{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:20:27Z","timestamp":1790446827120,"version":"4.1.0"},"reference-count":42,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for Promotion of Science","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012319","name":"National Center for Global Health and Medicine","doi-asserted-by":"crossref","award":["30-1006"],"award-info":[{"award-number":["30-1006"]}],"id":[{"id":"10.13039\/100012319","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012319","name":"National Center for Global Health and Medicine","doi-asserted-by":"crossref","award":["20A1007"],"award-info":[{"award-number":["20A1007"]}],"id":[{"id":"10.13039\/100012319","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100012319","name":"National Center for Global Health and Medicine","doi-asserted-by":"crossref","award":["SRP#2019-9"],"award-info":[{"award-number":["SRP#2019-9"]}],"id":[{"id":"10.13039\/100012319","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Tokyo University of Agriculture and Technology: Tokyo Noko Daigaku"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The tuft cell is a chemosensory cell, a specific cell type sharing the taste transduction system with a taste cell on the tongue, of which the existence has been discovered in various tissues including the gastrointestinal tract, gall bladder, trachea and pancreatic duct. To date, electron microscopic approaches have shown various morphological features of the tuft cell, such as long and thick microvilli, tubulovesicular network at the apical side and prominent skeleton structures. Recently, it has been reported that the small intestinal tuft cell functions to initiate type-2 immunity in response to helminth infection. However, the mechanisms by which such distinguished structures are involved with the physiological functions are poorly understood. To address this question, a combination of physiological study of tuft cells using genetic models and its morphological study using electron microscopy will be required. However, it is a challenge to observe tuft cells by electron microscopy due to their extremely low frequency in the epithelium. Therefore, in this paper, we suggest an advanced protocol to observe the small intestinal tuft cell efficiently by transmission electron microscopy using serial semi-thin sections on Aclar film.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/bio.059007","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T05:03:01Z","timestamp":1641531781000},"source":"Crossref","is-referenced-by-count":9,"title":["Novel protocol to observe the intestinal tuft cell using transmission electron microscopy"],"prefix":"10.1242","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0253-9416","authenticated-orcid":false,"given":"Takuma","family":"Kozono","sequence":"first","affiliation":[{"name":"Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"},{"name":"Department of Gastroenterology, The Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba 272-8516, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8502-6474","authenticated-orcid":false,"given":"Miwa","family":"Tamura-Nakano","sequence":"additional","affiliation":[{"name":"Communal Laboratory, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki I.","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The Research Center for Hepatitis and Immunology, Research Institute, National Center for Global Health and Medicine, Chiba 272-8516, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5422-7548","authenticated-orcid":false,"given":"Takashi","family":"Tonozuka","sequence":"additional","affiliation":[{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4325-1160","authenticated-orcid":false,"given":"Atsushi","family":"Nishikawa","sequence":"additional","affiliation":[{"name":"Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"},{"name":"Department of Applied Biological Chemistry, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"2022042613513404900_BIO059007C1","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1053\/j.gastro.2013.09.050","article-title":"DCLK1 marks a morphologically distinct subpopulation of cells with stem cell properties in preinvasive pancreatic cancer","volume":"146","author":"Bailey","year":"2014","journal-title":"Gastroenterology"},{"key":"2022042613513404900_BIO059007C2","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1038\/s41586-018-0346-1","article-title":"Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells","volume":"559","author":"Bornstein","year":"2018","journal-title":"Nature"},{"key":"2022042613513404900_BIO059007C3","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1007\/s00418-016-1504-x","article-title":"Chemosensory epithelial cells in the urethra: sentinels of the urinary tract","volume":"146","author":"Deckmann","year":"2016","journal-title":"Histochem. 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