{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:23:08Z","timestamp":1791386588833,"version":"4.3.3"},"reference-count":26,"publisher":"The Company of Biologists","issue":"5","license":[{"start":{"date-parts":[[2020,9,3]],"date-time":"2020-09-03T00:00:00Z","timestamp":1599091200000},"content-version":"vor","delay-in-days":483,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IOS-1557860"],"award-info":[{"award-number":["IOS-1557860"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000183","name":"U.S. Army Research Office","doi-asserted-by":"crossref","award":["W911NF-15-1-0175"],"award-info":[{"award-number":["W911NF-15-1-0175"]}],"id":[{"id":"10.13039\/100000183","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000006","name":"Office of Naval Research","doi-asserted-by":"publisher","award":["W911NF-16-1-0225"],"award-info":[{"award-number":["W911NF-16-1-0225"]}],"id":[{"id":"10.13039\/100000006","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100007155","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MC_U105663142"],"award-info":[{"award-number":["MC_U105663142"]}],"id":[{"id":"10.13039\/501100007155","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["110159\/Z\/15\/Z"],"award-info":[{"award-number":["110159\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect in vivo changes in mitochondrial hydrogen sulfide (H2S) in Poecilia mexicana (family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with H2S to form the product MitoN. The MitoN\/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial H2S produced over time. We describe the use of MitoA in the fish species P. mexicana to illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues followed by tandem mass spectrometry. In this proof-of-concept study we exposed H2S tolerant P. mexicana to 59\u2005\u00b5M free H2S for 5\u2005h, which resulted in increased MitoN\/MitoA in brain and gills, but not in liver or muscle, demonstrating increased mitochondrial H2S levels in select tissues following whole-animal H2S exposure. This is the first time that accumulation of H2S has been observed in vivo during whole-animal exposure to free H2S using MitoA.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/bio.041467","type":"journal-article","created":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T13:29:50Z","timestamp":1557408590000},"source":"Crossref","is-referenced-by-count":6,"title":["Detection of changes in mitochondrial hydrogen sulfide <i>i<\/i>\n                  <i>n vivo<\/i> in the fish model <i>Poecilia mexicana<\/i> (Poeciliidae)"],"prefix":"10.1242","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0448-5071","authenticated-orcid":false,"given":"Gigi Y.","family":"Lau","sequence":"first","affiliation":[{"name":"Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Barts","sequence":"additional","affiliation":[{"name":"Division of Biology, Kansas State University, 116 Ackert Hall, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard C.","family":"Hartley","sequence":"additional","affiliation":[{"name":"WestCHEM School of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Tobler","sequence":"additional","affiliation":[{"name":"Division of Biology, Kansas State University, 116 Ackert Hall, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey G.","family":"Richards","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1115-9618","authenticated-orcid":false,"given":"Michael P.","family":"Murphy","sequence":"additional","affiliation":[{"name":"MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6728-0479","authenticated-orcid":false,"given":"Sabine","family":"Arndt","sequence":"additional","affiliation":[{"name":"MRC Mitochondrial Biology Unit, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK"},{"name":"Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz, Langenbeckstr. 1, Mainz 55131, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,5,9]]},"reference":[{"key":"2021042423282747200_BIO041467C1","doi-asserted-by":"publisher","first-page":"7761","DOI":"10.1074\/jbc.M117.784678","article-title":"Assessment of H2S in vivo using the newly developed mitochondria-targeted mass spectrometry probe MitoA","volume":"292","author":"Arndt","year":"2017","journal-title":"J. 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