{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:39:20Z","timestamp":1787243960060,"version":"build-2736575974"},"reference-count":78,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"RIKEN BDR Research Center"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Historically, necrosis has been considered a passive process, which is induced by extreme stress or damage. However, recent findings of necroptosis, a programmed form of necrosis, shed a new light on necrosis. It has been challenging to detect necrosis reliably in vivo, partly due to the lack of genetically encoded sensors to detect necrosis. This is in stark contrast with the availability of many genetically encoded biosensors for apoptosis. Here we developed Necrosensor, a genetically encoded fluorescent sensor that detects necrosis in Drosophila, by utilizing HMGB1, which is released from the nucleus as a damage-associated molecular pattern (DAMP). We demonstrate that Necrosensor is able to detect necrosis induced by various stresses in multiple tissues in both live and fixed conditions. Necrosensor also detects physiological necrosis that occurs during spermatogenesis in the testis. Using Necrosensor, we discovered previously unidentified, physiological necrosis of hemocyte progenitors in the hematopoietic lymph gland of developing larvae. This work provides a new transgenic system that enables in vivo detection of necrosis in real time without any intervention.<\/jats:p>","DOI":"10.1242\/bio.060104","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T05:44:07Z","timestamp":1703828647000},"source":"Crossref","is-referenced-by-count":8,"title":["Necrosensor: a genetically encoded fluorescent sensor for visualizing necrosis in\n                    <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"13","author":[{"given":"Hiroshi","family":"Nishida","sequence":"first","affiliation":[{"name":"Graduate School of Medicine, Kobe University 1 Division of Cell Physiology , , Kobe, 650-0017, Japan"},{"name":"RIKEN CPR 2 Physiological Genetics Laboratory , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Bolea","family":"Albero","sequence":"additional","affiliation":[{"name":"RIKEN BDR 3 Laboratory for Epithelial Morphogenesis , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenta","family":"Onoue","sequence":"additional","affiliation":[{"name":"RIKEN BDR 4 Laboratory for Ultrastructural Research , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8956-0391","authenticated-orcid":false,"given":"Yuko","family":"Ikegawa","sequence":"additional","affiliation":[{"name":"Kyoto University 5 Laboratory of Molecular Cell Biology and Development , , Kobe, 650-0047, Japan"},{"name":"RIKEN BDR 6 Laboratory for Homeodynamics , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3605-6394","authenticated-orcid":false,"given":"Shivakshi","family":"Sulekh","sequence":"additional","affiliation":[{"name":"RIKEN BDR 6 Laboratory for Homeodynamics , , Kobe, 650-0047, Japan"},{"name":"Graduate School of Medicine, Kobe University 7 Division of Developmental Biology and Regenerative Medicine , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugurcan","family":"Sakizli","sequence":"additional","affiliation":[{"name":"RIKEN BDR 6 Laboratory for Homeodynamics , , Kobe, 650-0047, Japan"},{"name":"Graduate School of Medicine, Kobe University 7 Division of Developmental Biology and Regenerative Medicine , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Minami","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Kobe University 1 Division of Cell Physiology , , Kobe, 650-0017, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigenobu","family":"Yonemura","sequence":"additional","affiliation":[{"name":"RIKEN BDR 4 Laboratory for Ultrastructural Research , , Kobe, 650-0047, Japan"},{"name":"Tokushima University Graduate School of Medicine 8 Department of Cell Biology , , Tokushima, 770-8503, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chiun","family":"Wang","sequence":"additional","affiliation":[{"name":"RIKEN BDR 3 Laboratory for Epithelial Morphogenesis , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3358-4818","authenticated-orcid":false,"given":"Sa Kan","family":"Yoo","sequence":"additional","affiliation":[{"name":"RIKEN CPR 2 Physiological Genetics Laboratory , , Kobe, 650-0047, Japan"},{"name":"RIKEN BDR 6 Laboratory for Homeodynamics , , Kobe, 650-0047, Japan"},{"name":"Graduate School of Medicine, Kobe University 7 Division of Developmental Biology and Regenerative Medicine , , Kobe, 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"2024012214584306900_BIO060104C1","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1038\/nprot.2006.235","article-title":"Detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and acridine orange in Drosophila embryos and adult male gonads","volume":"1","author":"Arama","year":"2006","journal-title":"Nat. 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