{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:12:04Z","timestamp":1790763124119,"version":"4.1.0"},"reference-count":42,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":868,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100013066","name":"Key Scientific Research Project of Colleges and Universities in Henan Province","doi-asserted-by":"crossref","award":["16A310001"],"award-info":[{"award-number":["16A310001"]}],"id":[{"id":"10.13039\/501100013066","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>To investigate whether exogenous hydrogen sulfide (H2S) could mitigate NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway in L02 cells. L02 cells were stimulated with different concentrations of oleic acid (OA), then cell viability and the protein expression of NLRP3 and pro-caspase-1 were detected by MTT and western blot respectively to determine appropriate OA concentration used in this study. The cells were divided into 4 groups: the cells in control group were cultured with RPMI-1640 for 24.5 h; The cells in OA group were cultured with RPMI-1640 for 0.5 h, then were stimulated with 1.2 mmol\/l OA for 24 h; The cells in NaHS+OA group were pretreated with sodium hydrogen sulfide (NaHS, a donor of H2S) 0.5 h before exposure to OA for 24 h. The cells in NaHS group were treated with NaHS 0.5 h, then were cultured with RPMI-1640 for 24 h. Subsequently the cells in every group were collected, the protein expression of NLRP3, procaspase-1, cleaved caspase-1, P62, LC3, Beclin1, T-AMPK, P-AMPK, T-mTOR, P-mTOR and the level of IL-1\u03b2 were detected by western blot and ElISA respectively. Exogenous H2S reduced the level of NLRP3, caspase-1, P62, IL-1\u03b2 and the ratio of P-mTOR\/T-mTOR induced by OA, increased the ratio of LC3 II\/I and the protein expression of Beclin1 suppressed by OA. This study demonstrated for the first time that H2S might suppress NLRP3 inflammasome-mediated inflammation induced by OA through promoting autophagy via AMPK-mTOR pathway. It provides a theoretical basis for the further study of the anti-inflammatory mechanism of H2S.<\/jats:p>","DOI":"10.1242\/bio.043653","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T13:25:12Z","timestamp":1563369912000},"source":"Crossref","is-referenced-by-count":32,"title":["Exogenous hydrogen sulfide mitigates NLRP3 inflammasome-mediated inflammation through promoting autophagy via AMPK-mTOR pathway"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7582-9102","authenticated-orcid":false,"given":"Honggang","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Biomedical Informatics, Bioinformatics Center, School of Basic Medical Sciences, Henan University, Kaifeng 475000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyu","family":"Zhong","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Informatics, Bioinformatics Center, School of Basic Medical Sciences, Henan University, Kaifeng 475000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leilei","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Informatics, Bioinformatics Center, School of Basic Medical Sciences, Henan University, Kaifeng 475000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042500064413100_BIO043653C1","doi-asserted-by":"publisher","first-page":"3402809","DOI":"10.1155\/2018\/3402809","article-title":"Hydrogen sulfide alleviates acute myocardial ischemia injury by modulating autophagy and inflammation response under oxidative stress","volume":"2018","author":"Bai","year":"2018","journal-title":"Oxid. Med. Cell Longev."},{"key":"2021042500064413100_BIO043653C2","doi-asserted-by":"publisher","first-page":"2546","DOI":"10.1074\/jbc.M117.806869","article-title":"Hydrogen sulfide inhibits the NLRP3 inflammasome and reduces cytokine production both in vitro and in a mouse model of inflammation","volume":"293","author":"Castelblanco","year":"2018","journal-title":"J. Biol. Chem."},{"key":"2021042500064413100_BIO043653C3","doi-asserted-by":"publisher","first-page":"2200","DOI":"10.1159\/000493824","article-title":"Exogenous H2S inhibits autophagy in unilateral ureteral obstruction mouse renal tubule cells by regulating the ROS-AMPK signaling pathway","volume":"49","author":"Chen","year":"2018","journal-title":"Cell. Physiol. Biochem."},{"key":"2021042500064413100_BIO043653C4","doi-asserted-by":"publisher","first-page":"6717212","DOI":"10.1155\/2018\/6717212","article-title":"Hydrogen sulfide attenuates LPS-induced acute kidney injury by inhibiting inflammation and oxidative stress","volume":"2018","author":"Chen","year":"2018","journal-title":"Oxid. Med. Cell Longev."