{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:02:47Z","timestamp":1753884167588,"version":"3.41.2"},"reference-count":29,"publisher":"The Company of Biologists","issue":"7","license":[{"start":{"date-parts":[[2022,7,6]],"date-time":"2022-07-06T00:00:00Z","timestamp":1657065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100001395","name":"Wisconsin Alumni Research Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001395","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007923","name":"University of Wisconsin Carbone Cancer Center","doi-asserted-by":"crossref","award":["P30 CA014520"],"award-info":[{"award-number":["P30 CA014520"]}],"id":[{"id":"10.13039\/100007923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007015","name":"University of Wisconsin Madison","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007015","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Epidemiological observations have prompted some to posit that elevated circulating vitamin D is responsible for reduced colon cancer in individuals residing near the equator. We have previously demonstrated that vitamin D has no effect on colon cancer in two rodent models of intestinal tumorigenesis. We have now extended this line of inquiry to ask whether ablation of vitamin D receptor (VDR) affects tumorigenesis. A VDR null rat was developed using Cas9-CRISPR technology, which allowed us to investigate whether 1,25(OH)D3 signaling through its receptor plays a role in intestinal tumorigenesis. Loss of VDR expression alone did not induce tumorigenesis, even in animals exposed to the inflammatory agent dextran sodium sulfate. These VDR\u2212\/\u2212 rats were then crossed with ApcPirc\/+ rats, which are predisposed to the development of intestinal neoplasms. In combination with the Pirc\/+ mutation, VDR loss did not enhance tumor multiplicity, growth, or progression in the colon or small intestine. This study demonstrates that the vitamin D receptor does not impact tumor development, and strongly supports previous findings that vitamin D itself does not play a role in colon cancer development or progression. Alternative explanations are needed for the original latitude hypothesis, as well as observational data in humans.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/bio.059290","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T08:46:25Z","timestamp":1654505185000},"source":"Crossref","is-referenced-by-count":6,"title":["Vitamin D receptor absence does not enhance intestinal tumorigenesis in <i>ApcPirc\/+<\/i>rats"],"prefix":"10.1242","volume":"11","author":[{"given":"Amy A.","family":"Irving","sequence":"first","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Biochemistry , , 433 Babcock Drive, Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bayley J.","family":"Waters","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Biochemistry , , 433 Babcock Drive, Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy R.","family":"Seeman","sequence":"additional","affiliation":[{"name":"Organic Lab, DiaSorin Inc 2 , 1951 Northwestern Avenue, Stillwater, MN 55082 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori A.","family":"Plum","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Biochemistry , , 433 Babcock Drive, Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3928-3303","authenticated-orcid":false,"given":"Hector F.","family":"DeLuca","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Biochemistry , , 433 Babcock Drive, Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2022,7,6]]},"reference":[{"key":"2022080314235055900_BIO059290C1","doi-asserted-by":"publisher","first-page":"4036","DOI":"10.1073\/pnas.0611690104","article-title":"A target-selected Apc-mutant rat kindred enhances the modeling of familial human colon cancer","volume":"104","author":"Amos-Landgraf","year":"2007","journal-title":"Proc. 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