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Despite of its importance for human health, little is known about the early stages of EV71 infection. EV71 starts uncoating with its receptor, human scavenger receptor B2 (hSCARB2), at low pH. We show that EV71 was not targeted to lysosomes in human rhabdomyosarcoma cells overexpressing hSCARB2 and that the autophagic pathway is not essential for EV71 productive uncoating. Instead, EV71 was efficiently uncoated 30\u2005min after infection in late endosomes (LEs) containing hSCARB2, mannose-6-phosphate receptor (M6PR), RAB9, bis(monoacylglycero)phosphate and lysosomal associated membrane protein 2 (LAMP2). Furthering the notion that mature LEs are crucial for EV71 uncoating, cation-dependent (CD)-M6PR knockdown impairs EV71 infection. Since hSCARB2 interacts with cation-independent (CI)-M6PR through M6P-binding sites and CD-M6PR also harbor a M6P-binding site, CD-M6PR is likely to play important roles in EV71 uncoating in LEs.<\/jats:p>","DOI":"10.1242\/bio.059469","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T10:15:19Z","timestamp":1659694519000},"source":"Crossref","is-referenced-by-count":15,"title":["The uncoating of EV71 in mature late endosomes requires CD-M6PR"],"prefix":"10.1242","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2394-6440","authenticated-orcid":false,"given":"Seii","family":"Ohka","sequence":"first","affiliation":[{"name":"Tokyo Metropolitan Institute of Medical Science 1 Neurovirology Project , , 156-8506, Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0368-3268","authenticated-orcid":false,"given":"Soon Hao","family":"Tan","sequence":"additional","affiliation":[{"name":"University of Malaya 2 Department of Pathology , , 59100, Kuala Lumpur , Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Ishiyama","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University 3 , 028-3694, Iwate , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsutoshi","family":"Ogasawara","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University 3 , 028-3694, Iwate , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohito","family":"Hanasaka","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University 3 , 028-3694, Iwate , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kinji","family":"Ishida","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University 3 , 028-3694, Iwate , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoji","family":"Hagiwara","sequence":"additional","affiliation":[{"name":"Tokyo Metropolitan Institute of Medical Science 1 Neurovirology Project , , 156-8506, Tokyo , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Chyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Vaccine R&D center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes 4 , Zhunan Town, Miaoli County , 35053, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pele Choi-Sing","family":"Chong","sequence":"additional","affiliation":[{"name":"Vaccine R&D center, National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes 4 , Zhunan Town, Miaoli County , 35053, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken-ichi","family":"Hanaki","sequence":"additional","affiliation":[{"name":"Technical Support Center for Life Science Research, Iwate Medical University 3 , 028-3694, Iwate , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4319-8745","authenticated-orcid":false,"given":"Giampietro","family":"Schiavo","sequence":"additional","affiliation":[{"name":"Molecular NeuroPathobiology Laboratory, Queen Square Institute of Neurology, University College London 5 , London , WC1N 3BG, United Kingdom"},{"name":"UK DRI Group, UK Dementia Research Institute at UCL 6 , London , WC1N 3AR, United Kingdom"},{"name":"UCL Queen Square Motor Neuron Disease Centre, University College London 7 , London WC1N 3BG , United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,9,13]]},"reference":[{"key":"2022091310231054300_BIO059469C1","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.jneumeth.2005.11.007","article-title":"Improved methods for ultracryotomy of CNS tissue for ultrastructural and immunogold analyses","volume":"153","author":"Akagi","year":"2006","journal-title":"J. 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