},{"key":"2021042500064413100_BIO043653C5","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1111\/imr.12286","article-title":"Initiation and perpetuation of NLRP3 inflammasome activation and assembly","volume":"265","author":"Elliott","year":"2015","journal-title":"Immunol. Rev."},{"key":"2021042500064413100_BIO043653C6","doi-asserted-by":"publisher","first-page":"15560","DOI":"10.1073\/pnas.0705891104","article-title":"Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function","volume":"104","author":"Elrod","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042500064413100_BIO043653C7","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1007\/s00011-019-01232-0","article-title":"\u03b1-Mangostin suppresses NLRP3 inflammasome activation via promoting autophagy in LPS-stimulated murine macrophages and protects against CLP-induced sepsis in mice","volume":"68","author":"Ge","year":"2019","journal-title":"Inflamm. Res."},{"key":"2021042500064413100_BIO043653C8","doi-asserted-by":"publisher","first-page":"928","DOI":"10.1080\/15548627.2017.1293767","article-title":"Chaperone-mediated autophagy prevents cellular transformation by regulating MYC proteasomal degradation","volume":"13","author":"Gomes","year":"2017","journal-title":"Autophagy"},{"key":"2021042500064413100_BIO043653C9","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.ejphar.2014.01.009","article-title":"Hydrogen sulfide protected gastric epithelial cell from ischemia\/reperfusion injury by Keap1 s-sulfhydration, MAPK dependent anti-apoptosis and NF-kB dependent anti-inflammation pathway","volume":"725","author":"Guo","year":"2014","journal-title":"Eur. J. Pharmacol."},{"key":"2021042500064413100_BIO043653C10","doi-asserted-by":"publisher","first-page":"1578","DOI":"10.1159\/000453208","article-title":"Exogenous hydrogen sulfide attenuates high glucose-induced cardiotoxicity by inhibiting NLRP3 inflammasome activation by suppressing TLR4\/NF-\u03baB pathway in H9c2 cells","volume":"240","author":"Huang","year":"2016","journal-title":"Cell. Physiol. Biochem."},{"key":"2021042500064413100_BIO043653C11","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.exer.2018.01.004","article-title":"Hydrogen sulfide supplement attenuates the apoptosis of retinal ganglion cells in experimental glaucoma","volume":"168","author":"Huang","year":"2018","journal-title":"Exp. Eye Res."},{"key":"2021042500064413100_BIO043653C12","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/j.yexcr.2015.07.005","article-title":"PI3K\/SGK1\/GSK3\u03b2 signaling pathway is involved in inhibition of autophagy in neonatal rat cardiomyocytes exposed to hypoxia\/reoxygenation by hydrogen sulfide","volume":"345","author":"Jiang","year":"2016","journal-title":"Exp. Cell Res."},{"key":"2021042500064413100_BIO043653C13","doi-asserted-by":"publisher","first-page":"1686","DOI":"10.1002\/2211-5463.12301","article-title":"Sodium hydrosulfide attenuates cerebral ischemia\/reperfusion injury by suppressing overactivated autophagy in rats","volume":"7","author":"Jiang","year":"2017","journal-title":"FEBS Open Biol."},{"key":"2021042500064413100_BIO043653C14","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1038\/cmi.2015.95","article-title":"Molecular mechanisms regulating NLRP3 inflammasome activation","volume":"13","author":"Jo","year":"2016","journal-title":"Cell. Mol. Immunol."},{"key":"2021042500064413100_BIO043653C15","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1161\/ATVBAHA.117.310581","article-title":"Saturated fatty acids undergo intracellular crystallization and activate the NLRP3 inflammasome in macrophages","volume":"38","author":"Karasawa","year":"2018","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"2021042500064413100_BIO043653C16","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.niox.2014.01.002","article-title":"The physiological role of hydrogen sulfide and beyond","volume":"41","author":"Kimura","year":"2014","journal-title":"Nitric Oxide"},{"key":"2021042500064413100_BIO043653C17","doi-asserted-by":"publisher","first-page":"131","DOI":"10.2183\/pjab.91.131","article-title":"Hydrogen sulfide and polysulfides as signaling molecules","volume":"91","author":"Kimura","year":"2015","journal-title":"Proc. Jpn. Acad. Ser. B Phys. Biol. Sci."