{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T19:53:26Z","timestamp":1789070006003,"version":"build-2803163510"},"reference-count":59,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003170","name":"Stiftelsen f\u00f6r Kunskaps- och Kompetensutveckling","doi-asserted-by":"crossref","award":["2014\/0301"],"award-info":[{"award-number":["2014\/0301"]}],"id":[{"id":"10.13039\/501100003170","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Cardiac hypertrophy is an important and independent risk factor for the development of heart failure. To better understand the mechanisms and regulatory pathways involved in cardiac hypertrophy, there is a need for improved in vitro models. In this study, we investigated how hypertrophic stimulation affected human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (CMs). The cells were stimulated with endothelin-1 (ET-1) for 8, 24, 48, 72, or 96h. Parameters including cell size, ANP-, proBNP-, and lactate concentration were analyzed. Moreover, transcriptional profiling using RNA-sequencing was performed to identify differentially expressed genes following ET-1 stimulation. The results show that the CMs increase in size by approximately 13% when exposed to ET-1 in parallel to increases in ANP and proBNP protein and mRNA levels. Furthermore, the lactate concentration in the media was increased indicating that the CMs consume more glucose, a hallmark of cardiac hypertrophy. Using RNA-seq, a hypertrophic gene expression pattern was also observed in the stimulated CMs. Taken together, these results show that hiPSC-derived CMs stimulated with ET-1 display a hypertrophic response. The results from this study also provide new molecular insights about the underlying mechanisms of cardiac hypertrophy and may help accelerate the development of new drugs against this condition.<\/jats:p>","DOI":"10.1242\/bio.052381","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T19:55:26Z","timestamp":1599076526000},"source":"Crossref","is-referenced-by-count":19,"title":["Cardiac hypertrophy in a dish: A human stem cell based model"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5134-4749","authenticated-orcid":false,"given":"Markus","family":"Johansson","sequence":"first","affiliation":[{"name":"Systems Biology Research Center, School of Bioscience, University of Sk\u00f6vde, SE-541 28 Sk\u00f6vde, Sweden"},{"name":"Department of Molecular and Clinical Medicine, Institute of Medicine, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9242-4852","authenticated-orcid":false,"given":"Benjamin","family":"Ulfenborg","sequence":"additional","affiliation":[{"name":"Systems Biology Research Center, School of Bioscience, University of Sk\u00f6vde, SE-541 28 Sk\u00f6vde, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian X.","family":"Andersson","sequence":"additional","affiliation":[{"name":"Takara Bio Europe AB, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sepideh","family":"Heydarkhan-Hagvall","sequence":"additional","affiliation":[{"name":"Systems Biology Research Center, School of Bioscience, University of Sk\u00f6vde, SE-541 28 Sk\u00f6vde, Sweden"},{"name":"Bioscience, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals, R&D AstraZeneca, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Jeppsson","sequence":"additional","affiliation":[{"name":"Department of Molecular and Clinical Medicine, Institute of Medicine, The Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden"},{"name":"Department of Cardiothoracic Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Sartipy","sequence":"additional","affiliation":[{"name":"Systems Biology Research Center, School of Bioscience, University of Sk\u00f6vde, SE-541 28 Sk\u00f6vde, Sweden"},{"name":"Late-stage Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4697-0590","authenticated-orcid":false,"given":"Jane","family":"Synnergren","sequence":"additional","affiliation":[{"name":"Systems Biology Research Center, School of Bioscience, University of Sk\u00f6vde, SE-541 28 Sk\u00f6vde, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042700330559200_BIO052381C1","doi-asserted-by":"publisher","first-page":"e108051","DOI":"10.1371\/journal.pone.0108051","article-title":"RNA expression profiling of human iPSC-derived cardiomyocytes in a cardiac hypertrophy model","volume":"9","author":"Aggarwal","year":"2014","journal-title":"PLoS ONE"},{"key":"2021042700330559200_BIO052381C2","first-page":"327","article-title":"B-type natriuretic peptide and left ventricular hypertrophy in hypertensive patients","volume":"22","author":"Almeida","year":"2003","journal-title":"Rev. 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