{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:37:34Z","timestamp":1790753854041,"version":"4.1.0"},"reference-count":28,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T00:00:00Z","timestamp":1662940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Macy Easom Cancer Research Foundation Grant"},{"name":"Baylor College of Medicine Michael E. DeBakey Department of Surgery Faculty Research Award"},{"name":"U.S. Department of Defense Career Development Award","award":["W81XWH-21-1-0396"],"award-info":[{"award-number":["W81XWH-21-1-0396"]}]},{"name":"Cancer Prevention and Research Institute of Texas (CPRIT) Multi-Investigator Research Award","award":["RP180674"],"award-info":[{"award-number":["RP180674"]}]},{"name":"NIH"},{"name":"CPRIT Core Facility Support Award","award":["CPRIT-RP180672"],"award-info":[{"award-number":["CPRIT-RP180672"]}]},{"name":"NIH","award":["P30 CA125123"],"award-info":[{"award-number":["P30 CA125123"]}]},{"name":"NIH","award":["S10 RR024574"],"award-info":[{"award-number":["S10 RR024574"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Hepatoblastoma (HB) is the most common pediatric primary liver malignancy, and survival for high-risk disease approaches 50%. Mouse models of HB fail to recapitulate hallmarks of high-risk disease. The aim of this work was to generate murine models that show high-risk features including multifocal tumors, vascular invasion, metastasis, and circulating tumor cells (CTCs). HepT1 cells were injected into the livers or tail veins of mice, and tumor growth was monitored with magnetic resonance and bioluminescent imaging. Blood was analyzed with fluorescence-activated cell sorting to identify CTCs. Intra- and extra-hepatic tumor samples were harvested for immunohistochemistry and RNA and DNA sequencing. Cell lines were grown from tumor samples and profiled with RNA sequencing. With intrahepatic injection of HepT1 cells, 100% of animals grew liver tumors and showed vascular invasion, metastasis, and CTCs. Mutation profiling revealed genetic alterations in seven cancer-related genes, while transcriptomic analyses showed changes in gene expression with cells that invade vessels. Tail vein injection of HepT1 cells resulted in multifocal, metastatic disease. These unique models will facilitate further meaningful studies of high-risk HB.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/bio.058973","type":"journal-article","created":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T07:51:32Z","timestamp":1650613892000},"source":"Crossref","is-referenced-by-count":5,"title":["HepT1-derived murine models of high-risk hepatoblastoma display vascular invasion, metastasis, and circulating tumor cells"],"prefix":"10.1242","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0515-5795","authenticated-orcid":false,"given":"Sarah E.","family":"Woodfield","sequence":"first","affiliation":[{"name":"Dan L. Duncan Cancer Center, Baylor College of Medicine 1 Divisions of Pediatric Surgery and Surgical Research, Michael E. 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