},{"key":"2021042500064413100_BIO043653C18","doi-asserted-by":"publisher","first-page":"452","DOI":"10.4161\/auto.4451","article-title":"Dissection of the autophagosome maturation process by a novel reporter protein, tandem fluorescent-tagged LC3","volume":"3","author":"Kimura","year":"2007","journal-title":"Autophagy"},{"key":"2021042500064413100_BIO043653C19","doi-asserted-by":"publisher","first-page":"116","DOI":"10.3389\/fnagi.2018.00116","article-title":"The NLRP3-Caspase 1 inflammasome negatively regulates autophagy via TLR4-TRIF in prion peptide-infected microglia","volume":"10","author":"Lai","year":"2018","journal-title":"Front. Aging Neurosci."},{"key":"2021042500064413100_BIO043653C20","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.bcp.2014.08.013","article-title":"Free fatty acids as modulators of the NLRP3 inflammasome in obesity\/type 2 diabetes","volume":"92","author":"Legrand-Poels","year":"2014","journal-title":"Biochem. Pharmacol."},{"key":"2021042500064413100_BIO043653C21","doi-asserted-by":"publisher","first-page":"1635","DOI":"10.1159\/000479405","article-title":"The role of exogenous hydrogen sulfide in free fatty acids induced inflammation in macrophages","volume":"42","author":"Luo","year":"2017","journal-title":"Cell. Physiol. Biochem."},{"key":"2021042500064413100_BIO043653C22","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/S1097-2765(02)00599-3","article-title":"The inflammasome:a molecular platform triggering activation of inflammatory caspases andprocessing of proIL-beta","volume":"10","author":"Martinon","year":"2002","journal-title":"Mol. Cell"},{"key":"2021042500064413100_BIO043653C23","doi-asserted-by":"publisher","first-page":"914","DOI":"10.1161\/01.RES.0000261924.76669.36","article-title":"Distinct roles of autophagy inthe heart during ischemia and reperfusion: roles of AMP-activated protein kinase and Beclin 1 in mediating autophagy","volume":"100","author":"Matsui","year":"2007","journal-title":"Circ. Res."},{"key":"2021042500064413100_BIO043653C24","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1089\/ars.2017.7261","article-title":"Autophagy attenuates angiotensin II-induced pulmonary fibrosis by inhibiting redox imbalance-mediated NLRP3 inflammasome activation","volume":"30","author":"Meng","year":"2019","journal-title":"Antioxid Redox Signal."},{"key":"2021042500064413100_BIO043653C25","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1146\/annurev-pathol-020712-163918","article-title":"Autophagy as a stress-response and quality-control mechanism: implications for cell injury and human disease","volume":"8","author":"Murrow","year":"2013","journal-title":"Annu. Rev. Pathol."},{"key":"2021042500064413100_BIO043653C26","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1089\/ars.2013.5371","article-title":"An overview of autophagy: morphology, mechanism, and regulation","volume":"20","author":"Parzych","year":"2014","journal-title":"Antioxid Redox Signal."},{"key":"2021042500064413100_BIO043653C27","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1186\/1471-2407-14-327","article-title":"The expression of beclin-1, an autophagic gene, in hepatocellular carcinoma associated with clinical pathological and prognostic significance","volume":"14","author":"Qiu","year":"2014","journal-title":"BMC Cancer"},{"key":"2021042500064413100_BIO043653C28","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1038\/nrd3802","article-title":"Autophagy modulation as a potential therapeutic target for diverse diseases","volume":"11","author":"Rubinsztein","year":"2012","journal-title":"Nat. Rev. Drug Discov."},{"key":"2021042500064413100_BIO043653C29","doi-asserted-by":"publisher","first-page":"4383","DOI":"10.1073\/pnas.0911373107","article-title":"The early autophagic pathway is activated by hepatitis B virus and required for viral DNA replication","volume":"107","author":"Sir","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042500064413100_BIO043653C30","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1016\/j.jtcvs.2008.08.074","article-title":"Hydrogen sulfide therapy attenuates the inflammatory response in a porcine model of myocardial ischemia\/reperfusion injury","volume":"138","author":"Sodha","year":"2009","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"2021042500064413100_BIO043653C31","doi-asserted-by":"publisher","first-page":"E925","DOI":"10.1152\/ajpendo.00294.2015","article-title":"Hydrogen sulfide reduces serum triglyceride by activating liver autophagy via the AMPK-mTOR pathway","volume":"309","author":"Sun","year":"2015","journal-title":"Am. J. Physiol. Endocrinol. Metab."},{"key":"2021042500064413100_BIO043653C32","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1161\/CIRCGENETICS.113.000381","article-title":"Induction of microRNA-21 with exogenous hydrogen sulfide attenuates myocardial ischemic and inflammatory injury in mice","volume":"7","author":"Toldo","year":"2014","journal-title":"Circ Cardiovasc Genet."