50:26Z","timestamp":1758394226269,"version":"3.41.2"},"reference-count":56,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":1598,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Alterations in melanocytic lineage cells give rise to a plethora of distinct human diseases, including neurocristopathies, cutaneous pigmentation disorders, loss of vision and hearing, as well as melanoma. Understanding the ontogeny and biology of melanocytic cells, as well as how they interact with their surrounding environment, are key steps in the development of therapies for diseases that involve this cell lineage. Efforts to culture and characterize primary melanocytes from normal or genetically engineered mouse models have at times yielded contrasting observations. This is due, in part, to differences in the conditions used to isolate, purify and culture these cells in individual studies. By breeding ROSAmT\/mG and Tyr::CreERT2 mice, we generated animals in which melanocytic lineage cells are identified through expression of green fluorescent protein. We also used defined conditions to systematically investigate the proliferation and migration responses of primary melanocytes on various extracellular matrix (ECM) substrates. Under our culture conditions, mouse melanocytes exhibit doubling times in the range of 10 days, and retain exponential proliferative capacity for 50-60 days. In culture, these melanocytes showed distinct responses to different ECM substrates. Specifically, laminin-332 promoted cell spreading, formation of dendrites, random motility and directional migration. In contrast, low or intermediate concentrations of collagen I promoted adhesion and acquisition of a bipolar morphology, and interfered with melanocyte forward movements. Our systematic evaluation of primary melanocyte responses emphasizes the importance of clearly defining culture conditions for these cells. This, in turn, is essential for the interpretation of melanocyte responses to extracellular cues and to understand the molecular basis of disorders involving the melanocytic cell lineage.<\/jats:p>","DOI":"10.1242\/bio.025833","type":"journal-article","created":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T00:10:15Z","timestamp":1498176615000},"source":"Crossref","is-referenced-by-count":5,"title":["A reporter mouse model for<i>in vivo<\/i>tracing and<i>in vitro<\/i>molecular studies of melanocytic lineage cells and their diseases"],"prefix":"10.1242","author":[{"given":"Melissa","family":"Crawford","sequence":"first","affiliation":[{"name":"Dept. of Physiology and Pharmacology, Children\u2019s Health Research Institute and Lawson Health Research Institute, The University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Dept. of Physiology and Pharmacology, Children\u2019s Health Research Institute and Lawson Health Research Institute, The University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1483-5159","authenticated-orcid":false,"given":"Lina","family":"Dagnino","sequence":"additional","affiliation":[{"name":"Dept. of Physiology and Pharmacology, Children\u2019s Health Research Institute and Lawson Health Research Institute, The University of Western Ontario, London, Ontario, Canada, N6A 5C1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2017,1,1]]},"reference":[{"key":"2021042423530818100_BIO025833C1","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/j.cell.2009.07.049","article-title":"Schwann cell precursors from nerve innervation are a cellular origin of melanocytes in skin","volume":"139","author":"Adameyko","year":"2009","journal-title":"Cell"},{"key":"2021042423530818100_BIO025833C2","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1111\/j.1755-148X.2007.00425.x","article-title":"Melanocyte-lineage expression of Cre recombinase using Mitf regulatory elements","volume":"21","author":"Alizadeh","year":"2008","journal-title":"Pigment Cell Melanoma Res."},{"key":"2021042423530818100_BIO025833C3","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1002\/dvg.20725","article-title":"A mart-1::Cre transgenic line induces recombination in melanocytes and retinal pigment epithelium","volume":"49","author":"Aydin","year":"2011","journal-title":"Genesis"},{"key":"2021042423530818100_BIO025833C4","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1016\/j.stem.2015.02.017","article-title":"Premigratory and migratory neural crest cells are multipotent in vivo","volume":"16","author":"Baggiolini","year":"2015","journal-title":"Cell Stem Cell"},{"key":"2021042423530818100_BIO025833C5","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1111\/j.1600-0749.2004.00147.x","article-title":"Spotlight on spotted mice: a review of white spotting mouse mutants and associated human pigmentation disorders","volume":"17","author":"Baxter","year":"2004","journal-title":"Pigment Cell Res."},{"key":"2021042423530818100_BIO025833C6","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1002\/dvg.20205","article-title":"Characterization of melanocyte-specific inducible Cre recombinase transgenic mice","volume":"44","author":"Bosenberg","year":"2006","journal-title":"Genesis"},{"key":"2021042423530818100_BIO025833C7","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1111\/j.1600-0749.2007.00387.x","article-title":"Flanking genomic region of Tyr::Cre mice, rapid genotyping for homozygous mice","volume":"20","author":"Colombo","year":"2007","journal-title":"Pigment Cell Res."},{"key":"2021042423530818100_BIO025833C8","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1038\/jid.2011.252","article-title":"Transcriptomic analysis of mouse embryonic skin cells reveals previously unreported genes expressed in melanoblasts","volume":"132","author":"Colombo","year":"2012","journal-title":"J. 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