{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T01:38:54Z","timestamp":1780105134418,"version":"3.54.0"},"reference-count":86,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201408080114"],"award-info":[{"award-number":["201408080114"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["BI 1551\/3-1"],"award-info":[{"award-number":["BI 1551\/3-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>VEGF is a crucial stimulator for choroidal neovascularization (CNV). Our aim was to develop a reproducible and valid treatment-naive quiescent CNV (i.e. without signs of exudation and with normal visual acuity) rat model by subretinal injection of an adeno-associated virus (AAV)-VEGFA165 vector. The CNV development was longitudinally followed up in vivo by scanning laser ophthalmoscopy\/optical coherence tomography, fluorescein and indocyanine green angiographies and ex vivo by electron microscopy (EM) and immunohistochemistry. In total, 57 eyes were analysed. In vivo, a quiescent CNV was observed in 93% of the eyes six weeks post-transduction. In EM, CNV vessels with few fenestrations, multi-layered basement membranes, and bifurcation of endothelial cells were observed sharing the human CNV features. Human VEGF overexpression, multi-layered RPE (RPE65) and macrophages\/activated microglia (Iba1) were also detected. In addition, 19 CNV eyes were treated up to three weeks with bevacizumab. The retinal and the CNV lesion thickness decreased significantly in bevacizumab-treated CNV eyes compared to untreated CNV eyes one week after the treatment.<\/jats:p>\n               <jats:p>In conclusion, our experimental CNV resembles those seen in patients suffering from treatment-naive quiescent CNV in wet-age related macular degeneration, and responses to short-term treatment with bevacizumab. Our new model can, therefore, be used to test the long-term effect of new drugs targeting CNV under precisely defined conditions.<\/jats:p>","DOI":"10.1242\/bio.048736","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T18:25:17Z","timestamp":1582309517000},"source":"Crossref","is-referenced-by-count":11,"title":["A new rat model of treatment-naive quiescent choroidal neovascularization induced by human VEGF165 overexpression"],"prefix":"10.1242","author":[{"given":"Shan","family":"Liu","sequence":"first","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antje K.","family":"Biesemeier","sequence":"additional","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"},{"name":"Natural and Medical Institute at the University of Tuebingen, Applied Material Science and Electron Microscopy, Reutlingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander V.","family":"Tschulakow","sequence":"additional","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"},{"name":"STZ OcuTox Preclinical Drug Assessment, Hechingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsh V.","family":"Thakkar","sequence":"additional","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"},{"name":"STZ OcuTox Preclinical Drug Assessment, Hechingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Julien-Schraermeyer","sequence":"additional","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"},{"name":"STZ OcuTox Preclinical Drug Assessment, Hechingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7969-8426","authenticated-orcid":false,"given":"Ulrich","family":"Schraermeyer","sequence":"additional","affiliation":[{"name":"Center for Ophthalmology, Division of experimental vitreoretinal surgery, Tuebingen, Germany"},{"name":"STZ OcuTox Preclinical Drug Assessment, Hechingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042622461619100_BIO048736C1","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.2147\/DDDT.S103993","article-title":"Prophylactic effects of Clausena excavata Burum. f. leaf extract in ethanol-induced gastric ulcers","volume":"10","author":"Albaayit","year":"2016","journal-title":"Drug Des. 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