},{"key":"2021042500064413100_BIO043653C33","first-page":"1233","article-title":"Endogenous cystathionine-gamma-lyase\/hydrogen sulfide pathway regulates apoptosis of HepG2 cells","volume":"48","author":"Wang","year":"2013","journal-title":"Yao Xue Xue Bao."},{"key":"2021042500064413100_BIO043653C34","doi-asserted-by":"publisher","first-page":"e2688","DOI":"10.1038\/cddis.2017.18","article-title":"Hydrogen sulfide promotes autophagy of hepatocellular carcinoma cells through the PI3K\/Akt\/mTOR signaling pathway","volume":"8","author":"Wang","year":"2017","journal-title":"Cell Death Dis."},{"key":"2021042500064413100_BIO043653C35","doi-asserted-by":"publisher","first-page":"858","DOI":"10.3389\/fphar.2018.00858","article-title":"Hydrogen sulfide ameliorates blood-spinal cord barrier disruption and improves functional recovery by inhibiting endoplasmic reticulum stress-dependent autophagy","volume":"9","author":"Wang","year":"2018","journal-title":"Front. Pharmacol."},{"key":"2021042500064413100_BIO043653C36","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1111\/cns.12005","article-title":"A double-edged sword with therapeutic potential: an updated role of autophagy in ischemic cerebral injury","volume":"18","author":"Wei","year":"2012","journal-title":"CNS Neurosci. Ther."},{"key":"2021042500064413100_BIO043653C37","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1038\/s41598-017-00557-2","article-title":"Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS\/MAPK and NF-\u03baB signaling pathways","volume":"7","author":"Wu","year":"2017","journal-title":"Sci. Rep."},{"key":"2021042500064413100_BIO043653C38","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1007\/s11010-019-03519-6","article-title":"Exogenous hydrogen sulfide mitigates LPS+ATP-induced inflammation by inhibiting NLRP3 inflammasome activation and promoting autophagy in L02 cells","volume":"457","author":"Wu","year":"2019","journal-title":"Mol. Cell. Biochem."},{"key":"2021042500064413100_BIO043653C39","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.cbi.2018.02.028","article-title":"NaHS restores mitochondrial function and inhibits autophagy by activating the PI3K\/Akt\/mTOR signalling pathway to improve functional recovery after traumatic brain injury","volume":"286","author":"Xu","year":"2018","journal-title":"Chem. Biol. Interact."},{"key":"2021042500064413100_BIO043653C40","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1038\/s41418-018-0105-8","article-title":"lincRNA-Cox2 regulates NLRP3 inflammasome and autophagy mediated neuroinflammation","volume":"26","author":"Xue","year":"2019","journal-title":"Cell Death Differ."},{"key":"2021042500064413100_BIO043653C41","doi-asserted-by":"publisher","first-page":"2093","DOI":"10.1007\/s00204-018-2208-x","article-title":"Activation of autophagic flux and the Nrf2\/ARE signaling pathway by hydrogen sulfide protects against acrylonitrile-induced neurotoxicity in primary rat astrocytes","volume":"92","author":"Yang","year":"2018","journal-title":"Arch. Toxicol."},{"key":"2021042500064413100_BIO043653C42","doi-asserted-by":"publisher","first-page":"9647809","DOI":"10.1155\/2018\/9647809","article-title":"Hydrogen sulfide alleviates lipopolysaccharide-induced diaphragm dysfunction in rats by reducing apoptosis and inflammation through ROS\/MAPK and TLR4\/NF-\u03baB signaling pathways","volume":"2018","author":"Zhang","year":"2018","journal-title":"Oxid. Med. Cell Longev."}],"container-title":["Biology Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/bio.043653","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.logists.com\/bio\/bio\/article-pdf\/doi\/10.1242\/bio.043653\/1830493\/bio043653.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.logists.com\/bio\/bio\/article-pdf\/doi\/10.1242\/bio.043653\/2061434\/bio_043653v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.logists.com\/bio\/bio\/article-pdf\/doi\/10.1242\/bio.043653\/2061434\/bio_043653v1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T23:58:20Z","timestamp":1621382300000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/bio\/article\/doi\/10.1242\/bio.043653\/258511\/Exogenous-hydrogen-sulfide-mitigates-NLRP3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,1]]},"references-count":42,"URL":"https:\/\/doi.org\/10.1242\/bio.043653","relation":{},"ISSN":["2046-6390"],"issn-type":[{"value":"2046-6390","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,1]]},"article-number":"bio.043653"}}