{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:57:03Z","timestamp":1790704623257,"version":"4.1.0"},"reference-count":52,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T00:00:00Z","timestamp":1612828800000},"content-version":"vor","delay-in-days":239,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["18-00514S"],"award-info":[{"award-number":["18-00514S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Charles University Grant Agency","doi-asserted-by":"publisher","award":["1034120"],"award-info":[{"award-number":["1034120"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Czech Centre for Phenogenomics","award":["LM2015040"],"award-info":[{"award-number":["LM2015040"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Cranial neural crest cells (cNCCs) originate in the anterior neural tube and populate pharyngeal arches in which they contribute to formation of bone and cartilage. This cell population also provides molecular signals for the development of tissues of non-neural crest origin, such as the tongue muscles, teeth enamel or gland epithelium. Here we show that the transcription factor Meis2 is expressed in the oral region of the first pharyngeal arch (PA1) and later in the tongue primordium. Conditional inactivation of Meis2 in cNCCs resulted in loss of Sonic hedgehog signalling in the oropharyngeal epithelium and impaired patterning of PA1 along the lateral\u2013medial and oral\u2013aboral axis. Failure of molecular specification of PA1, illustrated by altered expression of Hand1\/2, Dlx5, Barx1, Gsc and other markers, led to hypoplastic tongue and ectopic ossification of the mandible. Meis2-mutant mice thus display craniofacial defects that are reminiscent of several human syndromes and patients with mutations in the Meis2 gene.<\/jats:p>","DOI":"10.1242\/bio.052043","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T13:39:36Z","timestamp":1593697176000},"source":"Crossref","is-referenced-by-count":29,"title":["Neural crest cells require Meis2 for patterning the mandibular arch via the Sonic hedgehog pathway"],"prefix":"10.1242","volume":"9","author":[{"given":"Jaroslav","family":"Fabik","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Institute of Experimental Medicine of the Czech Academy of Sciences, Praha, Czech Republic"},{"name":"Department of Cell Biology, Faculty of Science, Charles University, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Kovacova","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Institute of Experimental Medicine of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5850-2105","authenticated-orcid":false,"given":"Zbynek","family":"Kozmik","sequence":"additional","affiliation":[{"name":"Laboratory of Transcriptional Regulation, Institute of Molecular Genetics of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-1406","authenticated-orcid":false,"given":"Ondrej","family":"Machon","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Institute of Experimental Medicine of the Czech Academy of Sciences, Praha, Czech Republic"},{"name":"Laboratory of Transcriptional Regulation, Institute of Molecular Genetics of the Czech Academy of Sciences, Praha, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,7,2]]},"reference":[{"key":"2021042622464686200_BIO052043C1","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1242\/dev.097295","article-title":"Meis2 is a Pax6 co-factor in neurogenesis and dopaminergic periglomerular fate specification in the adult olfactory bulb","volume":"141","author":"Agoston","year":"2014","journal-title":"Development"},{"key":"2021042622464686200_BIO052043C2","doi-asserted-by":"publisher","first-page":"e1006441","DOI":"10.1371\/journal.pgen.1006441","article-title":"The gene regulatory network of lens induction is wired through meis-dependent shadow enhancers of Pax6","volume":"12","author":"Antosova","year":"2016","journal-title":"PLoS Genet."},{"key":"2021042622464686200_BIO052043C3","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1016\/j.stem.2015.02.017","article-title":"Premigratory and migratory neural crest cells are multipotent in vivo","volume":"16","author":"Baggiolini","year":"2015","journal-title":"Cell Stem Cell"},{"key":"2021042622464686200_BIO052043C4","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1242\/dev.056929","article-title":"Downregulation of Dlx5 and Dlx6 expression by Hand2 is essential for initiation of tongue morphogenesis","volume":"138","author":"Barron","year":"2011","journal-title":"Development"},{"key":"2021042622464686200_BIO052043C5","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1002\/dvdy.24256","article-title":"Sonic hedgehog from pharyngeal arch 1 epithelium is necessary for early mandibular arch cell survival and later cartilage condensation differentiation","volume":"244","author":"Billmyre","year":"2015","journal-title":"Dev. 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