{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:40:36Z","timestamp":1764700836268,"version":"3.41.2"},"reference-count":27,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Hypertrophic scar (HS) is a dermal fibro-proliferative disorder result from abnormal wound healing after skin injury. MicroRNA-9-5p (miR-9-5p) has been reported to be upregulated and closely related to collagen proteins in human dermal fibroblasts. However, the correlation and possible mechanism between miR-9-5p and HS require further investigation. The expressions of miR-9-5p in HS tissues and HS fibroblasts were detected by quantitative real-time PCR (RT-qPCR). The expression level of peroxisome proliferator-activated receptor \u03b2 (PPAR\u03b2) was measured by RT-qPCR assay. The protein levels of PPAR\u03b2, \u03b1-SMA, Vimentin, COL1A, cyclin D1, bcl-2, and bax were detected by western blot assay. The effect of miR-9-5p and PPAR\u03b2 on HS fibroblasts proliferation and apoptosis were detected by cell counting kit-8 (CCK-8) and flow cytometry assays. The interaction between miR-9-5p and PPAR\u03b2 was predicted by TargetScan, and then confirmed by dual-luciferase reporter assay. MiR-9-5p expression was downregulated in HS tissues and HS fibroblasts. MiR-9-5p inhibited the levels of extracellular matrix-associated genes (\u03b1-SMA, Vimentin, COL1A) in HS fibroblasts. MiR-9-5p repressed proliferation and induced apoptosis of HS fibroblasts. PPAR\u03b2 is a target gene of miR-9-5p. The silencing of PPAR\u03b2 expression hindered proliferation and expedited apoptosis of HS fibroblasts. MiR-9-5p suppressed proliferation and promoted apoptosis of HS fibroblasts by targeting PPAR\u03b2. In this paper, we firstly disclosed that miR-9-5p hampered extracellular matrix deposition and proliferation, and induced apoptosis by targeting PPAR\u03b2 in HS fibroblasts. Our findings provided a new role of miR-9-5p\/PPAR\u03b2 in the occurrence and development of HS fibroblasts, promising a new target for HS.<\/jats:p>","DOI":"10.1242\/bio.051904","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T23:14:15Z","timestamp":1608678855000},"source":"Crossref","is-referenced-by-count":13,"title":["MicroRNA-9-5p inhibits proliferation and induces apoptosis of human hypertrophic scar fibroblasts through targeting peroxisome proliferator-activated receptor \u03b2"],"prefix":"10.1242","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3612-8711","authenticated-orcid":false,"given":"Chi-Yung","family":"Chai","sequence":"first","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5107-016X","authenticated-orcid":false,"given":"I.-Chun","family":"Tai","sequence":"additional","affiliation":[{"name":"Reichen Biomedical Co., Ltd., Kaohsiung, Taiwan 81155, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4333-4137","authenticated-orcid":false,"given":"Rui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oncology Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233003, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6977-5766","authenticated-orcid":false,"given":"Junlong","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6624-3860","authenticated-orcid":false,"given":"Chaoying","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0796-7049","authenticated-orcid":false,"given":"Shengrong","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2020,12,21]]},"reference":[{"key":"2021042700291211700_BIO051904C1","doi-asserted-by":"publisher","first-page":"e234","DOI":"10.1371\/journal.pmed.0040234","article-title":"Hypertrophic scar formation following burns and trauma: new approaches to treatment","volume":"4","author":"Aarabi","year":"2007","journal-title":"PLoS Med."},{"key":"2021042700291211700_BIO051904C2","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s40659-017-0127-6","article-title":"MicroRNA-98 inhibits the cell proliferation of human hypertrophic scar fibroblasts via targeting Col1A1","volume":"50","author":"Bi","year":"2017","journal-title":"Biol. 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