{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T05:02:23Z","timestamp":1772168543829,"version":"3.50.1"},"reference-count":24,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:00:00Z","timestamp":1621296000000},"content-version":"am","delay-in-days":503,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"funder-list","award":["CF 319"],"award-info":[{"award-number":["CF 319"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The nematode worm Caenorhabditis elegans depends on microbes in decaying vegetation as its food source. To survive in an environment rich in opportunistic pathogens, C. elegans has evolved an epithelial defence system where surface-exposed tissues such as epidermis, pharynx, intestine, vulva and hindgut have the capacity of eliciting appropriate immune defences to acute gut infection. However, it is unclear how the worm responds to chronic intestinal infections. To this end, we have surveyed C. elegans mutants that are involved in inflammation, immunity and longevity to find their phenotypes during chronic infection. Worms that grew in a monoculture of the natural pathogen Microbacterium nematophilum (CBX102 strain) had a reduced lifespan and vigour. This was independent of intestinal colonisation as both CBX102 and the derived avirulent strain UV336 were early persistent colonisers. In contrast, the long-lived daf-2 mutant was resistant to chronic infection, showing reduced colonisation and higher vigour. In fact, UV336 interaction with daf-2, resulted in a host lifespan extension beyond OP50, the E. coli strain used for laboratory C. elegans culture. Longevity and vigour of daf-2 mutants growing on CBX102 was dependent on the FOXO orthologue DAF-16. Our results indicate that the interaction between host genotype and strain-specific bacteria determines longevity and health for C. elegans.<\/jats:p>","DOI":"10.1242\/bio.053504","type":"journal-article","created":{"date-parts":[[2020,6,24]],"date-time":"2020-06-24T13:04:36Z","timestamp":1593003876000},"source":"Crossref","is-referenced-by-count":0,"title":["From pathogen to a commensal: modification of the\n                    <i>Microbacterium nematophilum-C. elegans<\/i>\n                    interaction during chronic infection by the absence of host insulin signalling"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8032-2565","authenticated-orcid":false,"given":"Maria","family":"Gravato-Nobre","sequence":"first","affiliation":[{"name":"Laboratory of Cell Biology, Development and Genetics, Department of Biochemistry, University of Oxford, South Parks Rd OX1 3QU Oxford UK"},{"name":"Present address: MoA Technology, BioEscalator, University of Oxford, Innovation Building, Oxford OX3 7FZ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1079-7661","authenticated-orcid":false,"given":"Jonathan","family":"Hodgkin","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Development and Genetics, Department of Biochemistry, University of Oxford, South Parks Rd OX1 3QU Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9498-9993","authenticated-orcid":false,"given":"Petros","family":"Ligoxygakis","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Biology, Development and Genetics, Department of Biochemistry, University of Oxford, South Parks Rd OX1 3QU Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2020,1,1]]},"reference":[{"key":"2021042622425019300_BIO053504C1","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1111\/j.1574-6968.2006.00469.x","article-title":"Genome characterization, analysis of virulence and transformation of Microbacterium nematophilum, a coryneform pathogen of the nematode Caenorhabditis elegans","volume":"264","author":"Akimkina","year":"2006","journal-title":"FEMS Microbiol. 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