{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:16:09Z","timestamp":1790745369648,"version":"4.1.0"},"reference-count":59,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":868,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-14-CE14-0002-01"],"award-info":[{"award-number":["ANR-14-CE14-0002-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>After entry into the host cell, the intracellular parasite Toxoplasma gondii resides within a membrane-bound compartment, the parasitophorous vacuole (PV). The PV defines an intracellular, parasite-specific niche surrounded by host organelles, including the Golgi apparatus. The mechanism by which T. gondii hijacks the host Golgi and subverts its functions remains unknown. Here, we present evidence that the dense granule protein TgGRA3 interacts with host Golgi, leading to formation of tubules and entry of host Golgi material into the PV. Targeted disruption of the TgGRA3 gene delays this engulfment of host Golgi. We also demonstrate that TgGRA3 oligomerizes and binds directly to host Golgi membranes. In addition, we show that TgGRA3 dysregulates anterograde transport in the host cell, thereby revealing one of mechanisms employed by T. gondii to recruit host organelles and divert their functions.<\/jats:p>","DOI":"10.1242\/bio.039818","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T14:44:52Z","timestamp":1551278692000},"source":"Crossref","is-referenced-by-count":26,"title":["The<i>Toxoplasma gondii<\/i>dense granule protein TgGRA3 interacts with host Golgi and dysregulates anterograde transport"],"prefix":"10.1242","author":[{"given":"Maika S.","family":"Deffieu","sequence":"first","affiliation":[{"name":"Center for Infection and Immunity of Lille, CNRS UMR 8204, INSERM U1019, Universit\u00e9 de Lille, 59 000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7291-531X","authenticated-orcid":false,"given":"Tchilabalo Dilezitoko","family":"Alayi","sequence":"additional","affiliation":[{"name":"Plateforme de Prot\u00e9omique et Peptides Modifi\u00e9s (P3M), CNRS, Universit\u00e9 de Lille, 59000 Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Slomianny","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Physiology, INSERM U 1003, Universit\u00e9 de Lille, 59655 Villeneuve d'Ascq, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2435-1684","authenticated-orcid":false,"given":"Stanislas","family":"Tomavo","sequence":"additional","affiliation":[{"name":"Plateforme de Prot\u00e9omique et Peptides Modifi\u00e9s (P3M), CNRS, Universit\u00e9 de Lille, 59000 Lille, France"},{"name":"Institute for Integrative Biology of the Cell (I2BC), CNRS UMR 9198, CEA, Universit\u00e9 Paris Sud, Universit\u00e9 Paris-Saclay, 91198 Gif-sur-Yvette Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042423392847700_BIO039818C1","doi-asserted-by":"publisher","first-page":"e1004025","DOI":"10.1371\/journal.ppat.1004025","article-title":"RON5 is critical for organization and function of the Toxoplasma moving junction complex","volume":"10","author":"Beck","year":"2014","journal-title":"PLoS Pathog."},{"key":"2021042423392847700_BIO039818C2","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1128\/EC.00081-14","article-title":"Toxoplasma gondii development of its replicative niche: in its host cell and beyond","volume-title":"Eukaryot. 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