{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:17:12Z","timestamp":1790234232507,"version":"4.1.0"},"reference-count":38,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23500477"],"award-info":[{"award-number":["23500477"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007428","name":"Naito Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Calpain-3 (CAPN3) is a muscle specific type of calpain whose protease activity is triggered by Ca2+. Here, we developed CAPN3 sensor probes (SPs) to detect activated-CAPN3 using a fluorescence\/F\u00f6rster resonance energy transfer (FRET) technique. In our SPs, partial amino acid sequence of calpastatin, endogenous CAPN inhibitor but CAPN3 substrate, is inserted between two different fluorescence proteins which cause FRET. Biochemical and spectral studies revealed that CAPN3 cleaved SPs and changed emission wavelengths of SPs. Importantly, SPs were scarcely cleaved by CAPN1 and CAPN2. Furthermore, our SP successfully captured the activation of endogenous CAPN3 in living myotubes treated with ouabain. Our SPs would become a promising tool to detect the dynamics of CAPN3 protease activity in living cells.<\/jats:p>","DOI":"10.1242\/bio.048975","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T13:51:41Z","timestamp":1597413101000},"source":"Crossref","is-referenced-by-count":5,"title":["Developing fluorescence sensor probe to capture activated muscle-specific calpain-3 (CAPN3) in living muscle cells"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5973-1073","authenticated-orcid":false,"given":"Koichi","family":"Ojima","sequence":"first","affiliation":[{"name":"Muscle Biology Research Unit, Division of Animal Products Research, Institute of Livestock and Grassland Science, NARO, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoji","family":"Hata","sequence":"additional","affiliation":[{"name":"Calpain Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiko","family":"Shinkai-Ouchi","sequence":"additional","affiliation":[{"name":"Calpain Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mika","family":"Oe","sequence":"additional","affiliation":[{"name":"Muscle Biology Research Unit, Division of Animal Products Research, Institute of Livestock and Grassland Science, NARO, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Muroya","sequence":"additional","affiliation":[{"name":"Muscle Biology Research Unit, Division of Animal Products Research, Institute of Livestock and Grassland Science, NARO, Tsukuba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Sorimachi","sequence":"additional","affiliation":[{"name":"Calpain Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Ono","sequence":"additional","affiliation":[{"name":"Calpain Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042700321450900_BIO048975C1","doi-asserted-by":"publisher","first-page":"39672","DOI":"10.1074\/jbc.M608803200","article-title":"A mouse model for monitoring calpain activity under physiological and pathological conditions","volume":"281","author":"Bartoli","year":"2006","journal-title":"J. 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