{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:50:36Z","timestamp":1760986236299,"version":"3.41.2"},"reference-count":46,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"crossref","award":["2016\/04085-9"],"award-info":[{"award-number":["2016\/04085-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de aperfei\u00e7oamento de Pessoal de N\u00edvel Superior - Brasil","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Universidade Estadual Paulista Julio de Mesquita Filho"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Maternal protein restriction delays the differentiation of epididymal mesenchymal cells in newborn rats. However, it is unclear whether this delay persists until the full differentiation of the epididymal epithelium at 44\u2005days postnatal. Thus, this study aimed to assess the impact of maternal protein reduction on 44-day-old rats\u2019 epididymal epithelium differentiation, following up on the observed delay in newborn animals. Pregnant rats were randomly divided into groups receiving normal-protein (NP: 17% protein) or low-protein (LP: 6% protein) diets during gestation and lactation. On postnatal day (PDN) 44, male offspring were euthanized, and the epididymis (NP n=10, LP n=10) was processed according to immunohistochemical techniques for the detection of aquaporin 9 (AQP9), KI-67, TP63, and ATPase. LP rats showed a decrease in the intensity of the AQP9 reaction, an increase in cellular proliferation in the initial segment and corpus of the epididymis, an increase in basal cells in the caput and corpus epididymis, and an increase in ATPase-positive clear cells in the cauda region. These findings demonstrate that maternal protein restriction impacts cell differentiation in the epididymal epithelium of 44-day-old rats, persisting even with a normal-protein diet after weaning.<\/jats:p>","DOI":"10.1242\/bio.060080","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T12:38:55Z","timestamp":1707309535000},"source":"Crossref","is-referenced-by-count":1,"title":["Maternal protein restriction affects the differentiation of cells in the epididymal epithelium lining of 44-day-old rats"],"prefix":"10.1242","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1272-5335","authenticated-orcid":false,"given":"F\u00e1bio","family":"Colonheze","sequence":"first","affiliation":[{"name":"Marilia Medical School 1 Graduate Program in Health and Aging , , Marilia, SP 17213-000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8713-0454","authenticated-orcid":false,"given":"Marilia Martins","family":"Cavariani","sequence":"additional","affiliation":[{"name":"Institute of Biosciences of Botucatu, S\u00e3o Paulo State University (UNESP) 2 Department of Structural and Functional Biology , , S\u00e3o Paulo 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0837-9612","authenticated-orcid":false,"given":"Bruno Cesar","family":"Schimming","sequence":"additional","affiliation":[{"name":"Institute of Biosciences of Botucatu, S\u00e3o Paulo State University (UNESP) 2 Department of Structural and Functional Biology , , S\u00e3o Paulo 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3885-1551","authenticated-orcid":false,"given":"Talita de Mello","family":"Santos","sequence":"additional","affiliation":[{"name":"Institute of Biosciences of Botucatu, S\u00e3o Paulo State University (UNESP) 2 Department of Structural and Functional Biology , , S\u00e3o Paulo 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0199-3396","authenticated-orcid":false,"given":"Luiz Gustavo de Almeida","family":"Chuffa","sequence":"additional","affiliation":[{"name":"Institute of Biosciences of Botucatu, S\u00e3o Paulo State University (UNESP) 2 Department of Structural and Functional Biology , , S\u00e3o Paulo 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-010X","authenticated-orcid":false,"given":"Raquel Fantin","family":"Domeniconi","sequence":"additional","affiliation":[{"name":"Institute of Biosciences of Botucatu, S\u00e3o Paulo State University (UNESP) 2 Department of Structural and Functional Biology , , S\u00e3o Paulo 18618-689 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"2025060605184447500_BIO060080C1","doi-asserted-by":"publisher","first-page":"1061","DOI":"10.1071\/RD13301","article-title":"Are the basal cells of the mammalian epididymis still an enigma?","volume":"26","author":"Arrighi","year":"2014","journal-title":"Reprod. 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