{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:12:41Z","timestamp":1765807961124,"version":"3.41.2"},"reference-count":47,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T00:00:00Z","timestamp":1627257600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2017-051196","RGPIN-2020-06102"],"award-info":[{"award-number":["RGPIN-2017-051196","RGPIN-2020-06102"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Claudin (Cldn)-10 tight junction (TJ) proteins are hypothesized to form the paracellular Na+ secretion pathway of hyposmoregulating mummichog (Fundulus heteroclitus) branchial epithelia. Organ-specific expression profiles showed that only branchial organs [the gill and opercular epithelium (OE)] exhibited abundant cldn-10 paralog transcripts, which typically increased following seawater (SW) to hypersaline (2SW) challenge. Post-translational properties, protein abundance, and ionocyte localization of Cldn-10c, were then examined in gill and OE. Western blot analysis revealed two Cldn-10c immunoreactive bands in the mummichog gill and OE at \u223c29\u2005kDa and \u223c40\u2005kDa. The heavier protein could be eliminated by glycosidase treatment, demonstrating the novel presence of a glycosylated Cldn-10c. Protein abundance of Cldn-10c increased in gill and OE of 2SW-exposed fish. Cldn-10c localized to the sides of gill and OE ionocyte apical crypts and partially colocalized with cystic fibrosis transmembrane conductance regulator and F-actin, consistent with TJ complex localization. Cldn-10c immunofluorescent intensity increased but localization was unaltered by 2SW conditions. In support of our hypothesis, cldn-10\/Cldn-10 TJ protein dynamics in gill and OE of mummichogs and TJ localization are functionally consistent with the creation and maintenance of salinity-responsive, cation-selective pores that facilitate Na+ secretion in hyperosmotic environments.<\/jats:p>","DOI":"10.1242\/bio.058868","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T11:33:53Z","timestamp":1624361633000},"source":"Crossref","is-referenced-by-count":6,"title":["Mummichog gill and operculum exhibit functionally consistent <i>claudin-10<\/i> paralog profiles and Claudin-10c hypersaline response"],"prefix":"10.1242","volume":"10","author":[{"given":"Chun Chih","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Biology, York University, Toronto, ON M3J 1P3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George N.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina R. F.","family":"Cozzi","sequence":"additional","affiliation":[{"name":"Department of Biology, St. Francis Xavier University, Antigonish, NS B2G 2W5, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5421-873X","authenticated-orcid":false,"given":"Scott P.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Biology, York University, Toronto, ON M3J 1P3, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"2021072608083488400_BIO058868C1","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.cbpa.2013.11.004","article-title":"Cold acclimation of NaCl secretion in a eurythermic teleost: mitochondrial function and gill remodeling","volume":"168","author":"Barnes","year":"2014","journal-title":"Comp. Biochem. Physiol. A Molec. Integ. 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