{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:28:16Z","timestamp":1790645296808,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["I80VP000003"],"award-info":[{"award-number":["I80VP000003"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Store-operated Ca2+ entry (SOCE) is an essential Ca2+ signaling mechanism present in most animal cells. SOCE refers to Ca2+ influx that is activated by depletion of sarco\/endoplasmic reticulum (S\/ER) Ca2+ stores. The main components of SOCE are STIM and Orai. STIM proteins function as S\/ER Ca2+ sensors, and upon S\/ER Ca2+ depletion STIM rearranges to S\/ER-plasma membrane junctions and activates Orai Ca2+ influx channels. Studies have implicated SOCE in cardiac hypertrophy pathogenesis, but SOCE's role in normal heart physiology remains poorly understood. We therefore analyzed heart-specific SOCE function in Drosophila, a powerful animal model of cardiac physiology. We show that heart-specific suppression of Stim and Orai in larvae and adults resulted in reduced contractility consistent with dilated cardiomyopathy. Myofibers were also highly disorganized in Stim and Orai RNAi hearts, reflecting possible decompensation or upregulated stress signaling. Furthermore, we show that reduced heart function due to SOCE suppression adversely affected animal viability, as heart specific Stim and Orai RNAi animals exhibited significant delays in post-embryonic development and adults died earlier than controls. Collectively, our results demonstrate that SOCE is essential for physiological heart function, and establish Drosophila as an important model for understanding the role of SOCE in cardiac pathophysiology.<\/jats:p>","DOI":"10.1242\/bio.049999","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T13:25:29Z","timestamp":1582291529000},"source":"Crossref","is-referenced-by-count":15,"title":["Suppression of store-operated calcium entry causes dilated cardiomyopathy of the\n                    <i>Drosophila<\/i>\n                    heart"],"prefix":"10.1242","author":[{"given":"Courtney E.","family":"Petersen","sequence":"first","affiliation":[{"name":"Graduate Program in Molecular and Cellular Biology, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine, Department of Medicine, The University of Virginia School of Medicine, Charlottesville, VA 22908, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7809-2193","authenticated-orcid":false,"given":"Jeremy T.","family":"Smyth","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, F. Edward H\u00e9bert School of Medicine, Bethesda, MD 20814, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042622420426000_BIO049999C1","doi-asserted-by":"publisher","first-page":"e77785","DOI":"10.1371\/journal.pone.0077785","article-title":"Disruption of sarcoendoplasmic reticulum calcium ATPase function in Drosophila leads to cardiac dysfunction","volume":"8","author":"Abraham","year":"2013","journal-title":"PLoS ONE"},{"key":"2021042622420426000_BIO049999C2","doi-asserted-by":"publisher","first-page":"e1423","DOI":"10.3791\/1423","article-title":"Fluorescent labeling of Drosophila heart structures","volume":"32","author":"Alayari","year":"2009","journal-title":"J. Vis. 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